Electronic Appendix 1. List of localities used for cytometric analysis.

Kubešová M., Moravcová L., Suda J., Jarošík V. & Pyšek P. (2010): Naturalized plants have smaller genomes than their non-invading relatives: a flow cytometric analysis of the Czech alien flora. Preslia 82: 81 96. Electronic Appendix 1. List of localities used for cytometric analysis. Species Locality District Locality description Latitude Longitute Abutilon theophrasti Med. Ovčáry Kolín sugar beet field, 0.8 km NE of church Ovčáry 50 03'54.4'' 15 15'10.9'' Abutilon theophrasti Med. Horky Kutná Hora sugar beet field, 0.1km E of the railway station Horky 49 52'17.3'' 15 26'40.0'' Abutilon theophrasti Med. Sendražice Kolín field in the NE edge of the Sendražice village 50 03'00.1'' 15 13'17.4'' Amaranthus albus L. Týniště nad Orlicí Rychnov nad Kněžnou railway station Týniště nad Orlicí 50 09'40.5'' 16 04'03.4'' Amaranthus albus L. Liteň Beroun railway station Liteň 49 54'12.6'' 14 09'30.4'' Amaranthus albus L. Klentnice Břeclav hunting cottage in the forest clearing, 2 km N of Klentnice church 48 50'17.4'' 16 41'09.9'' Amaranthus albus L. Hrušky Břeclav sandpit bank, 1.2 km WSW of the railway station Hrušky 48 46'34.7'' 16 55'34.7'' Amaranthus blitoides S. Watson Hrušky Břeclav sandpit bank, 1.2 km WSW of the railway station Hrušky 48 46'36.6'' 16 55'31.9'' Amaranthus powellii S. Watson Břeclav Břeclav field edge close to the parking place, 1.2 km SSE of the railway station Břeclav 48 44'35.8'' 16 53'54.4'' Amaranthus powellii S. Watson Dobřichovice Praha-západ ruderal site near the Berounka river in the village 49 55'46.3'' 14 17'15.1'' Amaranthus powellii S. Watson Bolehošť Rychnov nad Kněžnou ruderal site in the village 50 12'19.3'' 16 04'44.1'' Amaranthus powellii S. Watson Všenory Praha-západ ruderal site at the railway station Všenory 49 55'54.4'' 14 18'08.4'' Amaranthus retroflexus L. Domanice Strakonice field edge at the W edge of the village 49 17'47.7'' 13 55'05.7' Amaranthus retroflexus L. Břeclav Břeclav field edge close to the parking place, 1.2 km SSE of the railway station Břeclav 48 44'35.8'' 16 53'54.4'' Amaranthus retroflexus L. Bolehošť Rychnov nad Kněžnou ruderal site in the village 50 12'19.3'' 16 04'44.1'' Amaranthus retroflexus L. Praha-Braník Praha railway station Praha-Braník 50 01'43.0'' 14 24'20.0'' Amaranthus retroflexus L. Hrušky Břeclav sandpit bank, 1.2 km WSW of the railway station Hrušky 48 46'34.7'' 16 55'34.7'' Amaranthus retroflexus L. Liteň Beroun railway station Liteň 49 54'12.6'' 14 09'30.4'' Ambrosia artemisiifolia L. Břeclav Břeclav field edge close to the parking place, 1.2 km SSE of the railway station Břeclav 48 44'32.8'' 16 53'49.8'' Ambrosia artemisiifolia L. Lanžhot Břeclav ruderal site close to soil waste site, 1.4 km SE of the village 48 43'35.0'' 16 58'42.7'' Ambrosia artemisiifolia L. Pečky Kolín railway station Pečky 50 05'29.0'' 15 01'14.1'' Ambrosia psilostachya DC. Čelákovice Praha-východ ruderal site close to the railway, 0.4 km NNE of railway station Čelákovice-Jiřina 50 10'18.9'' 14 46'09.9'' Ambrosia trifida L. Velký Osek Kolín field, 1.3 km SW of the railway station Velký Osek 50 05'37.4'' 15 10'47.9'' Angelica archangelica L. Lysá nad Labem Nymburk Labe river bank,1.9 km SW of the railway station Lysá nad Labem 50 10'46.2'' 14 49'50.5'' Angelica archangelica L. Straky Nymburk Vlkava river bank, 2.7 km WNW of the railway station Straky 50 13'56.4'' 14 57'08.8'' Angelica archangelica L. Velké Březno Ústí nad Labem Labe river bank, 1 km NE of the railway station Velké Březno 50 40'02.7'' 14 09'36.2'' Angelica archangelica L. Velké Žernoseky Litoměřice Labe river bank bank, 0.9 km NW of the Velké Žernoseky church 50 32'43.1'' 14 03'24.7'' Angelica archangelica L. Čelákovice Praha-východ Labe river bank, 1 km NNW of the railway station Čelákovice 50 10'03.5'' 14 45'08.4'' Antirrhinum majus L. Mikulov Břeclav walls of the Mikulov castle 48 48'25.8'' 16 38'13.2'' 1

