Nutritional compositions and yield of sweet fennel cultivars Foeniculum vulgare Mill. ssp. vulgare var. azoricum (Mill.) Thell

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Nutritional compositions and yield of sweet fennel cultivars Foeniculum vulgare Mill. ssp. vulgare var. azoricum (Mill.) Thell M. oudela 1,. Petříková 2 1 Faculty of Agrobiology, Food and tural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic 2 Faculty of Horticulture, Mendel University of Agriculture and Forestry in Brno, Lednice, Czech Republic Abstract: In two-year trials taking place at the Faculty of Horticulture in Lednice (Mendel University of Agriculture and Forestry in Brno), three cultivars of sweet fennel (Foeniculum vulgare Mill. ssp. vulgare var. azoricum [Mill.] Thell) Precoce di Bologna, Rudy F1 and Zefa Fino have been evaluated. The trials were conducted in two terms in each of the trial years (summer and autumn term). In the harvest time, nutritional value was evaluated: content of vitamin C, minerals (,,, ), dietary fibre, dry matter and nitrates. Besides, the weights of s and its yield have been evaluated. The content of the monitored substances ranged in the following intervals: vitamin C 87 347 mg/kg, 4,241 5,851 mg/kg, 77 512 mg/kg, 56 363 mg/kg, 82 389 mg/kg, dietary fibre 5.75 7.59 g/kg, dry matter 61 75.8 g/kg, nitrates 650 to 3,767 mg/kg, weight of 199 383 g. A significant influence of cultivar on content has been observed; furthermore, growing term markedly influenced the content of vitamin C,,,,, dietary fibre, nitrates and on weight of. A significant influence of year on the content of vitamin C,,, dietary fibre, dry matter, nitrates and weight of has been recorded as well. From the three tested cultivars, Zefa Fino showed significantly higher average yield (331 kg/100 m 2 ) in summer terms and Rudy F1 (267 kg/100 m 2 ) in autumn terms. eywords: sweet fennel (Foeniculum vulgare Mill. ssp. vulgare var. azoricum [Mill.] Thell); Precoce di Bologna; Rudy F1; Zefa Fino; vitamin C (ascorbic acid); minerals (,,, ); dietary fibre; dry matter; nitrates; weight of Using less known kinds of vegetables is one of the possibilities to improve unsatisfactorily low vegetable consumption, which is 80 kg/person per year in the Czech Republic, as well as assortment diversity of the consumed vegetables. The latter is also vital because of high content of nutritional components that those less known vegetables contain (opec 1998). In the Czech Republic, sweet fennel is still rather an uncommon kind of vegetable. Its blanched fleshy basal part () is used for consumption. The aim of this work was to screen the values of selected chemical components in different cultivars of sweet fennel and to investigate their yield parameters. Material and Methods Selected cultivars Precoce di Bologna (Sativa Soc. Coop. a r.l.) cultivar recorded in the Czech Republic since 1997. Not suitable for early sowing at the beginning of May; from June sowing, it gives high yield of s, which are suitable for storage (Anonymous 2007). Rudy F1 (Bejo Zaden) uniform, balanced, high yield cultivar, suitable for spring, summer and autumn production. It gives round, shiny, white s, resistant to blooming (Anonymous 2000).

Zefa Fino (Nickerson-Zwaan) very early cultivar; it has soft, medium-size leaves, s are flat spheric, white coloured, relatively resistant against blooming (Anonymous 1994). Terms of sweet fennel growing Summer term sowing: middle of April, outplanting: end of May (growing space 0.5 0.2 m), harvest: beginning of August. Autumn term sowing: beginning of July, outplanting: middle of August (growing space 0.5 0.2 m), harvest: beginning of November. Field trials were conducted in Lednice (altitude 164 m) on a field of the Mendel University of Agriculture and Forestry in Brno in 1998 and 1999. There were three repetitions for each cultivar. Sweet fennel was grown according to the recommended technology (Malý et al. 1998). Plants were harvested in the stage pursuant to the quality standard (ČSN 46 3173). Chemical analyses were done at harvest by taking three plants from each lot. Mixed samples were prepared from the 3 plants. Analytical methods (ascorbic acid) determination by Tillmans titration method; minerals (,,, ) capilary isotachophoresis method (equipment IONOSEP 900.1.); dietary fibre Henneberg-Stohmann method; dry matter drying (temperature of 103 ± 2 C); nitrates ion selective electrode (ISE). Investigated chemical components and single plant weight were statistically evaluated using ANOVA method (at the level of α = 0.5 and α = 0.01). PC programme Unistat (Version 4.53) was used for statistical evaluation. Results and discussion Differences between years, terms and cultivars in selected chemical compounds and s weights Recorded values of vitamin C content ranged from 87 to 347 mg/kg in the fennel (Tables 1 and 2). The cultivar Precoce di Bologna reached the highest values in all terms (summer and autumn of both years); the differences of vitamin C content among cultivars were however not significant (Table 3). Contrary to this, significant differences in vitamin C content among lettuce cultivars were found out by Petříková and Pokluda (2003), among Chinese cabbage by Malý (2003). Growing period and year nevertheless significantly influenced vitamin C content (higher values were in autumn terms) (Tables 4 and 5). Values of vitamin C content recorded in this work are similar to those presented by Dobromilska (1996); she reported vitamin C content values in incrassate bottom part of stems at the level of 140 200 mg/kg. Minerals (,,, ) Mineral content in fennel ranged in the following intervals: (4,241 5,851 mg/kg), (77 512 mg/kg), (56 363 mg/kg), (82 389 mg/kg) (Tables 1 and 2). Potassium The highest values of (5,851 mg/kg) were found in the Precoce di Bologna cultivar in the autumn growing term of 1998, and in the Rudy F1 cultivar (5,568 mg/kg) in the autumn term of 1999. Values of content were significantly higher: in the autumn term of 1998 than in the summer term of that year (Table 4), Table 1. Chemical components of sweet fennel (in fresh matter) Dietary fibre Dry matter Cultivar (mg/kg) (g/kg) (mg/kg) Summer 1998 Precoce di Bologna 102 4,610 79 61 92 6.62 67 1,537 Rudy F1 87 4,322 77 58 85 6.51 65 1,177 Zefa Fino 89 4,393 84 56 82 5.96 63 1,717 Autumn 1998 Precoce di Bologna 347 5,851 239 224 199 6.65 67 3,767 Rudy F1 124 5,187 356 280 174 5.75 61 3,250 Zefa Fino 278 4,899 293 209 141 6.55 65 3,100

