List of wild Hieracium subgen. Pilosella plants used for genome size estimation. Detailed information is given about original locality (including

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1 Supplementary Information List of wild Hieracium subgen. Pilosella plants used for genome size estimation. Detailed information is given about original locality (including geographic co-ordinates and altitude), collector(s), year of collection, and name(s) of hawkweed specialists revising plant samples together with somatic chromosome numbers and breeding systems for 278 accessions (184 basic species, 87 intermediate = hybridogenous species, and seven recent natural crosses) Accession Locality*, collector(s), year of collection, taxonomic revision Latitude Longitude Altitude Chromosome Breeding number N E number (2n) system Basic species H. aurantiacum L., 2n = 4x 119 AU CZ, Krkonoše Mts, Horní Mísečky, FK & AK 1997, rev. SB 50 44'10'' 15 34'10'' 1060 m 36 a apomictic 134/1 AU CZ, Krkonoše Mts, Velká Úpa - village, FK 1998, rev. SB 50 41'30'' 15 45'00'' 760 m 36 b NS 136 AU CZ, Krkonoše Mts, Hlušiny, FK 1998, rev. SB 50 40'50'' 15 46'30'' 930 m 36 a NS 192 AU CZ, Krkonoše Mts, Pec pod Sněžkou, FK 1999, rev. SB 50 41'50'' 15 43'50'' 830 m 36 b NS 20 AU CZ, Krkonoše Mts, Velká Úpa, Prostřední Výsluní, FK & AK 1997, rev. SB 50 41'40'' 15 47'10'' 910 m 36 a apomictic a 223 AU CZ, Krkonoše Mts, Dolní Malá Úpa, Moravanka, FK, AK, SB & JC '50'' 15 49'10'' 1050 m 36 b NS 252 AU CZ, Šumava Mts, former settlement of Gerlova Huť near Hartmanice, FP 1999, rev. SB & FS 49 09'54'' 13 16'44'' 950 m 36 c NS 270 AU CZ, Šumava Mts, Kvilda, FK '30'' 13 34'10'' 1080 m 36 c NS 30 AU CZ, Krkonoše Mts, Velká Úpa - Janovy Boudy, FK 1995, rev. SB 50 41'20'' 15 48'00'' 860 m 36 a apomictic a 387 AU CZ, Krušné hory Mts, Přebuz, FK '00'' 12 30'00'' 890 m 36 d NS 94 AU CZ, Krkonoše Mts, Horní Malá Úpa Pomezní Boudy, FK 1997, rev. SB 50 44'10'' 15 48'10'' 950 m 36 a NS 451 AU CZ, Krkonoše Mts, in the valley below Dolní Malá Úpa FK, AK & JC 2000, rev. SB 50 42'50'' 15 48'50'' 880 m 36 NS 546a AU CZ, Šumava Mts, Hadí vrch near Hartmanice, FP & FK 2001, rev. SB & FS 49 11'05'' 13 20'45'' 960 m 36 NS 895 AU CZ, Šumava Mts, Slučí Tah near Hartmanice, FK 2003, rev. SB & FS 49 09'10'' 13 21'00'' 880 m NC apomictic 905 AU CZ, Šumava Mts, Zhůří near Železná Ruda, FK 2003, rev. SB & FS 49 10'45'' 13 20'10'' 930 m NC NS 576a AU CZ, Ještědský hřeben Mts, Kryštofovo Údolí near Chrastava, FK & SB '04'' 14 55'57'' 440 m NC NS 842 AU D, North Rhine Westfalia, Hagen - Eilpe, Krähnocken, above Eilper Hangstieg, UR ' 7 28' 260 m NC NS 838 AU D, North Rhine Westfalia, Hagen - Eilpe, Krähnocken, above Eilper Hangstieg, UR ' 7 28' 260 m NC NS 1

2 H. aurantiacum L., 2n = 5x 135 AU CZ, Krkonoše Mts, Velká Úpa, FK 1998, rev. SB 50 41'20'' 15 45'30'' 720 m 45 b NS 265 AU CZ, Krkonoše Mts, Zadní Rennerovky, FK 1999, rev. SB 50 42'00'' 15 40'00'' 1240 m 45 b apomictic b 915 AU CZ, Krkonoše Mts, Rýchory, FK 2003, rev. SB 50 39'10'' 15 52'10'' 960 m 45 NS 769/1 AU CZ, Šumava Mts, Zhůří near Kašperské Hory, FP, FK, SB & FS '10'' 13 33'20'' 1120 m NC apomictic 804 AU A, Allgäuer Alpen Mts, Wildental, HWS '40'' 10 11'10'' 1900 m NC NS 824 AU/3 RO, Tarcului Mtii Mts, Tomeasa Mt, PM '15'' 22 42'10'' 1800 m NC NS H. bauhinii Besser, 2n = 4x 491 BA CZ, Praha Vysočany, near the railway station, FK '45'' 14 29'50'' 210 m 36 apomictic 591/1 BA CZ, Olomouc - Holice, FK '40'' 17 17'00'' 210 m NC NS H. bauhinii Besser, 2n = 5x 589 BA SK, Velká Fatra Mts, Tlstá Mt, FK '00'' 18 58'30'' 1370 m NC NS 607 BA CZ, Lovosice, Malé Žernoseky, FK '25'' 14 03'15'' 200 m NC apomictic 615 BA CZ, Roudnice, Drahobuz, FK '30'' 14 20'20'' 220 m NC NS 655 BA CZ, Praha Vysočany, near the railway