Antirrhinum majus L. Štramberk Nový Jičín walls of the Štramberk houses in the village 49 35'15.6'' 18 07'22.4'' Arabis alpina L. Mikulov Břeclav walls of the Mikulov castle 48 48'22.6'' 16 38'09.2'' Arabis alpina L. Velhartice Klatovy walls of the Velhartice castle 49 15'46.9'' 13 23'59.0'' Asclepias syriaca L. Břeclav Břeclav meadow close to Pohansko castle 48 43'50.6'' 16 53'45.6' Asclepias syriaca L. Břeclav Břeclav road bank close to Lány castle 48 42'58.1'' 16 55'16.3'' Asclepias syriaca L. Břeclav-Poštorná Břeclav road bank, 0.5 km WNW of the railway station Boří les 48 44'19.5'' 16 51'44.6' Aster lanceolatus Willd. Hustopeče Břeclav road bank, 1.9 km SE of the railway station Hustopeče 48 55'47.9'' 16 45'20.8'' Aster lanceolatus Willd. Valtice-Úvaly Břeclav road bank at the E edge of the Úvaly village 48 44'28.8'' 16 42'13.0'' Aster lanceolatus Willd. Lanžhot Břeclav road bank, 1.8 km NW of the railway station Lanžhot 48 44'24.3'' 16 56'23.2'' Bidens connata Willd. Všestudy Mělník wetland near Všestudský potok brook, 0.5 km SSE of the Všestudy village municipality 50 17'13.4'' 14 20'33.2'' Bidens connata Willd. Brozánky-Hořín Mělník wetland at the Bažantnice Brozánky pheasantry, 1.4 km NW of the Hořín castle 50 21'05.9'' 14 26'41.6'' Bidens frondosa L. Lanžhot Břeclav forest clearing, 12.5 km SSW of the railway station Lanžhot 48 37'27.1'' 16 55'53.6'' Bidens frondosa L. Všenory Praha-západ Berounka river bank, 1.6 km NE of the railway station Všenory 49 56'21.0'' 14 19'27.4'' Bidens frondosa L. Velké Březno Ústí nad Labem Labe river bank, 1 km NE of the railway station Velké Březno 50 40'02.7" 14 09'36.2" Bidens frondosa L. Vilsnice Děčín ruderal site, 0.9 km S of railway station Vilsnice 50 44'15.1'' 14 10'53.0'' Bidens frondosa L. Kostice Břeclav Morava river bank, 3.5 km ESE of the railway station Lanžhot 48 43'52.4'' 17 00'26.0'' Bidens frondosa L. Praha-Komořany Praha Vltava river bank, 0.6 km SSW of railway station of Praha-Komořany 49 59'09.5'' 14 24'03.0'' Bunias orientalis L. Beroun-Jarov Beroun road bank, 1.7 km SSW of the railway station Beroun 49 56'33.9'' 14 04'03.2'' Bunias orientalis L. Drhovy Příbram road bank and field edge, 0.7 km NW of the Drhovy village municipality 49 44'44.3'' 14 13'39.6'' Bunias orientalis L. Hostinné Hostinné road bank, 0.3 km W of railway station Prosečné 50 32'54.4'' 15 41'28.2'' Cannabis ruderalis Janisch. Čejkovice Hodonín field edge, 3.2 km ENE of the Čejkovice church 48 54'27.7'' 16 59'06.1'' Cannabis ruderalis Janisch. Sedlec Břeclav field edge, 1.8 km NNE of the railway station Sedlec 48 47'22.7'' 16 42'26.6'' Cardamine chelidonia L. Domanice Strakonice forest along the railway, 0.3 m SSW of the railway station Domanice 49 17'55.9'' 13 54'49.1'' Cardamine chelidonia L. Hněvkov Strakonice forest edge along the railway, 2.1 km ESE of the railway station Mačkov 49 23'39.8'' 13 54'53.9'' Cardamine chelidonia L. Veselí nad Lužnicí Tábor road bank, 2.2 km SSW of the railway station Veselí nad Lužnicí 49 10'26.7'' 14 43'12.3'' Cardamine chelidonia L. Bludov Šumperk forest road, 2.3 km NNE of the Bludov church 49 57'40.3'' 16 56'38.6'' Claytonia alsinoides Sims Průhonice Praha-západ forest in the Park Průhonice 49 59'51.9'' 14 33'40.6'' Collomia grandiflora Lindl. Nižbor Beroun forest edge, 1.5 km SSW of the railway station Nižbor 49 59'23.5" 13 59'43.0'' Consolida orientalis (Gr. et Godr.) Schrödinger Bořanovice Praha-východ field edge, 1 km SSW NE of the Líbeznice church 50 10'56.5'' 14 29'18.6'' Consolida orientalis (Gr. et Godr.) Schrödinger Trubín Beroun field edge, 1.1 km ESE of the Trubín village municipality 49 56'28.3'' 14 01'00.1'' Consolida orientalis (Gr. et Godr.) Schrödinger Lochkov Praha field edge, 0.5 km WNW of the Lochkov castle 50 00'11.8'' 14 21'40.0'' Consolida orientalis (Gr. et Godr.) Schrödinger Slivenec Praha field edge, 0.5 km WNW of the Slivenec church 50 01'12.7" 14 20'51.8" Conyza canadensis (L.) Cronq. Dubňany Hodonín forest clearing near former Důl Osvobození mine 48 54'47.1'' 17 02'51.0'' Conyza canadensis (L.) Cronq. Pečky Kolín railway station Pečky 50 05'28.9'' 15 07'41.5'' Conyza canadensis (L.) Cronq. Praha-Modřany Praha road edge, 0.5 km S of the railway station Praha-Modřany 49 59'53.7" 14 24'13.4" Conyza canadensis (L.) Cronq. Mutěnice Hodonín ruderal site, 0.4 km ESE of the railway station Mutěnice 48 54'16.8'' 17 02'51.0'' 2

Corydalis lutea (L.) DC. Průhonice Praha-západ dwarf wall in the Park Průhonice 50 00'02.1'' 14 33'24.6'' Corydalis lutea (L.) DC. Masákova Lhota Prachatice walls of the houses in the village 49 05'11.6'' 13 40'49.5'' Corydalis lutea (L.) DC. Všenory Praha-západ wall of the house in the village 49 55'18.5'' 14 18'28.9'' Cuscuta campestris Yuncker Libice nad Cidlinou Nymburk road bank, 0.3 km ENE of the railway station Libice nad Cidlinou 50 07'40.7'' 15 11'17.3'' Cuscuta campestris Yuncker Šeřeč-Domkov Náchod reservoir bank, 4.2 km NW of the railway station Nové Město nad Metují 50 22'52.4" 16 05'48.3" Cuscuta campestris Yuncker Doubravice Náchod reservoir bank, 3.4 km NW of the railway station Nové Město nad Metují 50 22'22.0" 