List of wild Hieracium subgen. Pilosella plants used for genome size estimation. Detailed information is given about original locality (including

Supplementary Information List of wild Hieracium subgen. Pilosella plants used for genome size estimation. Detailed information is given about original locality (including geographic co-ordinates and altitude), collector(s), year of collection, and name(s) of hawkweed specialists revising plant samples together with somatic chromosome numbers and breeding systems for 278 accessions (184 basic species, 87 intermediate = hybridogenous species, and seven recent natural crosses) Accession Locality*, collector(s), year of collection, taxonomic revision Latitude Longitude Altitude Chromosome Breeding number N E number (2n) system Basic species H. aurantiacum L., 2n = 4x 119 AU CZ, Krkonoše Mts, Horní Mísečky, FK & AK 1997, rev. SB 50 44'10'' 15 34'10'' 1060 m 36 a apomictic 134/1 AU CZ, Krkonoše Mts, Velká Úpa - village, FK 1998, rev. SB 50 41'30'' 15 45'00'' 760 m 36 b NS 136 AU CZ, Krkonoše Mts, Hlušiny, FK 1998, rev. SB 50 40'50'' 15 46'30'' 930 m 36 a NS 192 AU CZ, Krkonoše Mts, Pec pod Sněžkou, FK 1999, rev. SB 50 41'50'' 15 43'50'' 830 m 36 b NS 20 AU CZ, Krkonoše Mts, Velká Úpa, Prostřední Výsluní, FK & AK 1997, rev. SB 50 41'40'' 15 47'10'' 910 m 36 a apomictic a 223 AU CZ, Krkonoše Mts, Dolní Malá Úpa, Moravanka, FK, AK, SB & JC1999 50 43'50'' 15 49'10'' 1050 m 36 b NS 252 AU CZ, Šumava Mts, former settlement of Gerlova Huť near Hartmanice, FP 1999, rev. SB & FS 49 09'54'' 13 16'44'' 950 m 36 c NS 270 AU CZ, Šumava Mts, Kvilda, FK 1999 49 00'30'' 13 34'10'' 1080 m 36 c NS 30 AU CZ, Krkonoše Mts, Velká Úpa - Janovy Boudy, FK 1995, rev. SB 50 41'20'' 15 48'00'' 860 m 36 a apomictic a 387 AU CZ, Krušné hory Mts, Přebuz, FK 2000 50 24'00'' 12 30'00'' 890 m 36 d NS 94 AU CZ, Krkonoše Mts, Horní Malá Úpa Pomezní Boudy, FK 1997, rev. SB 50 44'10'' 15 48'10'' 950 m 36 a NS 451 AU CZ, Krkonoše Mts, in the valley below Dolní Malá Úpa FK, AK & JC 2000, rev. SB 50 42'50'' 15 48'50'' 880 m 36 NS 546a AU CZ, Šumava Mts, Hadí vrch near Hartmanice, FP & FK 2001, rev. SB & FS 49 11'05'' 13 20'45'' 960 m 36 NS 895 AU CZ, Šumava Mts, Slučí Tah near Hartmanice, FK 2003, rev. SB & FS 49 09'10'' 13 21'00'' 880 m NC apomictic 905 AU CZ, Šumava Mts, Zhůří near Železná Ruda, FK 2003, rev. SB & FS 49 10'45'' 13 20'10'' 930 m NC NS 576a AU CZ, Ještědský hřeben Mts, Kryštofovo Údolí near Chrastava, FK & SB 2001 50 46'04'' 14 55'57'' 440 m NC NS 842 AU D, North Rhine Westfalia, Hagen - Eilpe, Krähnocken, above Eilper Hangstieg, UR 2003 51 21' 7 28' 260 m NC NS 838 AU D, North Rhine Westfalia, Hagen - Eilpe, Krähnocken, above Eilper Hangstieg, UR 2003 51 21' 7 28' 260 m NC NS 1

H. aurantiacum L., 2n = 5x 135 AU CZ, Krkonoše Mts, Velká Úpa, FK 1998, rev. SB 50 41'20'' 15 45'30'' 720 m 45 b NS 265 AU CZ, Krkonoše Mts, Zadní Rennerovky, FK 1999, rev. SB 50 42'00'' 15 40'00'' 1240 m 45 b apomictic b 915 AU CZ, Krkonoše Mts, Rýchory, FK 2003, rev. SB 50 39'10'' 15 52'10'' 960 m 45 NS 769/1 AU CZ, Šumava Mts, Zhůří near Kašperské Hory, FP, FK, SB & FS 2002 49 05'10'' 13 33'20'' 1120 m NC apomictic 804 AU A, Allgäuer Alpen Mts, Wildental, HWS 2002 47 17'40'' 10 11'10'' 1900 m NC NS 824 AU/3 RO, Tarcului Mtii Mts, Tomeasa Mt, PM 2002 45 21'15'' 22 42'10'' 1800 m NC NS H. bauhinii Besser, 2n = 4x 491 BA CZ, Praha Vysočany, near the railway station, FK 2001 50 06'45'' 14 29'50'' 210 m 36 apomictic 591/1 BA CZ, Olomouc - Holice, FK 2002 49 34'40'' 17 17'00'' 210 m NC NS H. bauhinii Besser, 2n = 5x 589 BA SK, Velká Fatra Mts, Tlstá Mt, FK 2001 48 56'00'' 18 58'30'' 1370 m NC NS 607 BA CZ, Lovosice, Malé Žernoseky, FK 2002 50 32'25'' 14 03'15'' 200 m NC apomictic 615 BA CZ, Roudnice, Drahobuz, FK 2002 50 31'30'' 14 20'20'' 220 m NC NS 655 BA CZ, Praha Vysočany, near the railway station, FK & AK 2002 50 06'45'' 14 29'50'' 210 m 45 apomictic 659 BA CZ, Praha Vysočany, near the railway station, FK & AK 2002 (a clone different from 655) 50 06'45'' 14 29'50'' 210 m 45 NS 676 BA CZ, Dolní Dunajovice near Mikulov, FK, OR & JD 2002 