station, FK & AK '45'' 14 29'50'' 210 m 45 apomictic 659 BA CZ, Praha Vysočany, near the railway station, FK & AK 2002 (a clone different from 655) 50 06'45'' 14 29'50'' 210 m 45 NS 676 BA CZ, Dolní Dunajovice near Mikulov, FK, OR & JD '20'' 16 33'05'' 240 m NC apomictic 687 BA CZ, Sedlec near Mikulov, FK, OR & JD '45'' 16 40'20'' 190 m NC NS 718 BA CZ, Lomnice nad Popelkou, Ploužnice, FK '40'' 15 23'10'' 520 m NC NS 720 BA CZ, Rovensko pod Troskami - Semínova Lhota, FK '05'' 15 16'30'' 340 m NC apomictic H. bauhinii Besser, 2n = 6x 126 BA CZ, Valov near Podbořany, FK '50'' 13 25'00'' 350 m 54 c apomictic c 463 BA CZ, Rozkoš near Česká Skalice, FK '00'' 16 02'45'' 300 m 54 NS 700 BA CZ, Brno, Kamenný kopec hill, FK & OR '50'' 16 33'15'' 370 m NC apomictic H. bauhinii Besser, 2n = 7x 492 BA CZ, Praha Vysočany, near the railway station, FK '45'' 14 29'50'' 210 m 63 apomictic H. caespitosum Dumort., 2n = 4x 113 CE CZ, Krkonoše Mts, Pec pod Sněžkou, FK & AK 1997, rev. SB 50 41'20'' 15 43'10'' 910 m 36 a apomictic b 358 CE PL, Góry Izerskie Mts, Velká Jizerská louka meadow close to Czech-Polish border, FK 2000, 50 51'00'' 15 21'40'' 830 m 36 d NS rev. HWS 364 CE D, East Lusatian Hills, Piessnitz valley near Schönau-Berzdorf, FK & AK 2000, rev. HWS 51 03'35'' 14 54'04'' 220 m 36 d NS 369 CE D, East Lusatian Hills, eastern edge of Schönau-Berzdorf, FK & AK 2000, rev. HWS 51 03'50'' 14 53'50'' 200 m 36 d NS 452 CE CZ, Krkonoše Mts, Velká Úpa, FK, AK & JC 2000, rev. SB 50 41'53'' 15 46'05'' 900 m 36 NS 748 CE CZ, Šumava Mts, Hadí vrch near Hartmanice, FP & FK 2001, rev. SB & FS 49 11'05'' 13 20'45'' 960 m NC NS 757 CE CZ, Šumava Mts, Slučí Tah near Hartmanice, FP & FK 2001, rev. SB & FS 49 09'10'' ' 880 m NC NS 881 CE CZ, Šumava Mts, Zhůří near Kašperské Hory, FK 2003, rev. SB & FS 49 05'10'' 13 33'20'' 1120 m NC NS 2

3 H. caespitosum Dumort., 2n = 5x caec. Hir 3 D, Erzgebirge Mts, Hirtstein, SB '02'' 13 10'24'' 880 m NC NS H. cymosum L. subsp. cymosum, 2n = 2x 10/3 CY CZ, Křivoklátsko, Nižbor, at the rock Čerchov, RŠ '18'' 13 58'44'' 240 m 18 (counted by RŠ) sexual 11/12 CY CZ, Křivoklátsko, between Račice and Zbečno, RŠ '34'' 13 54'30'' 250 m 18 (counted by RŠ) sexual 13/7 CY CZ, České středohoří Mts, Bělušice, Milá hill, RŠ '02'' 13 45'24'' 510 m 18 (counted by RŠ) sexual 14/11 CY CZ, České středohoří Mts, Lovosice, Boreč hill, RŠ '55'' 13 59'12'' 420 m 18 (counted by RŠ) sexual 15/9 CY CZ, Bohemian Karst, between Karlštejn and Zadní Třebáň, RŠ '01'' 14 11'45'' 300 m 18 (counted by RŠ) sexual 16/4 CY CZ, Praha-Bohnice, RŠ '30'' 14 23'58'' 190 m 18 (counted by RŠ) sexual 2/5 CY CZ, Zrůbek, at the Slapská přehrada reservoir, RŠ '43'' 14 17'30'' 270 m 18 (counted by RŠ) sexual 20/3 CY CZ, Moravský Krumlov, Sv. Florián hill, RŠ '51'' 16 19'10'' 280 m 18 (counted by RŠ) sexual 3/2 CY CZ, Cholín, at the Slapská přehrada reservoir, RŠ '22'' 14 19'36'' 350 m 18 (counted by RŠ) sexual 4/3 CY CZ, Labské středohoří Mts, Sebuzín, Havraní skála, RŠ '34'' 14 04'26'' 390 m 18 (counted by RŠ) sexual 42/3 CY SK, Slanské vrchy hills, Slanec, Slanský hradný vrch hill, RŠ '11'' 21 28'22'' 520 m 18 (counted by RŠ) sexual 43/1 CY SK, Slovenské rudohorie Mts, Jelšavská Teplica, Muteň hill, RŠ '00'' 20 18'00'' 400 m 18 (counted by RŠ) sexual 44/2 CY SK, Štiavnické vrchy, Hliník nad Hronom, nature reserve Kapitulské bralá, RŠ '33'' 18 51'00'' 320 m 18 (counted by RŠ) sexual 48/5 CY CZ, Tasovice, Tasovické svahy above Dyje river, RŠ & JC '51'' 16 08'21'' 200 m 18 (counted by RŠ) sexual 6/9 CY CZ, Výří skály near Tukleky, near the Orlík reservoir, RŠ '26'' 14 11'10'' 420 m 18 (counted by RŠ) sexual 7/10 CY CZ, Oslov near the Orlík reservoir, Krkavčina pine-wood, RŠ '00'' 14 11'06'' 420 m 18 (counted by RŠ) sexual 9/5 CY CZ, Bohemian