16 05'50.8" Datura stramonium L. Praha-Modřany Praha ruderal site, 0.3 km SE of the railway station Praha-Modřany 50 00'13.9'' 14 24'17.7'' Datura stramonium L. Sedlec Břeclav field edge, 0.8 km SE of the railway station Sedlec 48 46'16.8'' 16 42'33.7'' Datura stramonium L. Lanžhot Břeclav waste site of the soil, 1.3 km SE of the railway station Lanžhot 48 43'35.0'' 16 58'41.9'' Datura stramonium L. Dobřichovice Praha-západ river bank, 0.2 km N of the railway station Dobřichovice 49 55'46.3'' 14 17'15.1'' Digitalis purpurea L. Buková u Příbramě Příbram edge of the lom Kuchyňka quarry, 3.6 km NNW of Buková u Příbramě church 49 47'06.6'' 14 04'14.9'' Digitalis purpurea L. Smolné Pece Karlovy Vary edge of the meadow, 0.8 km ENE of the railway station Nová Rolezastávka 50 17'33.4'' 12 45'59.2'' Digitalis purpurea L. Hranice Cheb forest edge, 3.3 km NW of the railway station Hranice 50 18'51.0'' 12 08'23.0'' Duchesnea indica (Andrew) Focke Roztoky Praha-západ road bank, 0.3 km NW of the railway station Roztoky 50 09'40.3'' 14 23'42.4'' Duchesnea indica (Andrew) Focke Praha-Karlov Praha road edge, Albertov street 50 04'11.1'' 14 25'36.7'' Duchesnea indica (Andrew) Focke Průhonice Praha-západ Park Průhonice 50 00'03.2'' 14 33'24.0'' Echinocystis lobata (Michx.) Torr. et Gray Všenory Praha-západ Vltava river bank, 1.6 km ENE of the railway station Všenory 49 56'18.7'' 14 19'33.4'' Echinocystis lobata (Michx.) Torr. et Gray Stráž nad Ohří Karlovy Vary Ohře river bank, 0.4 km WSW of the railway station Stráž nad Ohří 50 20'21.0'' 13 03'12.5'' Echinocystis lobata (Michx.) Torr. et Gray Lanžhot Břeclav wire fence on the road edge, 12 km SSW of Lanžhot village 48 46'12.5'' 17 03'17.3'' Echinocystis lobata (Michx.) Torr. et Gray Lanžhot Břeclav Morava river bank, 5.2 km WSW of the railway station Lanžhot 48 44'44.2'' 17 01'36.0'' Echinops sphaerocephalus L. Velký Osek Kolín road edge, 1.3 km SW of the railway station Velký Osek 50 05'38.0'' 15 10'48.9'' Echinops sphaerocephalus L. Kadaň Chomutov road bank, 5.6 km ENE of the railway station Kadaň 50 22'44.3'' 13 21'20.7'' Echinops sphaerocephalus L. Duchcov Teplice road bank, 2 km N of the railway station Duchcov 50 37'01.2'' 13 43'41.7'' Echinops sphaerocephalus L. Nový Bydžov Hradec Králové ruderal site, 2.5 km E of the railway station Nový Bydžov 50 14'50.1'' 15 30'52.6'' Epilobium ciliatum Rafin. Hvožďany Příbram meadow, 1.4 km WSW of the Hvožďany church 49 31'15.2'' 13 47'15.6'' Epilobium ciliatum Rafin. Pec pod Sněžkou Trutnov ruderal site near the chalet, 1.5 km W of the Pec pod Sněžkou village 50 41'38.2'' 15 42'21.7'' Epilobium ciliatum Rafin. Řepešín-Záblatí Prachatice wet meadow, 1.2 km NNW of the Řepešín church 49 00'24.1" 13 53'42.5" Epilobium dodonaei Vill. Karviná Karviná spoil ground, 3 km SSW of the railway station Karviná 49 50'53.2" 18 30'06.3" Epilobium dodonaei Vill. Koněprusy Beroun limestone quarry Čertovy schody, 0.8 km SSE of Koněprusy village 49 54'47.0'' 14 03'59.4'' Epilobium dodonaei Vill. Štramberk Nový Jičín limestone quarry Kamenárka, 0.7 km NW of the railway station Štramberk 49 35'17.4'' 18 07'32.9'' Erigeron annuus (L.) Pers. Průhonice Praha-západ ruderal site, 0.9 km SE of the Průhonice castel 49 59'45.2'' 14 34'02.4'' Erigeron annuus (L.) Pers. Hořice Jičín former sandpit, 3.5 km WNW of the railway station Hořice 50 23'15.2'' 15 37'46.1'' Erigeron annuus (L.) Pers. Vilsnice Děčín ruderal site, 0.9 km S of the railway station Vilsnice 50 44'15.1'' 14 10'53.0'' Erigeron annuus (L.) Pers. Roztoky Praha-západ ruderal site, 0.5 km SSW of the railway station Roztoky 50 09'27.5'' 14 23'47.4'' Erigeron annuus (L.) Pers. Týniště nad Orlicí Rychnov nad Kněžnou railway station, Týniště nad Orlicí 50 09'37.8" 16 04'02.6" 3

Erigeron annuus (L.) Pers. Praha-Modřany Praha railway embankment close to the railway station Praha-Modřany 49 59'36.4" 14 24'17.2" Erigeron annuus (L.) Pers. Velký Osek Kolín field, 1.2 km SW of of the railway station Velký Osek 50 05'36.5'' 15 10'51.9'' Erucastrum gallicum (Willd.) O. E. Schulz Lysá nad Labem Nymburk field, 2.9 km NNW of the railway station Lysá nad Labem 50 12'54.1'' 14 49'30.3'' Galega officinalis L. Velký Osek Kolín road edge, 3.5 km NW of the railway station Velký Osek 50 07'03.4'' 15 09'03.8'' Galega officinalis L. Břeclav Břeclav road edge, 1.4 km SSE of the railway station Břeclav 48 44'23.5" 16 53'50.6" Galega officinalis L. Pavlov Břeclav road bank close to Novomlýnské nádrže reservoir, 1.3 km ESE of the Pavlov church 48 52'19.5'' 16 41'26.1'' Galega