48 51'20'' 16 33'05'' 240 m NC apomictic 687 BA CZ, Sedlec near Mikulov, FK, OR & JD 2002 48 46'45'' 16 40'20'' 190 m NC NS 718 BA CZ, Lomnice nad Popelkou, Ploužnice, FK 2002 50 30'40'' 15 23'10'' 520 m NC NS 720 BA CZ, Rovensko pod Troskami - Semínova Lhota, FK 2002 50 31'05'' 15 16'30'' 340 m NC apomictic H. bauhinii Besser, 2n = 6x 126 BA CZ, Valov near Podbořany, FK 1998 50 12'50'' 13 25'00'' 350 m 54 c apomictic c 463 BA CZ, Rozkoš near Česká Skalice, FK 2000 50 23'00'' 16 02'45'' 300 m 54 NS 700 BA CZ, Brno, Kamenný kopec hill, FK & OR 2002 49 10'50'' 16 33'15'' 370 m NC apomictic H. bauhinii Besser, 2n = 7x 492 BA CZ, Praha Vysočany, near the railway station, FK 2001 50 06'45'' 14 29'50'' 210 m 63 apomictic H. caespitosum Dumort., 2n = 4x 113 CE CZ, Krkonoše Mts, Pec pod Sněžkou, FK & AK 1997, rev. SB 50 41'20'' 15 43'10'' 910 m 36 a apomictic b 358 CE PL, Góry Izerskie Mts, Velká Jizerská louka meadow close to Czech-Polish border, FK 2000, 50 51'00'' 15 21'40'' 830 m 36 d NS rev. HWS 364 CE D, East Lusatian Hills, Piessnitz valley near Schönau-Berzdorf, FK & AK 2000, rev. HWS 51 03'35'' 14 54'04'' 220 m 36 d NS 369 CE D, East Lusatian Hills, eastern edge of Schönau-Berzdorf, FK & AK 2000, rev. HWS 51 03'50'' 14 53'50'' 200 m 36 d NS 452 CE CZ, Krkonoše Mts, Velká Úpa, FK, AK & JC 2000, rev. SB 50 41'53'' 15 46'05'' 900 m 36 NS 748 CE CZ, Šumava Mts, Hadí vrch near Hartmanice, FP & FK 2001, rev. SB & FS 49 11'05'' 13 20'45'' 960 m NC NS 757 CE CZ, Šumava Mts, Slučí Tah near Hartmanice, FP & FK 2001, rev. SB & FS 49 09'10'' 13 21.00' 880 m NC NS 881 CE CZ, Šumava Mts, Zhůří near Kašperské Hory, FK 2003, rev. SB & FS 49 05'10'' 13 33'20'' 1120 m NC NS 2

H. caespitosum Dumort., 2n = 5x caec. Hir 3 D, Erzgebirge Mts, Hirtstein, SB 2000 50 32'02'' 13 10'24'' 880 m NC NS H. cymosum L. subsp. cymosum, 2n = 2x 10/3 CY CZ, Křivoklátsko, Nižbor, at the rock Čerchov, RŠ 1999 50 00'18'' 13 58'44'' 240 m 18 (counted by RŠ) sexual 11/12 CY CZ, Křivoklátsko, between Račice and Zbečno, RŠ 1999 50 01'34'' 13 54'30'' 250 m 18 (counted by RŠ) sexual 13/7 CY CZ, České středohoří Mts, Bělušice, Milá hill, RŠ 1999 50 26'02'' 13 45'24'' 510 m 18 (counted by RŠ) sexual 14/11 CY CZ, České středohoří Mts, Lovosice, Boreč hill, RŠ 1999 50 30'55'' 13 59'12'' 420 m 18 (counted by RŠ) sexual 15/9 CY CZ, Bohemian Karst, between Karlštejn and Zadní Třebáň, RŠ 1999 49 55'01'' 14 11'45'' 300 m 18 (counted by RŠ) sexual 16/4 CY CZ, Praha-Bohnice, RŠ 1999 50 08'30'' 14 23'58'' 190 m 18 (counted by RŠ) sexual 2/5 CY CZ, Zrůbek, at the Slapská přehrada reservoir, RŠ 1998 49 40'43'' 14 17'30'' 270 m 18 (counted by RŠ) sexual 20/3 CY CZ, Moravský Krumlov, Sv. Florián hill, RŠ 1999 49 02'51'' 16 19'10'' 280 m 18 (counted by RŠ) sexual 3/2 CY CZ, Cholín, at the Slapská přehrada reservoir, RŠ 1998 49 43'22'' 14 19'36'' 350 m 18 (counted by RŠ) sexual 4/3 CY CZ, Labské středohoří Mts, Sebuzín, Havraní skála, RŠ 1998 50 35'34'' 14 04'26'' 390 m 18 (counted by RŠ) sexual 42/3 CY SK, Slanské vrchy hills, Slanec, Slanský hradný vrch hill, RŠ 2000 48 38'11'' 21 28'22'' 520 m 18 (counted by RŠ) sexual 43/1 CY SK, Slovenské rudohorie Mts, Jelšavská Teplica, Muteň hill, RŠ 2000 48 36'00'' 20 18'00'' 400 m 18 (counted by RŠ) sexual 44/2 CY SK, Štiavnické vrchy, Hliník nad Hronom, nature reserve Kapitulské bralá, RŠ 2000 48 32'33'' 18 51'00'' 320 m 18 (counted by RŠ) sexual 48/5 CY CZ, Tasovice, Tasovické svahy above Dyje river, RŠ & JC 2000 48 49'51'' 16 08'21'' 200 m 18 (counted by RŠ) sexual 6/9 CY CZ, Výří skály near Tukleky, near the Orlík reservoir, RŠ 1999 49 23'26'' 14 11'10'' 420 m 18 (counted by RŠ) sexual 7/10 CY CZ, Oslov near the Orlík reservoir, Krkavčina pine-wood, RŠ 1999 49 24'00'' 14 11'06'' 420 m 18 (counted by RŠ) sexual 9/5 CY CZ, Bohemian Karst, Srbsko, Velká hora hill, RŠ 1999 49 56'56'' 14 09'25'' 350 m 18 (counted by RŠ) sexual H. cymosum L. subsp. cymosum, 2n = 4x 21/6 CY CZ, Adamov 1, slopes above the Svitava river, RŠ 1999 49 17'45'' 16 40'07'' 300 m NC NS 22/7 CY CZ, Adamov 2, slopes above the Svitava river, RŠ 1999 49 17'47'' 16 40'08'' 320 m