Karst, Srbsko, Velká hora hill, RŠ '56'' 14 09'25'' 350 m 18 (counted by RŠ) sexual H. cymosum L. subsp. cymosum, 2n = 4x 21/6 CY CZ, Adamov 1, slopes above the Svitava river, RŠ '45'' 16 40'07'' 300 m NC NS 22/7 CY CZ, Adamov 2, slopes above the Svitava river, RŠ '47'' 16 40'08'' 320 m NC NS 47/5 CY CZ, Lukov, Vraní skála rock above the Dyje river, RŠ & JC '59'' 15 53'45'' 360 m 36 NS H. cymosum L. subsp. cymigerum Reichenb., 2n = 5x 722 CY CZ, Krkonoše Mts, Víchovská Lhota, FK 2002, rev. SB 50 38'55'' 15 29'15'' 580 m 45 NS H. echioides L., 2n = 2x 30/2 A, Niederösterreich, 1 km N of the village of Markhof, TP, JC & DH m 18 c sexual 31/1 A, Niederösterreich, Mt. Braunsberg near Hainburg an der Donau, TP, JC & DH m 18 c sexual 37/c CZ, P: the valley of the Dyje river SE of the village of Dyje, TP m 18 c sexual 39/a CZ, Cínová hora hill near Znojmo, TP m 18 c sexual 48/e CZ, hill at the NE margin of Hostěradice, TP m 18 c sexual 51/a D, Brandenburg, distr.: Barnim: Pimpinellenberg hill near Oderberg, TP & JC m 18 c sexual 56/3 PL, Skorocice, the nature reserve near the village, TP, JC & ZS m NC sexual 58/1 PL, Przemyśl, along the road from the town to the Ukrainian border, TP, JC & ZS m NC sexual 58/23 PL, Przemyśl, along the road from the town to the Ukrainian border, TP, JC & ZS m NC sexual 3

4 59/2 SK, Tarbucka hill in Zemplínske vrchy Mts, TP & JC m NC sexual 83/1 H, Fenyőfő in Bakony Mts, TP & JC m 18 (counted by TP) sexual 84/3 H, Fenyőfő in Bakony Mts, TP & JC m NC sexual 85/2 H, Fenyőfő in Bakony Mts, TP & JC m NC sexual 93/1 CZ, České středohoří Mts, Litoměřice, Radobýl hill, TP & HWS m NC sexual HAV S 54 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC m 18 (counted by TP) sexual HV 4 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC m NC sexual H. echioides L., 2n = 3x 22/d CZ, between Konice and Popice near Znojmo, TP N m 27 c sexual HAV J 22 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC m 27 (counted by TP) sexual HAV J 33 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC m 27 (counted by TP) sexual HAV S 59 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC m NC sexual HV 10 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC m 27 (counted by TP) sexual HV 24 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC m NC sexual HV 27 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC m 27 (counted by TP) sexual HV 41 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC m 27 (counted by TP) sexual HV 5 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC m NC sexual HV 6 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC m 27 (counted by TP) sexual H. echioides L., 2n = 4x 30/3 A, Niederösterreich, 1 km N of the village of Markhof, TP, JC & DH m 36 c sexual 4/3 CZ, Šobes vineyards near Hnanice, TP m 36 c sexual HAV J 1 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC m 36 (counted by TP) sexual HV 11 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC m NC sexual HV 12 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC m NC sexual HV 13 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC m NC sexual HV 14 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC m NC sexual HV 15 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC m NC sexual HV 20 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC m NC sexual HV 21 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC m 36 (counted by TP) sexual HV 29 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC m 36 (counted by TP) sexual HV 34 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC m NC sexual H. echioides L., 2n = 5x 38/a CZ, Havranické vřesoviště heathland near Havraníky, TP & JC m 45 d sexual 7/2 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC m 45 (counted by TP) sexual HV 28 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC m 45 d sexual HV 30 CZ, Havranické vřesoviště heathland near Havraníky, TP & JC m NC sexual 4