officinalis L. Průhonice Praha-západ ruderal road edge in the village 50 00'20.0'' 14 33'56.0'' Galinsoga quadriradiata Ruiz et Pavón Strakonice Strakonice railway station Strakonice 49 59'31.5'' 14 24'06.6'' Galinsoga quadriradiata Ruiz et Pavón Praha-Komořany Praha ruderal site, 0.3 km NW of the railway station Praha-Komořany 49 59'31.7'' 14 24'06.3'' Galinsoga quadriradiata Ruiz et Pavón Smolné pece Karlovy Vary ruderal site, 0.8 ENE of the railway station Nová Role-zastávka 50 17'33.2'' 12 46'01.6'' Galinsoga parviflora Cav. Lysá nad Labem Nymburk field edge, 2.3 km NW of the railway station Lysá nad Labem 50 12'56.9'' 14 49'35.4'' Galinsoga parviflora Cav. Lanžhot Břeclav field edge, 1.2 km SW of the railway station Lanžhot 48 43'33.8'' 16 56'56.7'' Galinsoga parviflora Cav. Řepešín- Záblatí Prachatice pasture at the W edge of the Řepešín u Záblatí village 49 00'08.2" 13 55'34.2 Geranium pyrenaicum Burm. fil. Průhonice Praha-západ road edge in the Park Průhonice 49 59'59.7'' 14 33'33.0'' Geranium pyrenaicum Burm. fil. Droužetice Strakonice road edge in the village 49 17'34.2'' 13 54'53.3'' Geranium pyrenaicum Burm. fil. Lednice Břeclav road edge, 0.3 m WSW of the railway station Lednice 48 47'38.8'' 16 47'55.7'' Geranium pyrenaicum Burm. fil. Praha-Modřany Praha road edge, 1.5 km ESE of the railway station Praha-Modřany 50 00'03.8" 14 25'26.0" Helianthus tuberosus L. Všenory Praha-západ field edge, 1.6 km ENE of the railway station Všenory 49 56'17.8'' 14 19'34.3'' Helianthus tuberosus L. Hustopeče Břeclav road bank, 1.5 km SE of the railway station Hustopeče 48 55'47.9'' 16 45'20.8'' Helianthus tuberosus L. Lanžhot Břeclav field edge, 2.4 km NW of the railway station Lanžhot 48 44'23.9'' 16 56'26.2'' Heracleum mantegazzianum Sommier et Levier Mníšek pod Brdy Praha-západ ruderal site, 3.4 km NNE of the railway station Mníšek pod Brdy 49 53'02.4'' 14 16'47.7'' Heracleum mantegazzianum Sommier et Levier Lazy-Kynžvart Cheb meadow, 7.5 km NNE of the railway station Kynžvart 50 03'45.2'' 12 37'34.1'' Heracleum mantegazzianum Sommier et Levier Rovná Sokolov meadow, 11.8 km NNW of the railway station Kynžvart 50 05'58.9'' 12 34'08.2'' Heracleum mantegazzianum Sommier et Levier Nová Ves po Pleší Praha-západ forest clearing, 1.5 km NNE of the railway station Nová Ves pod Pleší 49 50'21.6" 14 16'43.7 Hesperis matronalis L. Štěpánov Teplice road bank, 2.2 km WSW of the Štěpánov village 50 31'34.9'' 13 50'10.0'' Hesperis matronalis L. Průhonice Praha-západ forest edge at the Park Průhonice 49 59'43.4'' 14 33'50.5'' Hesperis matronalis L. Praha-Komořany Praha road bank, 1.2 km NNE of the railway station Praha-Zbraslav 49 58'53.0'' 14 24'16.4'' Hordeum jubatum L. Zlatá Praha-východ road edge in the village 50 01'51.6'' 14 40'18.8'' Hordeum jubatum L. Praha-Uhříněvěs Praha ruderal site, 0.5 km SSE of the railway station Praha-Uhříněves 50 01'46.3" 14 35'39.7" Hordeum jubatum L. Praha-Kobylisy Praha road edge, 0.2 km NW of the Kobylisy-vozovna tram station 50 07'44.8" 14 31'21.3" Chenopodium pumilio R. Br. Břeclav-Poštorná Břeclav pine forest, 1.3 km W of the railway station Břeclav-Poštorná 48 45'06.6'' 16 50'08.0'' Chenopodium strictum Roth Pečky Kolín railway station Pečky 50 05'30.2'' 15 01'10.0'' Chenopodium strictum Roth Břeclav-Poštorná Břeclav railway station Břeclav-Poštorná 48 44'16.7'' 16 52'23.7'' Chenopodium strictum Roth Domanice Strakonice ruderal site in the village 49 17'49.4'' 13 55'10.6'' Impatiens glandulifera Royle Praha-Modřany Praha Vltava river bank, 0.6 km SSW of the railway station Praha- Komořany 49 59'08.7'' 14 24'02.9'' Impatiens glandulifera Royle Čelina Příbram pond bank, 0.7 km NW of the Čelina castle 49 44'05.7'' 14 19'13.6'' 4

Impatiens glandulifera Royle Stráž nad Ohří Karlovy Vary Ohře river bank, 0.8 km SW of the railway station Stráž nad Ohří 50 04'15.4" 16 19'26.4" Impatiens glandulifera Royle Potštejn Rychnov nad Kněžnou Divoká Orlice river bank, 1.4 km SSE of the railway station Potštejn 50 07'44.8" 14 31'21.3" Impatiens parviflora DC. Černolice Praha-západ forest, 1.7 km SSE of the railway station Dobřichovice 49 54'48.7" 14 18'04.3" Impatiens parviflora DC. Praha-Zbraslav Praha forest, 0.4 km NE of the railway station Praha-Zbraslav 49 58'09.9" 14 24'47.2" Impatiens parviflora DC. Čelina Příbram Čelinský potok brook bank, 0.6 km NW of the Čelina castle 49 44'04.6'' 14 19'13.9'' Impatiens parviflora DC. Potštejn Rychnov nad Kněžnou forest, 1.5 km SSE of the railway station Potštejn 50 04'15.4" 16 19'26.4" Impatiens parviflora DC. Strakonice