NC NS 47/5 CY CZ, Lukov, Vraní skála rock above the Dyje river, RŠ & JC 2000 48 50'59'' 15 53'45'' 360 m 36 NS H. cymosum L. subsp. cymigerum Reichenb., 2n = 5x 722 CY CZ, Krkonoše Mts, Víchovská Lhota, FK 2002, rev. SB 50 38'55'' 15 29'15'' 580 m 45 NS H. echioides L., 2n = 2x 30/2 A, Niederösterreich, 1 km N of the village of Markhof, TP, JC & DH 1999 48 16 43 16 50 19 150 m 18 c sexual 31/1 A, Niederösterreich, Mt. Braunsberg near Hainburg an der Donau, TP, JC & DH 1999 48 09 31 16 57 14 320 m 18 c sexual 37/c CZ, P: the valley of the Dyje river SE of the village of Dyje, TP 1999 48 48 40 15 59 45 240 m 18 c sexual 39/a CZ, Cínová hora hill near Znojmo, TP 1999 48 52 08 16 00 33 315 m 18 c sexual 48/e CZ, hill at the NE margin of Hostěradice, TP 2000 48 57 12 16 15 46 240 m 18 c sexual 51/a D, Brandenburg, distr.: Barnim: Pimpinellenberg hill near Oderberg, TP & JC 2000 52 52 13 14 01 32 50 m 18 c sexual 56/3 PL, Skorocice, the nature reserve near the village, TP, JC & ZS 2001 50 29 20 50 210 m NC sexual 58/1 PL, Przemyśl, along the road from the town to the Ukrainian border, TP, JC & ZS 2001 49 47 22 50 190 m NC sexual 58/23 PL, Przemyśl, along the road from the town to the Ukrainian border, TP, JC & ZS 2001 49 47 22 50 190 m NC sexual 3

59/2 SK, Tarbucka hill in Zemplínske vrchy Mts, TP & JC 2001 48 22 27 21 46 59 180 m NC sexual 83/1 H, Fenyőfő in Bakony Mts, TP & JC 2002 47 17 17 45 350 m 18 (counted by TP) sexual 84/3 H, Fenyőfő in Bakony Mts, TP & JC 2002 47 17 17 45 350 m NC sexual 85/2 H, Fenyőfő in Bakony Mts, TP & JC 2002 47 17 17 45 350 m NC sexual 93/1 CZ, České středohoří Mts, Litoměřice, Radobýl hill, TP & HWS 2003 50 13 44 14 05 34 350 m NC sexual HAV S 54 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC 2001 48 48 40 15 59 45 300 m 18 (counted by TP) sexual HV 4 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC 2003 48 48 40 15 59 45 300 m NC sexual H. echioides L., 2n = 3x 22/d CZ, between Konice and Popice near Znojmo, TP 1998 48 49 47 N 16 01 08 290 m 27 c sexual HAV J 22 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC 2001 48 48 40 15 59 45 300 m 27 (counted by TP) sexual HAV J 33 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC 2001 48 48 40 15 59 45 300 m 27 (counted by TP) sexual HAV S 59 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC 2001 48 48 40 15 59 45 300 m NC sexual HV 10 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC 2003 48 48 40 15 59 45 300 m 27 (counted by TP) sexual HV 24 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC 2003 48 48 40 15 59 45 300 m NC sexual HV 27 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC 2003 48 48 40 15 59 45 300 m 27 (counted by TP) sexual HV 41 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC 2003 48 48 40 15 59 45 300 m 27 (counted by TP) sexual HV 5 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC 2003 48 48 40 15 59 45 300 m NC sexual HV 6 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC 2003 48 48 40 15 59 45 300 m 27 (counted by TP) sexual H. echioides L., 2n = 4x 30/3 A, Niederösterreich, 1 km N of the village of Markhof, TP, JC & DH 1999 48 16 43 16 50 19 150 m 36 c sexual 4/3 CZ, Šobes vineyards near Hnanice, TP1998 48 49 08 15 58 26 270 m 36 c sexual HAV J 1 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC 2001 48 48 40 15 59 45 300 m 36 (counted by TP) sexual HV 11 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC 2003 48 48 40 15 59 45 300 m NC sexual HV 12 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC 2003 48 48 40 15 59 45 300 m NC sexual HV 13 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC 2003 48 48 40 15 59 45 300 m NC sexual HV 14 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC 2003 48 48 40 15 59 45 300 m NC sexual HV 15 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC 2003 48 48 40 15 59 45 300 m NC sexual HV 20 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC 2003 48 48 40 15 59 45 300 m NC sexual HV 21 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC 2003 48 48 40 15 59 45 300 m 36 (counted by TP) sexual HV 29 