5 H. hoppeanum Schultes, 2n = 2x 781 HO D, Ausburg, Nature reserve Ausburger Wald, near Mering, MC '25'' 10 54'15'' 510 m NC NS H. hoppeanum subsp. testimoniale Nägeli ex Peter (syn. H. macranthum (Ten.) Ten.), 2n = 2x 948 MC BG, Vračanska Planina Mts, between Vratsa and Parševitsa hut, FK, AK & VV 2005, rev. SB 43 10'35'' 23 28'55'' 1050 m NC NS 962 MC BG, Vračanska Planina Mts, between Vratsa and Parševitsa hut, FK, AK & VV 2005, rev. SB 43 10'15'' 23 28'50'' 1160 m NC NS 969 MC BG, near the dam of Černila, S of the town of Montana, FK, AK & VV 2005, rev. SB 43 22'30'' 23 14'40'' 190 m NC NS 1008 MC BG, Eastern Rodopi Mts, E of the town of Kardžali, FK, AK & VV 2005, rev. SB 41 38'10'' 25 25'50'' 260 m 18 (counted by VV) sexual H. lactucella Wallr., 2n = 2x 259 LA SK, Velká Fatra Mts, Malá Krížna Mt, FK & AK 1999, rev. HWS 48 52'40'' 19 03'40'' 1500 m NC NS 260 LA SK, Západné Tatry Mts, Osobitá Mt, FK & AK 1999, rev. HWS 49 15'40'' 19 43'20'' 1570 m 18 c NS 269 LA CZ, Šumava Mts, Kvilda, FK 1999, rev. SB & FS 49 00'40'' 13 34'40'' 1050 m 18 c NS 272 LA CZ, Šumava Mts, Gerlova Huť near Železná Ruda, FK 1999, rev. SB & FS 49 10'00'' 13 20'00'' 920 m 18 c NS 277 LA CZ, Krkonoše Mts, Velká Úpa - Janovy Boudy, FK 1999, rev. SB 50 41'20'' 15 48'00'' 860 m 18 NS 44 LA CZ, Krkonoše Mts, Velká Úpa - Janovy Boudy, FK 1995, rev. SB 50 41'20'' 15 48'00'' 860 m 18 a sexual a 436 LA CZ, Krkonoše Mts, Horní Malá Úpa Pomezní Boudy, FK, AK & JC 2000, rev. SB 50 44'20'' 15 48'50'' 1000 m 18 NS 529 LA CZ, Brdy Hills, Dolní Kvaň near Hořovice, JH '10'' 13 51'00'' 520 m 18 d NS 786 LA CZ, Českomoravská vrchovina Highlands, Cikháj, OR '07'' 15 57'54'' 650 m 18 c NS 787 LA SK, Velká Fatra Mts, Baník hill near Donovaly, OR '50'' 19 13'30'' 1060 m 18 d NS 789 LA D, Oberpfalzer Wald, Wümberg-Köblitz, FK '00'' 12 11'40'' 460 m NC NS 790 LA A, Allgäuer Alpen Mts, Massife of Widderstein Mt, FK & AK 2002, rev. HWS 47 16'15'' 10 07'00'' 1900 m NC NS 806 LA A, Allgäuer Alpen Mts, W of Walmendiger Horn Mt near Baad, FK & AK 2002, rev. HWS 47 19'55'' 10 07'15'' 1450 m NC NS 865 LA CZ, Šumava Mts, Jelení Vrchy, FK 2003, rev. SB & FS 48 57'00'' 13 47'10'' 820 m NC NS 889 LA CZ, Šumava Mts, Filipova Huť, FK 2003, rev. SB & FS 49 01'25'' 13 30'40'' 1060 m NC NS lac.hot 1 D, Erzgebirge Mts, Schellerhau - Hotel, SB '38'' 13 41'24'' 770 m NC NS lac. Jon 2 D, Oberlausitz, Jonsdorf, SB '34'' 14 42'30'' 510 m NC NS lac. Neu 1 D, Erzgebirge Mts, Neuwernsdorf, SB '07'' 13 31'17'' 600 m NC NS lac. ZwW 1 D, Erzgebirge Mts, Zweibach West, SB '04'' 12 52'20'' 770 m NC NS H. onegense (Norrl.) Norrl., 2n = 2x 261 CE/2 SK, Západné Tatry Mts, near Zverovka chalet, FK & AK 1999, rev. HWS 49 17'00'' 19 42'00'' 1080 m 18 c NS 6/4 SP2 CZ, Krkonoše Mts, Velká Úpa - Janovy Boudy, FK & AK 1998, rev. SB 50 41'20'' 15 47'50'' 870 m 18 b sexual b RS 17/6 CZ, Hodonín, the nature reserve Dúbrava, RS '18'' 17 06'06'' 170 m 18 (counted by RŠ) NS 946 ON BG, Vračanska Planina Mts, between Vratsa and Parševitsa hut, FK, AK & VV 2005, rev. SB 43 10'35'' 23 28'55'' 1050 m NC NS 975 ON BG, Petrochan saddle S of Berkovitsa, FK, AK & VV 2005, rev. SB 43 07'05'' 23 07'35'' 1390 m NC NS H. pavichii Heuffel, 2n = 2x 5