Strakonice railway embankment, 0.2 km S of the Strakonice castle 49 15'22.3'' 13 54'01.7'' Imperatoria ostruthium L. Pec pod Sněžkou Trutnov ruderal site near the chalet, 1.5 km W of Pec pod Sněžkou village 50 41'38.2'' 15 42'21.7'' Imperatoria ostruthium L. Jizerka-Kořenov Jablonec nad Nisou road edge, 5.7 km NNW of the railway station Kořenov 50 48'69.1'' 15 21'13.2'' Imperatoria ostruthium L. Jizerka-Kořenov Jablonec nad Nisou meadow, near the chalet, 4.8 km NNE of the railway station Kořenov 50 48'41.5'' 15 21''07.9'' Imperatoria ostruthium L. Horní Blatná Karlovy Vary meadow, 3.5 km ENE of the railway station Horní Blatná 50 23'29.7" 12 48'57.5" Imperatoria ostruthium L. Smržovka Jablonec nad Nisou meadow, 0.8 km SE of the railway station Smržovka 50 44'10.2" 15 15'08.8" Inula helenium L. Domanice u Strakonic Strakonice pond bank, 0.8 km S of the railway station Domanice 49 17'34.1'' 13 54'53.3'' Iva xanthiifolia Nutt. Týniště nad Orlicí Rychnov nad Kněžnou railway station Týniště nad Orlicí 50 09'40.5'' 16 04'03.4'' Iva xanthiifolia Nutt. Beroun Beroun railway station Beroun 49 57'23.4'' 14 04'22.3'' Juncus tenuis Willd. Pavlov Břeclav road bank close to Novomlýnské nádrže reservoir, 0.9 km ESE of the Pavlov church 48 52'22.0" 16 41'00.2" Juncus tenuis Willd. Koněprusy Beroun limestone quarry Kobyla, 1.4 km SE of the Koněprusy church 49 54'39.2'' 14 04'35.7'' Juncus tenuis Willd. Čelina Příbram ruderal site near the former štola Josef mine adit 49 43'48.5" 14 20'54.6" Juncus tenuis Willd. Chlístov-Kratušín Prachatice on the road, 1.5 km WSW of the Kratušín church 49 00'47.4" 13 54'34.0 Kochia scoparia (L.) Schrader Mikulov Břeclav railway station Mikulov 48 48'00.3'' 16 37'43.7'' Kochia scoparia (L.) Schrader Pečky Kolín railway station Pečky 50 05'28.9'' 15 01'15.5'' Kochia scoparia (L.) Schrader Praha-Radlice Praha road edge of the Strakonická street, 1.1 km SSE of the railway station Praha-Smíchov 50 03'4.9'' 14 24'37.7'' Lepidium densiflorum Schrader Strakonice Strakonice railway station Strakonice 49 59'31.5'' 14 24'06.6'' Lepidium densiflorum Schrader Praha-Braník Praha railway station Praha-Braník 50 01'44.5'' 14 24'28.6'' Lepidium densiflorum Schrader Pečky Kolín railway station Pečky 50 05'28.4'' 15 01'19.0'' Lupinus polyphyllus Lindl. Koněprusy Beroun limestone quarry Kobyla, 1.4 km SE of the Koněprusy church 49 55'04.0" 14 02'04.6" Lupinus polyphyllus Lindl. Pozorka-Nejdek Karlovy Vary road edge at the village Pozorka 50 18'44.9" 12 45'42.0" Lupinus polyphyllus Lindl. Hranice Cheb lawn in the village Hranice 50 11'41.0'' 12 12'59.0'' Lupinus polyphyllus Lindl. Smolné Pece Karlovy Vary ruderal site, 0.8 ENE of the railway station Nová Role-zastávka 50 17'34.7'' 12 45'59.8'' Lupinus polyphyllus Lindl. Borová Lada Prachatice road edge, 5.2 km SSW of the Borová Lada village 48 57'05.2'' 13 38'37.4'' Lupinus polyphyllus Lindl. Jihlava Jihlava meadow, 2.2 km NNE of the railway station Jihlava 49 26'00.5'' 15 35'59.3'' Lychnis coronaria (L.) Desr. Strakonice Strakonice road edge at the summerhouse area, 1.5 km NNW of the castle Strakonice 49 16'17.4'' 13 53'40.9'' Lychnis coronaria (L.) Desr. Všenory Praha-západ road edge at U potoka street in the village 49 55'49.9'' 14 18'03.9'' Lychnis coronaria (L.) Desr. Bojanovice Praha-západ road edge, 0.6 km NNW of the church Bojanovice 49 51'32.8'' 14 20'55.0'' Lysimachia punctata. L. Černolice Praha-západ road edge, 1.7 km SSE of the railway station Dobřichovice 49 54'47.7'' 14 17'58.7'' Lysimachia punctata. L. Smolné pece Karlovy Vary road edge at the N edge of the village 50 17'35.0" 12 45'58.8" 5

Lysimachia punctata. L. Čelina Příbram Čelinský potok brook bank, 0.5 km NW of the Čelina castle 49 44'14.7" 14 18'57.5" Matricaria discoidea DC. Dobřichovice Praha-západ ruderal site, 0.2 km NNE of the railway station Dobřichovice 49 55'45.1" 14 17'27.3" Matricaria discoidea DC. Řepešín-Záblatí Prachatice pasture at the W edge of the Řepešín village 49 00'08.2" 13 55'34.2 Matricaria discoidea DC. Praha-Modřany Praha road edge, Vokrojova street in the town 50 00'05.4'' 14 25'42.5'' Matricaria discoidea DC. Kopřivnice Nový Jičín road edge, 0.2 km SSE of the railway station Kopřivnice 49 35'36.0'' 18 08'50.8'' Medicago sativa L. Praha-Modřany Praha road edge, Generála Šišky street, 1 km E of the railway station Praha-Modřany 50 00'12.7'' 14 24'59.2'' Medicago sativa L. Praha-Barandov Praha road bank at K Barandovu street, SSW of the railway station Praha- Hlubočepy 50 01'46.6'' 14 22'48.5'' Mimulus guttatus