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC 2003 48 48 40 15 59 45 300 m 36 (counted by TP) sexual HV 34 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC 2003 48 48 40 15 59 45 300 m NC sexual H. echioides L., 2n = 5x 38/a CZ, Havranické vřesoviště heathland near Havraníky, TP & JC 2003 48 48 40 15 59 45 300 m 45 d sexual 7/2 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC 2003 48 48 40 15 59 45 300 m 45 (counted by TP) sexual HV 28 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC 2003 48 48 40 15 59 45 300 m 45 d sexual HV 30 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC 2003 48 48 40 15 59 45 300 m NC sexual 4

H. hoppeanum Schultes, 2n = 2x 781 HO D, Ausburg, Nature reserve Ausburger Wald, near Mering, MC 2001 48 16'25'' 10 54'15'' 510 m NC NS H. hoppeanum subsp. testimoniale Nägeli ex Peter (syn. H. macranthum (Ten.) Ten.), 2n = 2x 948 MC BG, Vračanska Planina Mts, between Vratsa and Parševitsa hut, FK, AK & VV 2005, rev. SB 43 10'35'' 23 28'55'' 1050 m NC NS 962 MC BG, Vračanska Planina Mts, between Vratsa and Parševitsa hut, FK, AK & VV 2005, rev. SB 43 10'15'' 23 28'50'' 1160 m NC NS 969 MC BG, near the dam of Černila, S of the town of Montana, FK, AK & VV 2005, rev. SB 43 22'30'' 23 14'40'' 190 m NC NS 1008 MC BG, Eastern Rodopi Mts, E of the town of Kardžali, FK, AK & VV 2005, rev. SB 41 38'10'' 25 25'50'' 260 m 18 (counted by VV) sexual H. lactucella Wallr., 2n = 2x 259 LA SK, Velká Fatra Mts, Malá Krížna Mt, FK & AK 1999, rev. HWS 48 52'40'' 19 03'40'' 1500 m NC NS 260 LA SK, Západné Tatry Mts, Osobitá Mt, FK & AK 1999, rev. HWS 49 15'40'' 19 43'20'' 1570 m 18 c NS 269 LA CZ, Šumava Mts, Kvilda, FK 1999, rev. SB & FS 49 00'40'' 13 34'40'' 1050 m 18 c NS 272 LA CZ, Šumava Mts, Gerlova Huť near Železná Ruda, FK 1999, rev. SB & FS 49 10'00'' 13 20'00'' 920 m 18 c NS 277 LA CZ, Krkonoše Mts, Velká Úpa - Janovy Boudy, FK 1999, rev. SB 50 41'20'' 15 48'00'' 860 m 18 NS 44 LA CZ, Krkonoše Mts, Velká Úpa - Janovy Boudy, FK 1995, rev. SB 50 41'20'' 15 48'00'' 860 m 18 a sexual a 436 LA CZ, Krkonoše Mts, Horní Malá Úpa Pomezní Boudy, FK, AK & JC 2000, rev. SB 50 44'20'' 15 48'50'' 1000 m 18 NS 529 LA CZ, Brdy Hills, Dolní Kvaň near Hořovice, JH 2001 49 45'10'' 13 51'00'' 520 m 18 d NS 786 LA CZ, Českomoravská vrchovina Highlands, Cikháj, OR 2001 49 39'07'' 15 57'54'' 650 m 18 c NS 787 LA SK, Velká Fatra Mts, Baník hill near Donovaly, OR 2001 48 52'50'' 19 13'30'' 1060 m 18 d NS 789 LA D, Oberpfalzer Wald, Wümberg-Köblitz, FK 2002 49 33'00'' 12 11'40'' 460 m NC NS 790 LA A, Allgäuer Alpen Mts, Massife of Widderstein Mt, FK & AK 2002, rev. HWS 47 16'15'' 10 07'00'' 1900 m NC NS 806 LA A, Allgäuer Alpen Mts, W of Walmendiger Horn Mt near Baad, FK & AK 2002, rev. HWS 47 19'55'' 10 07'15'' 1450 m NC NS 865 LA CZ, Šumava Mts, Jelení Vrchy, FK 2003, rev. SB & FS 48 57'00'' 13 47'10'' 820 m NC NS 889 LA CZ, Šumava Mts, Filipova Huť, FK 2003, rev. SB & FS 49 01'25'' 13 30'40'' 1060 m NC NS lac.hot 1 D, Erzgebirge Mts, Schellerhau - Hotel, SB 2000 50 46'38'' 13 41'24'' 770 m NC NS lac. Jon 2 D, Oberlausitz, Jonsdorf, SB 1999 50 50'34'' 14 42'30'' 510 m NC NS lac. Neu 1 D, Erzgebirge Mts, Neuwernsdorf, SB 2000 50 42'07'' 13 31'17'' 600 m NC NS lac. ZwW 1 D, Erzgebirge Mts, Zweibach West, SB 2000 50 27'04'' 12 52'20'' 770 m NC NS H. onegense (Norrl.) Norrl., 2n = 2x 261 CE/2 SK, Západné Tatry Mts, near Zverovka chalet, FK & AK 1999, rev. HWS 49 17'00'' 19 42'00'' 1080 m 18 c NS 6/4 SP2 CZ, Krkonoše Mts, Velká Úpa - Janovy Boudy, FK & AK 1998, rev. SB 50 41'20'' 15 47'50'' 870 m 18 b sexual b RS 17/6 CZ, Hodonín, the nature reserve Dúbrava, RS 2000 48 52'18'' 17 06'06'' 170 m 18 (counted by RŠ) NS 946 ON BG, Vračanska Planina Mts, between Vratsa and Parševitsa hut, FK, AK & VV 2005, rev. SB 43 10'35'' 23 28'55'' 1050 m NC NS 975 ON BG, Petrochan saddle S of Berkovitsa, FK, AK & VV 2005, rev. SB 43 07'05'' 23 07'35'' 1390 m NC NS H. pavichii Heuffel, 2n = 2x 5