6 1004 PAV BG, Central Rodopi Mts, Komuniga village, near the settlement of Jončovo, FK, AK & VV 41 50'20'' 25 11'40'' 770 m NC sexual 2005, rev. SB H. pilosella L., 2n = 4x 69 PI CZ, Krkonoše Mts, Velká Úpa - Janovy Boudy, FK 1995, rev. SB 50 41'20'' 15 48'00'' 860 m 36 a sexual a 120 PI CZ, Krkonoše Mts, Horní Mísečky, FK & AK 1997, rev. SB 50 44'20'' 15 33'50'' 1070 m 36 a sexual a 158 PI CZ, Vršovka near Náchod, FK '30'' 16 07'00'' 330 m 36 c NS 169 PI CZ, Malý Kosíř hill near Slatinice na Hané, FK '30'' 17 05'40'' 270 m 36 c sexual c 191 PI CZ, Krkonoše Mts, Pec pod Sněžkou, FK 1999, rev. SB 50 41'50'' 15 43'50'' 830 m 36 b NS 218 PI CZ, Krkonoše Mts, Horní Malá Úpa Pomezní Boudy, FK, SB & JC '10'' 15 48'00'' 950 m 36 b sexual b 241 PI CZ, Krkonoše Mts, Moravská bouda near Špindlerův Mlýn, FK, SB & JC '10'' 15 36'30'' 1240 m 36 b sexual b 246 PI CZ, Krkonoše Mts, Benecko, FK, SB & JC '00'' 15 33'00'' 800 m 36 b sexual b 268 PI CZ, Šumava Mts, Stožec, FK 1999, rev. SB & FS 48 51'30'' 13 49'20'' 780 m 36 c sexual 300 PI CZ, Praha Vysočany, near the railway station, FK & JC '45'' 14 29'50'' 210 m 36 c sexual 362 PI PL, Góry Izerskie Mts, Jakuszyce, FK 2000, rev. HWS 50 48'45'' 15 23'50'' 830 m 36 d NS 376 PI CZ, Krušné hory Mts, Přebuz, FK '00'' 12 30'00'' 890 m 36 d NS 427 PI CZ, Krkonoše Mts, Víchovská Lhota, FK, AK & JC 2000, rev. SB 50 38'55'' 15 29'15'' 580 m 36 NS 543 PI CZ, Šumava Mts, Hadí vrch near Hartmanice, FP & FK 2001, rev. SB & FS 49 11'05'' 13 20'45'' 960 m 36 sexual 551 PI CZ, Šumava Mts, Slučí Tah near Hartmanice, FP & FK '10'' 13 21'00'' 880 m 36 NS 578 PI CZ, Ještědský hřeben Mts, Kryštofovo Údolí near Chrastava, FK & SB '04'' 14 55'57'' 440 m 36 d NS 585 PI CZ, Bědovice near Týniště nad Orlicí, FK & AK '35'' 16 01'50'' 250 m NC NS 640 PI CZ, České středohoří Mts, Raná hill, FK '35'' 13 46'40'' 320 m NC NS 714 PI CZ, Vrbčany near Kouřim, FK '40'' 14 60'00'' 220 m NC NS 715 PI CZ, Zebín hill near Jičín, FK '15'' 15 22'20'' 390 m NC NS 5/97 PI PL, Beskid Sląski Mts, Ustroń, AK '20'' 18 51'20'' 800 m 36 c NS 734 PI CZ, Šumava Mts, Jelení Vrchy, FP, FK, SB & FS '00'' 13 47'10'' 820 m NC sexual 765 PI CZ, Šumava Mts, Strašín (limestone quarry), FP, FK, SB & FS '00'' 13 37'50'' 590 m NC NS 782 PI CZ, Mnichov near Mariánské Lázně (serpentine rocks), OR '12'' 12 47'07'' 700 m 36 c NS 836 PI D, North Rhine Westfalia, Hagen Eilpe, Krähnocken, above Eilper Hangstieg, UR ' 7 28' 260 m NC NS 841 PI D, North Rhine Westfalia, Hagen Eilpe, Krähnocken, above Eilper Hangstieg, UR ' 7 28' 260 m NC NS H. pilosella L., 2n = 5x 168 PI CZ, Malý Kosíř hill near Slatinice na Hané, FK '30'' 17 05'40'' 270 m 45 c sexual c 273 PI CZ, Valašské Klobouky, FK '10'' 18 02'10'' 510 m 45 c NS 294 PI CZ, Praha Vysočany, near the railway station, FK & JC '45'' 14 29'50'' 210 m 45 c sexual 341 PI CZ, Olomouc - Holice, FK '40'' 17 17'00'' 210 m 45 NS 581/1 PI SK, Nízke Tatry Mts, Borová Sihoť, FK 2001, rev. HWS 49 02'15'' 19 42'20'' 650 m NC NS 587 PI SK, Velká Fatra Mts, Necpalská dolina valley, FK '30'' 19 00'00'' 560 m NC NS 6

7 588 PI SK, Velká Fatra Mts, Tlstá Mt, FK '00'' 18 58'30'' 1370 m NC NS H. pilosella L., 2n = 6x 163 PI CZ, Běstviny near Rychnov nad Kněžnou, FK '30'' 16 08'40'' 310 m 54 c apomictic c 263 PI SK, Velká Fatra Mts, Králova Studňa Mt, FK & AK 1999, rev. HWS 48 52'40'' 19 02'30'' 1270 m 54 c NS 582 PI SK, Chočské pohorie Mts, Prosiek, FK & AK 2001, rev. HWS 49 09'30'' 19 29'55'' 640 m 54 NS 669 PI CZ, Bílý kopec hill near Čejč, FK & VM '25'' 16 59'05'' 190 m NC apomictic 675 PI CZ, Dolní Dunajovice near Mikulov, FK, OR & JD '20'' 16 33'05'' 240 m NC apomictic 686 PI CZ, Sedlec near Mikulov, FK, OR & JD '45'' 16 40'20'' 190 m NC apomictic 693 PI CZ, Brno, Kamenný kopec hill, FK & OR '50'' 16 33'20'' 370 m 54 NS 712 PI CZ, Slavkov near Brno, Rašovice, FK & OR '10'' 16 56'25'' 420 m 54 NS CM 3 PI CZ, Praha, Černý Most, VK 50 06'50'' 14 35'15'' 250 m NC apomictic H. pilosella L., 2n = 7x CM 7 PI CZ, Praha, Černý Most, VK 50 06'50'' 14 35'15'' 250 m 63 (counted by V. Jarolímová) NS Intermediate species H. bifurcum M. Bieb., 2n = 5x 72 (Šobes) CZ, Šobes vineyards near Hnanice, TP m 45 d almost sterile H. blyttianum