DC. Všenory Praha-západ Všenorský potok brook bank, 0.4 km WSW of the railway station Všenory 49 55'54.6'' 14 17'57.6'' Mimulus guttatus DC. Kvilda-Františkov Prachatice wet meadow, near Teplá Vltava river, 3.8 km SE of the Kvilda church 49 00'00.9'' 13 37'22.1'' Mimulus guttatus DC. Horažďovice Klatovy wet meadow, near Otava river, 3.8 km SE of the Horažďovice church 49 18'30.6" 13 44'4.79" Myrrhis odorata (L.) Scop. Velká Úpa Trutnov road bank, 0.5 km NE of the Velká Úpa church 50 41'34.2'' 15 46'46.0'' Myrrhis odorata (L.) Scop. Jizerka-Kořenov Jablonec nad Nisou meadow near the chalet, 4.8 km NNE of the railway station Kořenov 50 48'41.5'' 15 21'07.9'' Myrrhis odorata (L.) Scop. Josefův Důl Jablonec nad Nisou meadow, 0.6 km SSW of the railway station Josefův Důl 50 46'21.6'' 15 14'15.2'' Oenothera biennis L. Praha-Modřany Praha road edge, U kina street 50 00'13.1'' 14 24'13.3'' Oenothera biennis L. Velký Osek Kolín road edge, 4 km NW of the railway station Velký Osek 50 07'02.5'' 15 08'27.8'' Oenothera glazioviana M. Micheli Černolice Praha-západ road edge at the summerhouse area, 1.7 km SSE of the railway station Dobřichovice 49 54'47.7'' 14 17'58.7'' Oxalis corniculata var. repens (Thunb.) Zucc. Dobřichovice Praha-západ road edge at the summerhouse area, 0.2 km NNE of the railway station Dobřichovice 49 55'43.8'' 14 17'23.5'' Oxalis corniculata var. repens (Thunb.) Zucc. Černolice Praha-západ road edge at the summerhouse area, 1.7 km SSE of the railway station Dobřichovice 49 54'46.5" 14 17'57.6" Oxalis corniculata var. repens (Thunb.) Zucc. Velký Osek Kolín road edge in the village, 0.6 km SSW of the railway station Velký Osek 50 05'54.9'' 15 11'19.1'' Oxalis corniculata var. repens (Thunb.) Zucc. Praha-Podolí Praha road edge at the Pod Pekařkou street in the town 50 02'38.7" 14 25'09.2" Oxalis dillenii Jacq. Lanžhot Břeclav road edge near Polínský vrch hill, 11.1 km SSW of the railway station Lanžhot 48 38'48.5'' 16 57'34.7'' Oxalis dillenii Jacq. Velký Osek Kolín road edge in the village, 0.6 km SSW of the railway station Velký Osek 50 05'49.3'' 15 11'26.9'' Oxalis dillenii Jacq. České Budějovice České Budějovice lawn in the campus of Secondary school 48 58'44.5" 14 29'52.3'' Oxalis dillenii Jacq. Lanžhot Břeclav sandpit bank, 11.2 km S of the railway station Lanžhot 48 37'58.6'' 16 57'38.9'' Oxalis fontana Bunge Černolice Praha-západ road edge at the summerhouse area, 1.7 km SSE of the railway station Dobřichovice 49 54'47.4" 14 17'59.1" Oxalis fontana Bunge Praha-Modřany Praha road edge at Dolnocholupická street in the town 49 59'58.3" 14 24'38.8" Oxalis fontana Bunge Dubňany Hodonín forest near former Důl 1. máj mine 48 54'20.7" 17 04'27.2" Oxybaphus nyctagineus (Michx.) Sweet Břeclav Břeclav railway bank at the railway station Břeclav-zastávka 48 45'47.4'' 16 54'10.4'' Panicum capillare L. Týniště nad Orlicí Rychnov nad Kněžnou railway station Týniště nad Orlicí 50 09'39.7'' 16 04'07.0'' Panicum capillare L. Velký Osek Kolín Labe river bank, 2.7 km WNW of the railway station Velký Osek 50 06'27.4'' 15 09'30.3'' Panicum capillare L. Libice nad Cidlinou Nymburk road edge, 0.3 km ENE of railway station Libice nad Cidlinou 50 07'41.7'' 15 11'16.0'' Panicum miliaceum L. Sedlec Břeclav field edge, 0.8 km SE of railway station Sedlec 48 46'17.8'' 16 42'39.8'' 6

Panicum miliaceum L. Mikulov-nádraží Břeclav railway station Mikulov 48 48'01.2'' 16 37'37.6'' Panicum miliaceum L. Domanice Strakonice field edge, 0.5 km SSE of the railway station Domanice 49 17'45.1'' 13 55'00.0'' Phytolacca esculenta Van Houtte Černošice Praha-západ alluvium of the Berounka river, 1.1 km NNE of the railway station Černošice 49 58'00.8'' 14 20'11.8'' Phytolacca esculenta Van Houtte Břeclav Břeclav railway embankment near the railway station Břeclav-zastávka 48 46'28.1'' 16 54'48.9'' Phytolacca esculenta Van Houtte Dobřichovice Praha-západ road edge, 0.6 km NW of the railway station Dobřichovice 49 55'45.5'' 14 16'46.8'' Potentila intermedia L. Lanžhot Břeclav railway station Lanžhot 48 43'58.7'' 16 57'41.2'' Potentila intermedia L. Týniště nad Orlicí Rychnov nad Kněžnou railway station Týniště nad Orlicí 50 09'40.5'' 16 04'03.4'' Rudbeckia hirta L. Černolice Praha-západ road edge at the summerhouse area, 1.7 km SSE of the railway station Dobřichovice 49 54'47.7'' 14 17'58.7'' Rudbeckia laciniata L. Kolín Kolín Labe river bank, 0.7 km NNW of the railway station Kolín-zastávka 50 02'09.6'' 15 11'23.5'' Rudbeckia laciniata L. Lanžhot Břeclav wet meadow, 2.7 km SSE of the railway station