1004 PAV BG, Central Rodopi Mts, Komuniga village, near the settlement of Jončovo, FK, AK & VV 41 50'20'' 25 11'40'' 770 m NC sexual 2005, rev. SB H. pilosella L., 2n = 4x 69 PI CZ, Krkonoše Mts, Velká Úpa - Janovy Boudy, FK 1995, rev. SB 50 41'20'' 15 48'00'' 860 m 36 a sexual a 120 PI CZ, Krkonoše Mts, Horní Mísečky, FK & AK 1997, rev. SB 50 44'20'' 15 33'50'' 1070 m 36 a sexual a 158 PI CZ, Vršovka near Náchod, FK 1999 50 19'30'' 16 07'00'' 330 m 36 c NS 169 PI CZ, Malý Kosíř hill near Slatinice na Hané, FK 1999 49 33'30'' 17 05'40'' 270 m 36 c sexual c 191 PI CZ, Krkonoše Mts, Pec pod Sněžkou, FK 1999, rev. SB 50 41'50'' 15 43'50'' 830 m 36 b NS 218 PI CZ, Krkonoše Mts, Horní Malá Úpa Pomezní Boudy, FK, SB & JC 1999 50 44'10'' 15 48'00'' 950 m 36 b sexual b 241 PI CZ, Krkonoše Mts, Moravská bouda near Špindlerův Mlýn, FK, SB & JC 1999 50 46'10'' 15 36'30'' 1240 m 36 b sexual b 246 PI CZ, Krkonoše Mts, Benecko, FK, SB & JC 1999 50 40'00'' 15 33'00'' 800 m 36 b sexual b 268 PI CZ, Šumava Mts, Stožec, FK 1999, rev. SB & FS 48 51'30'' 13 49'20'' 780 m 36 c sexual 300 PI CZ, Praha Vysočany, near the railway station, FK & JC 2000 50 06'45'' 14 29'50'' 210 m 36 c sexual 362 PI PL, Góry Izerskie Mts, Jakuszyce, FK 2000, rev. HWS 50 48'45'' 15 23'50'' 830 m 36 d NS 376 PI CZ, Krušné hory Mts, Přebuz, FK 2000 50 24'00'' 12 30'00'' 890 m 36 d NS 427 PI CZ, Krkonoše Mts, Víchovská Lhota, FK, AK & JC 2000, rev. SB 50 38'55'' 15 29'15'' 580 m 36 NS 543 PI CZ, Šumava Mts, Hadí vrch near Hartmanice, FP & FK 2001, rev. SB & FS 49 11'05'' 13 20'45'' 960 m 36 sexual 551 PI CZ, Šumava Mts, Slučí Tah near Hartmanice, FP & FK 2001 49 09'10'' 13 21'00'' 880 m 36 NS 578 PI CZ, Ještědský hřeben Mts, Kryštofovo Údolí near Chrastava, FK & SB 2001 50 46'04'' 14 55'57'' 440 m 36 d NS 585 PI CZ, Bědovice near Týniště nad Orlicí, FK & AK 2001 50 11'35'' 16 01'50'' 250 m NC NS 640 PI CZ, České středohoří Mts, Raná hill, FK 2002 50 24'35'' 13 46'40'' 320 m NC NS 714 PI CZ, Vrbčany near Kouřim, FK 2002 50 03'40'' 14 60'00'' 220 m NC NS 715 PI CZ, Zebín hill near Jičín, FK 2002 50 27'15'' 15 22'20'' 390 m NC NS 5/97 PI PL, Beskid Sląski Mts, Ustroń, AK 1997 49 43'20'' 18 51'20'' 800 m 36 c NS 734 PI CZ, Šumava Mts, Jelení Vrchy, FP, FK, SB & FS 2002 48 57'00'' 13 47'10'' 820 m NC sexual 765 PI CZ, Šumava Mts, Strašín (limestone quarry), FP, FK, SB & FS 2002 49 11'00'' 13 37'50'' 590 m NC NS 782 PI CZ, Mnichov near Mariánské Lázně (serpentine rocks), OR 2000 50 03'12'' 12 47'07'' 700 m 36 c NS 836 PI D, North Rhine Westfalia, Hagen Eilpe, Krähnocken, above Eilper Hangstieg, UR 2003 51 21' 7 28' 260 m NC NS 841 PI D, North Rhine Westfalia, Hagen Eilpe, Krähnocken, above Eilper Hangstieg, UR 2003 51 21' 7 28' 260 m NC NS H. pilosella L., 2n = 5x 168 PI CZ, Malý Kosíř hill near Slatinice na Hané, FK 1999 49 33'30'' 17 05'40'' 270 m 45 c sexual c 273 PI CZ, Valašské Klobouky, FK 1999 49 08'10'' 18 02'10'' 510 m 45 c NS 294 PI CZ, Praha Vysočany, near the railway station, FK & JC 2000 50 06'45'' 14 29'50'' 210 m 45 c sexual 341 PI CZ, Olomouc - Holice, FK 2000 49 34'40'' 17 17'00'' 210 m 45 NS 581/1 PI SK, Nízke Tatry Mts, Borová Sihoť, FK 2001, rev. HWS 49 02'15'' 19 42'20'' 650 m NC NS 587 PI SK, Velká Fatra Mts, Necpalská dolina valley, FK 2001 48 58'30'' 19 00'00'' 560 m NC NS 6

588 PI SK, Velká Fatra Mts, Tlstá Mt, FK 2001 48 56'00'' 18 58'30'' 1370 m NC NS H. pilosella L., 2n = 6x 163 PI CZ, Běstviny near Rychnov nad Kněžnou, FK 1999 50 19'30'' 16 08'40'' 310 m 54 c apomictic c 263 PI SK, Velká Fatra Mts, Králova Studňa Mt, FK & AK 1999, rev. HWS 48 52'40'' 19 02'30'' 1270 m 54 c NS 582 PI SK, Chočské pohorie Mts, Prosiek, FK & AK 2001, rev. HWS 49 09'30'' 19 29'55'' 640 m 54 NS 669 PI CZ, Bílý kopec hill near Čejč, FK & VM 2002 48 56'25'' 16 59'05'' 190 m NC apomictic 675 PI CZ, Dolní Dunajovice near Mikulov, FK, OR & JD 2002 48 51'20'' 16 33'05'' 240 m NC apomictic 686 PI CZ, Sedlec near Mikulov, FK, OR & JD 2002 48 46'45'' 16 40'20'' 190 m NC apomictic 693 PI CZ, Brno, Kamenný kopec hill, FK & OR 2002 49 10'50'' 16 33'20'' 370 m 54 NS 712 PI CZ, Slavkov near Brno, Rašovice, FK & OR 2002 49 07'10'' 16 56'25'' 420 m 54 NS CM 3 PI CZ, Praha, Černý Most, VK 50 06'50'' 14 35'15'' 250 m NC apomictic H. pilosella L., 