Fries, 2n = 4x 222 AUxLA CZ, Krkonoše Mts, Dolní Malá Úpa, Moravanka, FK, AK, SB & JCH '50'' 15 49'10'' 1050 m 36 b apomictic b 198 AUxLA CZ, Krkonoše Mts, Horní Malá Úpa - Pomezní Boudy, FK, SB & JCH '20'' 15 48'20'' 1010 m 36 b apomictic b H. brachiatum Bertol. ex DC., 2n = 4x 495 BRA CZ, Praha Vysočany, near the railway station, FK '45'' 14 29'50'' 210 m 36 apomictic 642 BRA CZ, Praha Vysočany, near the railway station, FK & AK '45'' 14 29'50'' 210 m 36 sexual 613 BRA CZ, Roudnice, Drahobuz, FK '30'' 14 20'20'' 220 m NC sexual H. brachiatum Bertol. ex DC., 2n = 5x 667/1 BRA CZ, Kobeřice near Slavkov, FK & OR '35'' 16 53'20'' 330 m 45 apomictic 164 BRA CZ, Slatinice na Hané, FK '40'' 17 05'40'' 250 m 45 c apomictic c H. brachiatum Bertol. ex DC., 2n = 7x 507 BRA CZ, Praha - Vysočany, near the railway station, FK 2001 (4 distinct clones 507,508,513,651) 50 06'45'' 14 29'50'' 210 m 63 apomictic 508 BRA CZ, Praha - Vysočany, near the railway station, FK '45'' 14 29'50'' 210 m 63 apomictic 513 BRA CZ, Praha - Vysočany, near the railway station, FK '45'' 14 29'50'' 210 m 63 apomictic 651 BRA CZ, Praha - Vysočany, near the railway station, FK & AK '45'' 14 29'50'' 210 m 63 apomictic H. brachiatum Bertol. ex DC., 2n = 8x 695 BRA CZ, Brno, Kamenný kopec hill, OR 1999, FK & OR '50'' 16 33'15'' 370 m 72 c apomictic 7

8 1025 BRA CZ, Praha - Vysočany, near the railway station, FK & JC 2000, FK &AK m 72 c apomictic c H. floribundum Wimm. et Grab., 2n = 4x 24 FL CZ, Krkonoše Mts, Velká Úpa - Janovy Boudy, FK 1995, rev. SB 50 41'20'' 15 48'00'' 870 m 36 a apomictic a 250 FL CZ, Šumava Mts, in the former settlement of Stará Hůrka, FP 1999, rev. SB & FS 49 07'30'' 13 19'48'' 1010 m 36 c NS 373 FL CZ, Krušné hory Mts, Přebuz, FK '00'' 12 30'00'' 890 m 36 c NS 379 FL CZ, Krušné hory Mts, Přebuz, FK 2000 (the clone different from 373) 50 24'00'' 12 30'00'' 890 m 36 c NS 384 FL CZ, Krušné hory Mts, Rolava, FK '15'' 12 35'45'' 880 m 36 c NS 466 FL CZ, Českomoravská vrchovina Highlands, Žďár nad Sázavou - railway station, OR '14'' 15 56'20'' 560 m 36 c NS 468 FL PL, Kudowa Zdrój, Lewin Klodzki, RŠ '30'' 16 16'50'' 450 m 36 c NS 879 FL CZ, Šumava Mts, Zhůří near Kašperské Hory, FK 2003, rev. SB & FS 49 05'10'' 13 33'20'' 1120 m NC NS 899 FL CZ, Šumava Mts, Zhůří near Železná Ruda, FK 2003, rev. SB & FS 49 10'50'' 13 20'20'' 950 m NC apomictic 912 FL CZ, Šumava Mts, Gerlova Huť near Železná Ruda, FK 2003, rev. SB & FS 49 09'54'' 13 16'44'' 950 m NC NS H. floribundum Wimm. et Grab., 2n = 5x 580/1 FL SK, Nízke Tatry Mts, Demänovská dolina valley, Jasná pod Chopkom, FK & AK 2001, rev '55'' 19 34'55'' 1200 m NC NS HWS 783 FL PL, distr. Kraków, Gacki settlement near Wielka Wies, OR '13'' 19 50'43'' 400 m NC NS H. glomeratum Froel., 2n = 4x 90 GL CZ, Krkonoše Mts, Horní Malá Úpa - Pomezní Boudy, FK 1997, rev. SB 50 44'20'' 15 48'10'' 960 m 36 a apomictic a H. glomeratum Froel., 2n = 5x 157 GL CZ, Vršovka near Náchod, FK '30'' 16 07'00'' 330 m 45 c NS 576 GL CZ, Ještědský hřeben Mts, Kryštofovo Údolí near Chrastava, FK & SB '04'' 14 55'57'' 440 m NC NS 767 GL CZ, Šumava Mts, Nové Hutě, FP, FK, SB & FS '10'' 13 38'30'' 1010 m NC NS 875 GL CZ, Šumava Mts, Zhůří near Kašperské Hory, FK 2003, rev. SB & FS 49 04'50'' 13 33'20'' 1140 m NC NS 886 GL CZ, Šumava Mts, Filipova Huť, FK 2003, rev. SB & FS 49 01'25'' 13 30'40'' 1060 m NC NS 919 GL SK, Slovenský raj Mts, Stratenská Píla, HWS '30'' 20 21'30'' 800 m NC NS H. iseranum Uechtr., 2n = 4x 117 IS CZ, Krkonoše Mts, Výrovka near Pec pod Sněžkou, FK & AK 1997, rev. SB 50 43'10'' 15 41'00'' 1390 m 36 a apomictic a 4/8 AP CZ, Krkonoše Mts, Velká Úpa - Janovy Boudy, FK & AK 1998, rev. SB 50 41'20'' 15 48'00'' 870 m 36 apomictic 434 IS CZ, Krkonoše Mts, Benecko, FK, AK & JC 2000, rev. SB 50 40'10'' 15 33'00'' 800 m 36 NS 521 IS CZ, Krkonoše Mts, Rýchory - Sklenářovice, FK 2001, rev. SB 50 38'25'' 15 51'10'' 800 m 36 NS ise Gr 2 PL, Góry Izerskie Mts, between Orle and former settlement Gross Iser, SB '42'' 15 22'30'' 810 m 36 NS ise Hot 1 D, Erzgebirge Mts, Schellerhau - Hotel, SB '38'' 13 41'24'' 770 m NC NS ise Neu 12 D, Erzgebirge Mts, Neuwernsdorf, SB '07'' 13 31'17'' 600 m 36 NS H. leptophyton Nägeli et Peter, 2n = 7x 597 LEP CZ, Roudnice, Přestavlky, FK '10'' 14 11'25'' 210 m 63 NS H. macrostolonum G. Schneider, 2n = 6x 8