Lanžhot 48 23'34.0'' 16 58'24.9'' Rudbeckia laciniata L. Kostice Břeclav forest clearing, 3.7 km of the railway station Lanžhot 48 44'58.6'' 17 00'17.8'' Rudbeckia laciniata L. Nasavrky Chrudim Chrudimka river bank at the summerhouse area, 1.7 km NNE of the Nasavrky church 49 51'37.9' 15 48'20.9'' Rumex alpinus L. Pec pod Sněžkou Trutnov road edge, 1.4 km NNW of the Pec pod Sněžkou church 50 41'28.6'' 15 42'54.1'' Rumex alpinus L. Malá Úpa Trutnov road edge, 0.6 km WSW of the municipality Malá Úpa village 50 44'09.9'' 15 47'54.3'' Rumex alpinus L. Jizerka, Kořenov Jablonec nad Nisou road edge, 5.7 km NNW of the railway station Kořenov 50 49'17.8" 15 20'46.8" Rumex longifolius DC. Pec pod Sněžkou Trutnov road edge, 1.4 km NNW of the Pec pod Sněžkou church 50 41'28.6'' 15 42'54.1'' Rumex longifolius DC. Malá Úpa Trutnov road edge, 0.6 km WSW of the municipality Malá Úpa village 50 44'09.9'' 15 47'54.3'' Rumex patientia L. subsp. patientia Milovice Břeclav road edge, 0.3 km W of the municipality of Milovice village 48 51'08.5'' 16 41'43.9'' Rumex patientia L. subsp. patientia Praha-Vysočany Praha railway edge, 0.9 km ENE of the railway station Praha-Vysočany 50 06'47.0'' 14 30'42.0'' Rumex patientia L. subsp. patientia Pavlov Břeclav road bank, 1 km ESE of the Pavlov church 48 52'14.3'' 16 41'02.5'' Rumex thyrsiflorus Fingerh. Velký Osek Kolín meadow, 2.7 km WNW of the railway station Velký Osek 50 06'30.6'' 15 09'26.9'' Rumex thyrsiflorus Fingerh. Všenory Praha-západ meadow, 0.3 km SSW of the railway station Všenory 49 55'56.1'' 14 18'02.8'' Rumex thyrsiflorus Fingerh. Lanžhot Břeclav road edge, 3 km WNW of the railway station Lanžhot 48 44'32.1'' 16 56'13.4'' Rumex thyrsiflorus Fingerh. Hrušky Břeclav sandpit bank, 4.2 km WSW of the railway station Hrušky 48 46'33.3" 16 55'36.4" Rumex thyrsiflorus Fingerh. Libice nad Cidlinou Nymburk road edge, 0.3 km ENE of railway station Libice nad Cidlinou 50 07'43.2" 15 11'11.6" Scutellaria altissima L. Roztoky Praha-západ forest near the Unětický potok brook, 1.2 km SSW of the railway station Roztoky 50 09'02.2'' 14 23'13.9'' Scutellaria altissima L. Měšice u Prahy Praha-východ forest at the N edge of the Park Měšice 50 11'47.6'' 14 31'12.8'' Scutellaria altissima L. Srbsko Beroun forest edge, 2 km ESE of the railway station Srbsko 49 55'55.5'' 14 09'23.2'' Sedum hispanicum L. Velký Osek Kolín road edge, 3.8 km NW of railway station Velký Osek 50 07'03.4'' 15 08'55.8'' Sedum hispanicum L. Mníšek pod Brdy Praha-západ road edge, 3.4 km NNE of the railway station Mníšek pod Brdy 49 53'00.6'' 14 16'45.8'' Sedum rupestre L. subsp. erectum t Hart Štramberk Nový Jičín limestone quarry Kamenárka, 0.8 km NW of railway station Štramberk 49 35'25.7'' 18 07'21.7'' Sedum rupestre L. subsp. erectum t Hart Praha-Zbraslav Praha rock in the forest, 0.4 km SSE of the railway station Praha-Zbraslav 49 58'00.0'' 14 24'06.5'' Sedum spurium M. Bieb. Kobylí Břeclav railway station Kobylí 48 56'02.7'' 16 52'52.2'' Senecio inaequidens DC. Roztoky Praha-západ road edge, 0.5 km S of the railway station Roztoky 50 09'15.3'' 14 23'53.7'' Senecio inaequidens DC. Sedlec-Praha Praha railway bank, 1 km S of the railway station Praha-Sedlec 50 07'21.7" 14 23'38.0" 7

Senecio vernalis W. et K. Tuhaň Mělník Červená píska sandpit, 1.8 km ESE of the Tuhaň church 50 17'37.1'' 14 32'31.1'' Senecio vernalis W. et K. Veltrusy Mělník field, 1.8 km SE of the Veltrusy church 50 15'37.8'' 14 20'27.0'' Senecio vernalis W. et K. Píšťany Litoměřice Žernosecké jezero sandpit, 0.8 km SW of the railway station Velké Žernoseky 50 31'12.2'' 14 03'55.4'' Setaria faberi F. Herrmann Kolín-Záblatí Kolín railway station Kolín-Záblatí 50 02'10.6'' 15 12'37.6'' Silene dichotoma Ehrh. Hustopeče Břeclav road bank, 3.2 km SE of the railway station Hustopeče 48 55'09.0'' 16 46'12.0'' Silene dichotoma Ehrh. Milovice Břeclav vineyard bank, 0,4 km WSW of the church Milovice 48 51'06.6'' 16 41'32.1'' Sisymbrium altissimum L. Tuhaň Mělník Červená píska sandpit, 1.8 km ESE of the Tuhaň church 50 17'37.9'' 14 32'30.4'' Sisymbrium altissimum L. Mutěnice Hodonín railway bank, 0.5 km E of the railway station Mutěnice 48 54'16.8'' 17 02'52.7'' Sisymbrium altissimum L. Pečky Kolín ailway station Pečky 50 05'28.9'' 15 01'15.8'' Sisymbrium altissimum L. Hrušky Břeclav sandpit bank, 4.2 km WSW of the railway station Hrušky 48 46'33.3" 16 55'36.4" Sisymbrium altissimum L. Velký Osek Kolín road edge, 3.8 km NW of railway station Velký Osek 50 07'03.7'' 15 08'56.7'' Sisymbrium loeselii L. Praha-Braník