2n = 7x CM 7 PI CZ, Praha, Černý Most, VK 50 06'50'' 14 35'15'' 250 m 63 (counted by V. Jarolímová) NS Intermediate species H. bifurcum M. Bieb., 2n = 5x 72 (Šobes) CZ, Šobes vineyards near Hnanice, TP1998 48 49 08 15 58 26 270 m 45 d almost sterile H. blyttianum Fries, 2n = 4x 222 AUxLA CZ, Krkonoše Mts, Dolní Malá Úpa, Moravanka, FK, AK, SB & JCH 1999 50 43'50'' 15 49'10'' 1050 m 36 b apomictic b 198 AUxLA CZ, Krkonoše Mts, Horní Malá Úpa - Pomezní Boudy, FK, SB & JCH 1999 50 44'20'' 15 48'20'' 1010 m 36 b apomictic b H. brachiatum Bertol. ex DC., 2n = 4x 495 BRA CZ, Praha Vysočany, near the railway station, FK 2001 50 06'45'' 14 29'50'' 210 m 36 apomictic 642 BRA CZ, Praha Vysočany, near the railway station, FK & AK 2002 50 06'45'' 14 29'50'' 210 m 36 sexual 613 BRA CZ, Roudnice, Drahobuz, FK 2002 50 31'30'' 14 20'20'' 220 m NC sexual H. brachiatum Bertol. ex DC., 2n = 5x 667/1 BRA CZ, Kobeřice near Slavkov, FK & OR 2002 49 05'35'' 16 53'20'' 330 m 45 apomictic 164 BRA CZ, Slatinice na Hané, FK 1999 49 33'40'' 17 05'40'' 250 m 45 c apomictic c H. brachiatum Bertol. ex DC., 2n = 7x 507 BRA CZ, Praha - Vysočany, near the railway station, FK 2001 (4 distinct clones 507,508,513,651) 50 06'45'' 14 29'50'' 210 m 63 apomictic 508 BRA CZ, Praha - Vysočany, near the railway station, FK 2001 50 06'45'' 14 29'50'' 210 m 63 apomictic 513 BRA CZ, Praha - Vysočany, near the railway station, FK 2001 50 06'45'' 14 29'50'' 210 m 63 apomictic 651 BRA CZ, Praha - Vysočany, near the railway station, FK & AK 2002 50 06'45'' 14 29'50'' 210 m 63 apomictic H. brachiatum Bertol. ex DC., 2n = 8x 695 BRA CZ, Brno, Kamenný kopec hill, OR 1999, FK & OR 2002 49 10'50'' 16 33'15'' 370 m 72 c apomictic 7

1025 BRA CZ, Praha - Vysočany, near the railway station, FK & JC 2000, FK &AK 2005 50 06 45 14 29 50 210 m 72 c apomictic c H. floribundum Wimm. et Grab., 2n = 4x 24 FL CZ, Krkonoše Mts, Velká Úpa - Janovy Boudy, FK 1995, rev. SB 50 41'20'' 15 48'00'' 870 m 36 a apomictic a 250 FL CZ, Šumava Mts, in the former settlement of Stará Hůrka, FP 1999, rev. SB & FS 49 07'30'' 13 19'48'' 1010 m 36 c NS 373 FL CZ, Krušné hory Mts, Přebuz, FK 2000 50 24'00'' 12 30'00'' 890 m 36 c NS 379 FL CZ, Krušné hory Mts, Přebuz, FK 2000 (the clone different from 373) 50 24'00'' 12 30'00'' 890 m 36 c NS 384 FL CZ, Krušné hory Mts, Rolava, FK 2000 50 23'15'' 12 35'45'' 880 m 36 c NS 466 FL CZ, Českomoravská vrchovina Highlands, Žďár nad Sázavou - railway station, OR 1998 49 33'14'' 15 56'20'' 560 m 36 c NS 468 FL PL, Kudowa Zdrój, Lewin Klodzki, RŠ 2000 50 24'30'' 16 16'50'' 450 m 36 c NS 879 FL CZ, Šumava Mts, Zhůří near Kašperské Hory, FK 2003, rev. SB & FS 49 05'10'' 13 33'20'' 1120 m NC NS 899 FL CZ, Šumava Mts, Zhůří near Železná Ruda, FK 2003, rev. SB & FS 49 10'50'' 13 20'20'' 950 m NC apomictic 912 FL CZ, Šumava Mts, Gerlova Huť near Železná Ruda, FK 2003, rev. SB & FS 49 09'54'' 13 16'44'' 950 m NC NS H. floribundum Wimm. et Grab., 2n = 5x 580/1 FL SK, Nízke Tatry Mts, Demänovská dolina valley, Jasná pod Chopkom, FK & AK 2001, rev. 48 57'55'' 19 34'55'' 1200 m NC NS HWS 783 FL PL, distr. Kraków, Gacki settlement near Wielka Wies, OR 2000 50 10'13'' 19 50'43'' 400 m NC NS H. glomeratum Froel., 2n = 4x 90 GL CZ, Krkonoše Mts, Horní Malá Úpa - Pomezní Boudy, FK 1997, rev. SB 50 44'20'' 15 48'10'' 960 m 36 a apomictic a H. glomeratum Froel., 2n = 5x 157 GL CZ, Vršovka near Náchod, FK 1999 50 19'30'' 16 07'00'' 330 m 45 c NS 576 GL CZ, Ještědský hřeben Mts, Kryštofovo Údolí near Chrastava, FK & SB 2001 50 46'04'' 14 55'57'' 440 m NC NS 767 GL CZ, Šumava Mts, Nové Hutě, FP, FK, SB & FS 2002 49 02'10'' 13 38'30'' 1010 m NC NS 875 GL CZ, Šumava Mts, Zhůří near Kašperské Hory, FK 2003, rev. SB & FS 49 04'50'' 13 33'20'' 1140 m NC NS 886 GL CZ, Šumava Mts, Filipova Huť, FK 2003, rev. SB & FS 49 01'25'' 13 30'40'' 1060 m NC NS 919 GL SK, Slovenský raj Mts, Stratenská Píla, HWS 2004 48 51'30'' 20 21'30'' 800 m NC NS H. iseranum Uechtr., 2n = 4x 117 IS CZ, Krkonoše Mts, Výrovka near Pec pod Sněžkou, FK & AK 1997, rev. SB 50 43'10'' 15 41'00'' 1390 m 36 a apomictic a 4/8 AP CZ, Krkonoše