9 927 MA SK, Slovenský raj Mts, Kopanisko - lúky, HWS 2004, rev. FS 48 54'00'' 20 17'35'' 980 m NC NS H. piloselliflorum Nägeli et Peter, 2n = 4x 227/1 PF CZ, Krkonoše Mts, Dolní Malá Úpa, FK, AK, SB & JC '20'' 15 48'40'' 950 m 36 b apomictic b 238 PF CZ, Krkonoše Mts, Slezské sedlo near Špindlerovka, FK, AK, SB & JC '50'' 15 37'20'' 1200 m 36 b apomictic 431 AP CZ, Krkonoše Mts, Horní Mísečky, FK, AK & JC 2000, rev. SB 50 44'05'' 15 34'10'' 1020 m 36 NS 736 PF CZ, Šumava Mts, Jelení Vrchy, FP, FK, SB & FS '55'' 13 52'45'' 890 m NC sexual 742 PF CZ, Šumava Mts, Hadí vrch near Hartmanice, FP, FK, SB & FS '05'' 13 20'45'' 960 m NC sexual 751 PF CZ, Šumava Mts, Stará Hůrka, FP, FK, SB & FS '30'' 13 19'48'' 1010 m NC sexual 752 PF CZ, Šumava Mts, Slučí Tah near Hartmanice, FP, FK, SB & FS '10'' 13 21'00'' 880 m NC sexual 552 SC CZ, Šumava Mts, Slučí Tah near Hartmanice, FP & FK 2001, rev. SB 49 09'10'' '' 880 m 36 sexual H. piloselliflorum Nägeli et Peter, 2n = 5x 184/3 PF CZ, Kralický Sněžník Mts, Dolní Morava, FK & AK '50'' 16 48'10'' 620 m 45 c NS 213 PF CZ, Krkonoše Mts, Horní Malá Úpa - Pomezní Boudy, FK, AK, SB & JC '30'' 15 48'40'' 1020 m 45 b NS 440 AP CZ, Krkonoše Mts, Horní Malá Úpa - Pomezní Boudy, FK, AK & JC 2000, rev. SB 50 44'20'' 15 48'50'' 1000 m 45 apomictic 370 PF PL, Góry Izerskie Mts, Velká Jizerská louka meadow close to Czech-Polish border, FK 2000, 50 51'00'' 15 21'40'' 830 m 45 d NS rev. HWS 821 PI-FL PL, Jerzmanowice near Kraków, Lysa skala, OR '30'' 19 45'30'' 450 m NC NS H. piloselliflorum Nägeli et Peter, 2n = 6x 224/1 PF CZ, Krkonoše Mts, Dolní Malá Úpa - Rennerovy Boudy, FK, AK, SB & JC '40'' 15 49'20'' 1060 m 54 b apomictic b 230/2 PF CZ, Krkonoše Mts, Dolní Malá Úpa, between the church and Spálený Mlýn, FK, AK, SB & JC 50 42'50'' 15 48'50'' 880 m 54 b apomictic b AP CZ, Krkonoše Mts, Horní Malá Úpa - Pomezní Boudy, FK 1997, rev. SB 50 44'20'' 15 48'50'' 1000 m 54 a apomictic a H. rothianum Wallr., 2n = 4x 10 (Boří les) CZ, Břeclav-Poštorná, near the railway station Boří les, TP, JC & JŠ m 36 c apomictic 9 (Bzenec) CZ, near railway-station Bzenec-Přívoz near Bzenec, TP m 36 c apomictic 36 (Děvín) CZ, Pavlovské kopce hills, Děvín hill, TP m 36 c apomictic 43 (Habří) CZ, České středohoří Mts, Habří, Rač hill, TP m NC apomictic 70 (Havraníky) CZ, Havranické vřesoviště heathland near Havraníky, TP & JC m NC apomictic 66 (Skalky) CZ, Skalky hill near Havraníky, TP m NC apomictic 45 (Trojhora) CZ, České středohoří Mts, Chudoslavice, Trojhora hill, TP m 36 c apomictic H. rubrum Peter, 2n = 6x 549 RU CZ, Šumava Mts, Slučí Tah near Hartmanice, FP & FK 2001, rev. SB & FS 49 09'10'' 13 21'00'' 880 m 54 apomictic 756 RU CZ, Šumava Mts, Zhůří near Železná Ruda, FP, FK, SB & FS '50'' 13 20'20'' 950 m NC apomictic 8/3 RU CZ, Krkonoše Mts, Velká Úpa - Janovy Boudy, FK & AK 1998, rev. SB 50 41'20'' 15 48'00'' 860 m 54 b apomictic b 732 SF CZ, Krkonoše Mts, Velká Úpa - Janovy Boudy, FK 2002, rev. SB 50 41'30'' 15 47'50'' 880 m 54 NS 97 RU CZ, Krkonoše Mts, Horní Malá Úpa - Pomezní Boudy, FK 1997, rev. SB 50 44'20'' 15 48'50'' 1000 m 54 a apomictic a 9