Praha railway station Praha-Braník 50 02'08.3" 14 24'54.3" Sisymbrium loeselii L. Lanžhot Břeclav road edge, 1.3 km WSW of the railway station Lanžhot 48 43'32.8'' 16 56'57.9'' Sisymbrium loeselii L. Pečky Kolín railway station Pečky 50 05'29.5'' 15 01'13.0'' Sisymbrium loeselii L. Praha-Komořany Praha railway, 0.3 km S of the railway station Praha-Komořany 50 00'09.3'' 14 24'11.3'' Sisymbrium strictissimum L. Vacíkov Příbram road bank, 3.1 km SW of the Hvožďany church 49 32'41.7'' 13 50'09.2'' Sisymbrium strictissimum L. Strakonice Strakonice railway bank, 0.2 km S of the Strakonice castle 49 15'21.3" 13 54'04.3" Sisymbrium strictissimum L. Praha-Komořany Praha road edge, 0.3 km S of the railway station Praha-Komořany 49 59'39.8" 14 24'20.1" Sisymbrium strictissimum L. Lánov Trutnov road edge, 3 km NE of the railway station Lánov 50 37'56.3'' 15 39'51.2'' Smyrnium perfoliatum L. Praha-Zbraslav Praha forest edge, 0.7km SE of the railway station Praha-Zbraslav 49 58'03.0'' 14 24'27.8'' Smyrnium perfoliatum L. Praha-Petřín Praha forest edge, 0.2km WSW of the lower funicular station 50 04'57.2'' 14 24'03.1'' Smyrnium perfoliatum L. Praha-Liboc Praha forest edge, 1km SSE of the Svatý Fabián Church 50 05'45.3'' 14 19'20.7'' Solidago canadensis L. Nový Bydžov Hradec Králové ruderal site, 2.5 km E of railway station Nový Bydžov 50 14'50.1'' 15 30'52.6'' Solidago canadensis L. Praha-Komořany Praha road edge, 0.3 km NNE of the railway station Komořany 49 58'02.3'' 14 24'30.4'' Solidago canadensis L. Dobřichovice Praha-západ ruderal site, 0.2 km NE of the railway station Dobřichovice 49 55'41.6'' 14 17'23.1'' Solidago canadensis L. Všenory Praha-západ ruderal site, 0.3 km WSW of the railway station Všenory 49 55'54.4'' 14 18'08.5'' Solidago canadensis L. Vilsnice Děčín ruderal site, 0.9 km S of railway station Vilsnice 50 44'15.1'' 14 10'53.0'' Solidago canadensis L. Beroun Beroun railway station Beroun 49 57'24.4'' 14 04'26.1'' Solidago gigantea Ait. Stráž nad Ohří Karlovy Vary Ohře river bank, 0.5 km SW of the railway station Stráž nad Ohří 50 20'13.1'' 13 03'11.2'' Solidago gigantea Ait. Mutěnice Hodonín railway bank, 0.6 km ENE of the railway station Mutěnice 48 54'17.8'' 17 02'59.6'' Solidago gigantea Ait. Čelákovice Praha-východ Labe river bank, 0.4 km NNE of railway station Čelákovice-Jiřina 50 10'19.4'' 15 46'09.5'' Solidago gigantea Ait. Liteň Beroun railway station Liteň 49 54'12.6'' 14 09'15.9'' Sorghum halepense (L.) Pers. Kolín-Záblatí Kolín railway station Kolín-Záblatí 50 02'10.6'' 15 12'37.6'' Telekia speciosa (Schreber) Baumg. Čelina Příbram Čelinský potok brook bank, 0.5 km NW of the Čelina castle 49 43'59.7'' 14 19'56.6'' Telekia speciosa (Schreber) Baumg. Průhonice Praha-západ forest edge in the Park Průhonice 49 59'49.3'' 14 33'50.3'' Trifolium hybridum L. Vilsnice Děčín ruderal site, 0.9 km S of railway station Vilsnice 50 44'15.1'' 14 10'53.0'' Trifolium hybridum L. Praha-Komořany Praha Vltava river bank, 0.3 km NW of the railway station Praha-Komořany 49 59'37.3'' 14 24'04.8'' 8

Veronica persica Poiret Bolehošť Rychnov nad Kněžnou ruderal site in the village 50 22'50.2'' 16 06'05.0'' Veronica persica Poiret Domkov Náchod field edge, 3.9 km NW of the railway station Nové Město nad Metují 50 22'50.3'' 16 06'01.4'' Veronica persica Poiret Průhonice Praha-západ ruderal site in the Park Průhonice 49 59'42.3" 14 33'59.6" Vicia grandiflora Scop. Slaník Strakonice road edge, 2.8 NE of the railway station Strakonice 49 16'10.9'' 13 56'57.4'' Virga strigosa (R. et Sch.) Holub Libeznice Praha-východ road edge, 1.1 WNW of the railway station Měšice u Prahy 50 12'19.9'' 14 29'54.9'' Virga strigosa (R. et Sch.) Holub Praha-Cholupice Praha road edge at the NW edge of Cholupická bažantnice pheasantry natural monument 49 59'05.8'' 14 27'17.8'' Virga strigosa (R. et Sch.) Holub Srbsko Beroun road edge, 2 km ESE of the railway station Srbsko 49 55'55.1'' 14 09'20.9'' Xanthium albinum (Widd.) H. Scholtz et Sukopp Lanžhot Břeclav road edge, 12.3 km SSW of the railway station Lanžhot 48 37'28.6'' 16 55'59.6'' Xanthium albinum (Widd.) H. Scholtz et Sukopp Hrušky Břeclav sandpit bank, 4.2 km WSW of the railway station Hrušky 48 46'37.9'' 16 55'31.9'' Xanthium albinum (Widd.) H. Scholtz et Sukopp Velké Březno Ústí nad Labem Labe river bank, 0.7 km NW of the west edge of the village 50 40'04.3" 50 40'04.3" Xanthium albinum (Widd.) H. Scholtz et Sukopp Píšťany Litoměřice Žernosecké jezero sandpit bank, 0.8 km SW of the railway station Velké Žernoseky 50 31'12.5'' 14 03'59.5'' 9