Mts, Velká Úpa - Janovy Boudy, FK & AK 1998, rev. SB 50 41'20'' 15 48'00'' 870 m 36 apomictic 434 IS CZ, Krkonoše Mts, Benecko, FK, AK & JC 2000, rev. SB 50 40'10'' 15 33'00'' 800 m 36 NS 521 IS CZ, Krkonoše Mts, Rýchory - Sklenářovice, FK 2001, rev. SB 50 38'25'' 15 51'10'' 800 m 36 NS ise Gr 2 PL, Góry Izerskie Mts, between Orle and former settlement Gross Iser, SB 1999 50 49'42'' 15 22'30'' 810 m 36 NS ise Hot 1 D, Erzgebirge Mts, Schellerhau - Hotel, SB 1999 50 46'38'' 13 41'24'' 770 m NC NS ise Neu 12 D, Erzgebirge Mts, Neuwernsdorf, SB 2000 50 42'07'' 13 31'17'' 600 m 36 NS H. leptophyton Nägeli et Peter, 2n = 7x 597 LEP CZ, Roudnice, Přestavlky, FK 2002 50 24'10'' 14 11'25'' 210 m 63 NS H. macrostolonum G. Schneider, 2n = 6x 8

927 MA SK, Slovenský raj Mts, Kopanisko - lúky, HWS 2004, rev. FS 48 54'00'' 20 17'35'' 980 m NC NS H. piloselliflorum Nägeli et Peter, 2n = 4x 227/1 PF CZ, Krkonoše Mts, Dolní Malá Úpa, FK, AK, SB & JC 1999 50 43'20'' 15 48'40'' 950 m 36 b apomictic b 238 PF CZ, Krkonoše Mts, Slezské sedlo near Špindlerovka, FK, AK, SB & JC 1999 50 45'50'' 15 37'20'' 1200 m 36 b apomictic 431 AP CZ, Krkonoše Mts, Horní Mísečky, FK, AK & JC 2000, rev. SB 50 44'05'' 15 34'10'' 1020 m 36 NS 736 PF CZ, Šumava Mts, Jelení Vrchy, FP, FK, SB & FS 2002 48 48'55'' 13 52'45'' 890 m NC sexual 742 PF CZ, Šumava Mts, Hadí vrch near Hartmanice, FP, FK, SB & FS 2002 49 11'05'' 13 20'45'' 960 m NC sexual 751 PF CZ, Šumava Mts, Stará Hůrka, FP, FK, SB & FS 2002 49 07'30'' 13 19'48'' 1010 m NC sexual 752 PF CZ, Šumava Mts, Slučí Tah near Hartmanice, FP, FK, SB & FS 2002 49 09'10'' 13 21'00'' 880 m NC sexual 552 SC CZ, Šumava Mts, Slučí Tah near Hartmanice, FP & FK 2001, rev. SB 49 09'10'' 13 21.00'' 880 m 36 sexual H. piloselliflorum Nägeli et Peter, 2n = 5x 184/3 PF CZ, Kralický Sněžník Mts, Dolní Morava, FK & AK 1999 50 07'50'' 16 48'10'' 620 m 45 c NS 213 PF CZ, Krkonoše Mts, Horní Malá Úpa - Pomezní Boudy, FK, AK, SB & JC 1999 50 44'30'' 15 48'40'' 1020 m 45 b NS 440 AP CZ, Krkonoše Mts, Horní Malá Úpa - Pomezní Boudy, FK, AK & JC 2000, rev. SB 50 44'20'' 15 48'50'' 1000 m 45 apomictic 370 PF PL, Góry Izerskie Mts, Velká Jizerská louka meadow close to Czech-Polish border, FK 2000, 50 51'00'' 15 21'40'' 830 m 45 d NS rev. HWS 821 PI-FL PL, Jerzmanowice near Kraków, Lysa skala, OR 2000 50 13'30'' 19 45'30'' 450 m NC NS H. piloselliflorum Nägeli et Peter, 2n = 6x 224/1 PF CZ, Krkonoše Mts, Dolní Malá Úpa - Rennerovy Boudy, FK, AK, SB & JC 1999 50 43'40'' 15 49'20'' 1060 m 54 b apomictic b 230/2 PF CZ, Krkonoše Mts, Dolní Malá Úpa, between the church and Spálený Mlýn, FK, AK, SB & JC 50 42'50'' 15 48'50'' 880 m 54 b apomictic b 1999 108 AP CZ, Krkonoše Mts, Horní Malá Úpa - Pomezní Boudy, FK 1997, rev. SB 50 44'20'' 15 48'50'' 1000 m 54 a apomictic a H. rothianum Wallr., 2n = 4x 10 (Boří les) CZ, Břeclav-Poštorná, near the railway station Boří les, TP, JC & JŠ 1998 48 44 15 16 51 58 160 m 36 c apomictic 9 (Bzenec) CZ, near railway-station Bzenec-Přívoz near Bzenec, TP 1998 48 55 47 17 16 52 180 m 36 c apomictic 36 (Děvín) CZ, Pavlovské kopce hills, Děvín hill, TP 1999 48 52 06 16 38 54 540 m 36 c apomictic 43 (Habří) CZ, České středohoří Mts, Habří, Rač hill, TP 1999 50 37 06 13 55 43 250 m NC apomictic 70 (Havraníky) CZ, Havranické vřesoviště heathland near Havraníky, TP & JC 2001 48 48 40 15 59 45 300 m NC apomictic 66 (Skalky) CZ, Skalky hill near Havraníky, TP 2001 48 48 18 16 00 31 330 m NC apomictic 45 (Trojhora) CZ, České středohoří Mts, Chudoslavice, Trojhora hill, TP 2002 50 35 24 14 11 28 430 m 36 c apomictic H. rubrum Peter, 2n = 6x 549 RU CZ, Šumava Mts, Slučí Tah near Hartmanice, FP & FK 2001, rev. SB & FS 49 09'10'' 13 21'00'' 880 m 54 apomictic 756 RU CZ, Šumava Mts, Zhůří near Železná Ruda, FP, FK, SB & FS 2002 49 10'50'' 13 20'20'' 950 m NC apomictic 8/3 RU CZ, Krkonoše Mts, Velká Úpa - Janovy Boudy, FK & AK 1998, rev. SB 50 41'20'' 15 48'00'' 860 m 54 b apomictic b 732 SF CZ, Krkonoše Mts, Velká Úpa - Janovy Boudy, FK 2002, rev. SB 50 41'30'' 15 47'50'' 880 m 54 NS 97 RU CZ, Krkonoše Mts, Horní Malá Úpa - Pomezní Boudy, FK 1997, rev. SB 50 44'20'' 15 48'50'' 1000 m 54 a apomictic a 9