10 C2 RU CZ, Krkonoše Mts, Horní Malá Úpa - Černá Voda, JC & VK '15'' 15 49'20'' 1050 m NC NS H. scandinavicum Dahlst., 2n = 4x 910 GL CZ, Šumava Mts, Gerlova Huť, FK 2003, rev. SB 49 09'54'' 13 16'44'' 950 m NC NS 913 CE CZ, Šumava Mts, Gerlova Huť, FK 2003, rev. SB 49 09'54'' 13 16'44'' 950 m NC apomictic H. schultesii F.W. Schultz, 2n = 3x 735 SC CZ, Šumava Mts, Jelení Vrchy, FP, FK, SB & FS '55'' 13 52'45'' 890 m NC sterile 763/1 SC CZ, Šumava Mts, Horská Kvilda, FP, FK, SB & FS '35'' 13 33'40'' 1130 m NC sterile H. schultesii F.W. Schultz, 2n = 4x 8/11 SC CZ, Krkonoše Mts, Velká Úpa - Janovy Boudy, FK & AK 1998, rev. SB 50 41'20'' 15 48'00'' 860 m 36 apomictic 188 LAxPI CZ, Krkonoše Mts, Pec pod Sněžkou, FK 1999, rev. SB 50 41'50'' 15 43'50'' 830 m 36 b NS 788 SC CZ, Českomoravská vrchovina Highlands, Cikháj near Žďár nad Sázavou, OR '07'' 15 57'54'' 650 m 36 d NS 738 SC CZ, Šumava Mts, Jelení Vrchy, FP, FK, SB & FS '55'' 13 52'45'' 890 m NC sexual 923 SC SK, Slovenský raj Mts, Stratená, meadow area Hanesová, HWS 2004, rev. SB 48 51'15'' 20 18'25'' 910 m NC NS 928 SC SK, Slovenský raj Mts, Stratená, FS '20'' 20 20'40'' 870 m NC NS H. schultesii F.W. Schultz, 2n = 5x 118 SC CZ, Krkonoše Mts, Horní Mísečky, FK & AK 1997, rev. HWS & SB 50 44'10'' 15 34'10'' 1060 m 45 a apomictic a 258/1 SC SK, Velká Fatra Mts, Králova Studňa Mt, FK & AK 1999, rev. HWS 48 52'40'' 19 02'30'' 1270 m 45 c NS 528 SC PL, Massife of Śłęza near Sobótka, PK '40'' 16 43'30'' 290 m NC NS H. stoloniflorum Waldst. et Kit., 2n = 4x 534 SF CZ, Šumava Mts, Zhůří near Kašperské Hory, FP & FK 2001, rev. SB & FS 49 05'10'' 13 33'20'' 1120 m 36 sexual 755 SF CZ, Šumava Mts, Zhůří near Železná Ruda, FP, FK, SB & FS '50'' 13 20'20'' 950 m NC sexual 835/2 SF D, North Rhine Westfalia, Hagen - Eilpe, Krähnocken, above Eilper Hangstieg, UR ' 7 28' 260 m NC sexual Recent hybrids H. aurantiacum H. lactucella, 2n = 3x 818 AuxLA CZ, Vimperk, FP 2002, rev. SB & FS 49 03'20'' 13 46'30'' 700 m 27 NS H. aurantiacum H. rubrum, 2n = 7x 433 AU CZ, Krkonoše Mts, Horní Mísečky, FK, AK & JC '10'' 15 34'00'' 1030 m 63 apomictic H. floribundum H. pilosella, 2n = 6x 743 IS CZ, Šumava Mts, Hadí vrch near Hartmanice, FP, FK, SB & FS '05'' 13 20'45'' 960 m 54 apomictic 823 FL CZ, Českomoravská vrchovina Highlands, Horní Bory near Velké Meziříčí, OR '20'' 16 01'10'' 540 m 54 NS H. lactucella H. onegense, 2n = 2x 6/6 SP4 CZ, Krkonoše Mts, Velká Úpa - Janovy Boudy, FK & AK 1998, rev. SB 50 41'20'' 15 47'40'' 870 m 18 b sexual b 924 LaxON SK, Slovenský raj Mts, Stratená, meadow area Hanesová, HWS '15'' 20 18'25'' 910 m NC NS H. pilosella H. rubrum, 2n = 5x 10

11 220 SF CZ, Krkonoše Mts, Horní Malá Úpa - Pomezní Boudy, FK, SB & JC '10'' 15 48'00'' 950 m 45 b apomictic *Country abbreviations: A Austria; BG Bulgaria; CZ Czech Republic; D Germany; H Hungary; PL Poland; RO Romania; SK Slovak Republic. Collector and/or revisor abbreviations: AK A. Krahulcová; DH D. Hrčka; FK F. Krahulec; FP F. Procházka; FS F. Schuhwerk; HWS participants of the Hieracium Workshop; JC J. Chrtek; JD J. Danihelka; JH J. Hadinec; JŠ J. Škorničková; MC M. Chytrý; OR O. Rotreklová; PK P. Kwiatkowski; PM P. Mráz; RŠ R. Šimek; SB S. Bräutigam; TP T. Peckert; UR U. Raabe; VK V. Košťálová; VM V. Mahelka; VV V. Vladimirov; ZS Z. Szeląg. Chromosome numbers were determined from the same individual as subjected to flow cytometric analyses, except for counts done by R. Šimek and V. Vladimirov that are based on different individual from the same locality. Published counts: a Krahulcová and Krahulec, 1999; b Krahulcová et al., 2001; c Rotreklová et al., 2002; d Rotreklová et al., Unless otherwise indicated, other chromosome numbers were determined by A. Krahulcová. NC = chromosomes not counted. Breeding systems of the plants published (see references a-d above) or under investigation (A. Krahulcová et al., unpubl. res.). 11