C2 RU CZ, Krkonoše Mts, Horní Malá Úpa - Černá Voda, JC & VK 2000 50 44'15'' 15 49'20'' 1050 m NC NS H. scandinavicum Dahlst., 2n = 4x 910 GL CZ, Šumava Mts, Gerlova Huť, FK 2003, rev. SB 49 09'54'' 13 16'44'' 950 m NC NS 913 CE CZ, Šumava Mts, Gerlova Huť, FK 2003, rev. SB 49 09'54'' 13 16'44'' 950 m NC apomictic H. schultesii F.W. Schultz, 2n = 3x 735 SC CZ, Šumava Mts, Jelení Vrchy, FP, FK, SB & FS 2002 48 48'55'' 13 52'45'' 890 m NC sterile 763/1 SC CZ, Šumava Mts, Horská Kvilda, FP, FK, SB & FS 2002 49 03'35'' 13 33'40'' 1130 m NC sterile H. schultesii F.W. Schultz, 2n = 4x 8/11 SC CZ, Krkonoše Mts, Velká Úpa - Janovy Boudy, FK & AK 1998, rev. SB 50 41'20'' 15 48'00'' 860 m 36 apomictic 188 LAxPI CZ, Krkonoše Mts, Pec pod Sněžkou, FK 1999, rev. SB 50 41'50'' 15 43'50'' 830 m 36 b NS 788 SC CZ, Českomoravská vrchovina Highlands, Cikháj near Žďár nad Sázavou, OR 2001 49 39'07'' 15 57'54'' 650 m 36 d NS 738 SC CZ, Šumava Mts, Jelení Vrchy, FP, FK, SB & FS 2002 48 48'55'' 13 52'45'' 890 m NC sexual 923 SC SK, Slovenský raj Mts, Stratená, meadow area Hanesová, HWS 2004, rev. SB 48 51'15'' 20 18'25'' 910 m NC NS 928 SC SK, Slovenský raj Mts, Stratená, FS 2004 48 52'20'' 20 20'40'' 870 m NC NS H. schultesii F.W. Schultz, 2n = 5x 118 SC CZ, Krkonoše Mts, Horní Mísečky, FK & AK 1997, rev. HWS & SB 50 44'10'' 15 34'10'' 1060 m 45 a apomictic a 258/1 SC SK, Velká Fatra Mts, Králova Studňa Mt, FK & AK 1999, rev. HWS 48 52'40'' 19 02'30'' 1270 m 45 c NS 528 SC PL, Massife of Śłęza near Sobótka, PK 2001 50 50'40'' 16 43'30'' 290 m NC NS H. stoloniflorum Waldst. et Kit., 2n = 4x 534 SF CZ, Šumava Mts, Zhůří near Kašperské Hory, FP & FK 2001, rev. SB & FS 49 05'10'' 13 33'20'' 1120 m 36 sexual 755 SF CZ, Šumava Mts, Zhůří near Železná Ruda, FP, FK, SB & FS 2002 49 10'50'' 13 20'20'' 950 m NC sexual 835/2 SF D, North Rhine Westfalia, Hagen - Eilpe, Krähnocken, above Eilper Hangstieg, UR 2003 51 21' 7 28' 260 m NC sexual Recent hybrids H. aurantiacum H. lactucella, 2n = 3x 818 AuxLA CZ, Vimperk, FP 2002, rev. SB & FS 49 03'20'' 13 46'30'' 700 m 27 NS H. aurantiacum H. rubrum, 2n = 7x 433 AU CZ, Krkonoše Mts, Horní Mísečky, FK, AK & JC 2000 50 44'10'' 15 34'00'' 1030 m 63 apomictic H. floribundum H. pilosella, 2n = 6x 743 IS CZ, Šumava Mts, Hadí vrch near Hartmanice, FP, FK, SB & FS 2002 49 11'05'' 13 20'45'' 960 m 54 apomictic 823 FL CZ, Českomoravská vrchovina Highlands, Horní Bory near Velké Meziříčí, OR 2001 49 25'20'' 16 01'10'' 540 m 54 NS H. lactucella H. onegense, 2n = 2x 6/6 SP4 CZ, Krkonoše Mts, Velká Úpa - Janovy Boudy, FK & AK 1998, rev. SB 50 41'20'' 15 47'40'' 870 m 18 b sexual b 924 LaxON SK, Slovenský raj Mts, Stratená, meadow area Hanesová, HWS 2004 48 51'15'' 20 18'25'' 910 m NC NS H. pilosella H. rubrum, 2n = 5x 10

220 SF CZ, Krkonoše Mts, Horní Malá Úpa - Pomezní Boudy, FK, SB & JC 1999 50 44'10'' 15 48'00'' 950 m 45 b apomictic *Country abbreviations: A Austria; BG Bulgaria; CZ Czech Republic; D Germany; H Hungary; PL Poland; RO Romania; SK Slovak Republic. Collector and/or revisor abbreviations: AK A. Krahulcová; DH D. Hrčka; FK F. Krahulec; FP F. Procházka; FS F. Schuhwerk; HWS participants of the Hieracium Workshop; JC J. Chrtek; JD J. Danihelka; JH J. Hadinec; JŠ J. Škorničková; MC M. Chytrý; OR O. Rotreklová; PK P. Kwiatkowski; PM P. Mráz; RŠ R. Šimek; SB S. Bräutigam; TP T. Peckert; UR U. Raabe; VK V. Košťálová; VM V. Mahelka; VV V. Vladimirov; ZS Z. Szeląg. Chromosome numbers were determined from the same individual as subjected to flow cytometric analyses, except for counts done by R. Šimek and V. Vladimirov that are based on different individual from the same locality. Published counts: a Krahulcová and Krahulec, 1999; b Krahulcová et al., 2001; c Rotreklová et al., 2002; d Rotreklová et al., 2005. Unless otherwise indicated, other chromosome numbers were determined by A. Krahulcová. NC = chromosomes not counted. Breeding systems of the plants published (see references a-d above) or under investigation (A. Krahulcová et al., unpubl. res.). 11