Table 2. Chemical components of sweet fennel (in fresh matter) Cultivar Summer 1999 Dietary fibre Dry matter (mg/kg) (g/kg) (mg/kg) Precoce di Bologna 120 4,241 218 140 152 7.14 68 713 Rudy F1 112 4,948 196 156 166 7.30 70 783 Zefa Fino 91 4,807 195 160 212 6.82 68 650 Autumn 1999 Precoce di Bologna 151 4,272 512 322 339 6.37 68 2,167 Rudy F1 129 5,568 281 167 389 6.52 68 3,173 Zefa Fino 148 5,266 495 363 359 7.59 76 3,023 in the Rudy F1 cultivar than in the Precoce di Bologna cultivar in 1999. No significant influence of year or cultivar on content was found in both (1998, 1999) summer and autumn growing terms (Tables 3 and 5). Significantly higher content of in autumn cultivation term of head lettuce was reported by Petříková et al. (2004). Sodium The Rudy F1 cultivar reached the highest values of content in the autumn term of 1998 (356 mg/kg) as well as the Precoce di Bologna cultivar in the autumn term of 1999 (512 mg/kg). There were statistically significantly higher values of content in autumn terms when compared to summer terms of each year (1998 and 1999). No statistically significant influence of year and cultivar was observed on content of summer or autumn terms (Tables 1 to 5). lcium The highest content was recorded in the Rudy F1 cultivar in the autumn term of 1998 (280 mg/kg) and in the Zefa Fino cultivar in the autumn term of 1999 (363 mg/kg). Significantly higher values of content were found in autumn than in summer growing terms in both years. Similarly, Petříková et al. (2004) mentioned significantly higher content of in head lettuce in the autumn growing term in comparison to summer growing term. The influence of year was significant in summer growing terms (Tables 1, 2, 4 and 5). The influence of cultivar on content was not confirmed in either of the terms (1998 and 1999). Magnesium The highest content was found in the Precoce di Bologna in the autumn term of 1998 (199 mg/kg) and in the Rudy F1 cultivar in the autumn term of 1999 (389 mg/kg). Values of content were significantly higher in autumn than in summer growing terms in both years (Tables 1, 2, 4 and 5). Significant influence of year on content in both growing terms was found, whereas no significant influence of cultivar on content was observed (Table 3). Table 3. Significance of differences in chemical composition and weight in sweet fennel cultivars Precoce di Bologna Rudy F1 Zefa Fino Rudy F1 + + significant difference at P = 0.05

Table 4. Significance of differences between terms Summer Table 5. Significance of differences between years 1998 Autumn ++ ++ ++ ++ ++ + ++ ++ 1999 + ++ ++ + + ++ ++ + significant difference at P = 0.05, ++ significant difference at P = 0.01 + significant difference at P = 0.05, ++ significant difference at P = 0.01 Shumaila et al. (2005) presented the following values of minerals in dry matter of sweet fennel : 133.33 mg/g, 80 mg/g, 0.62 mg/g, 19.81 mg/g. In the case of, and, their values exceed values from our trials several times (after recomputation to dry matter). On the contrary, content mentioned by Shumaila et al. (2005) showed several times lower values. opec (1998) mentioned the following values of mineral contents in fennel: 4,940 mg/kg, 860 mg/kg, 1,090 mg/kg, and 80 mg/kg. The values mentioned by opec (1998), except for and, are similar to the results reported in this work. content was significantly influenced by growing term and cultivar; content was significantly influenced by growing term, and and content by growing term and year. The highest content of dietary fibre was found in the Precoce di Bologna in the autumn term of 1998 (6.65 g/kg) and in the Zefa Fino cultivar in the autumn term of 1999 (7.59 g/kg). Values of dietary fibre content were significantly higher in the summer than in the autumn term of 1999. In summer terms, statistically significant influence of year on dietary fibre content was found (Tables 1, 2, 4 and 5). It could have been caused by a lower level of rainfalls in summer terms, resulting in higher formation of dietary fibre in summer growing terms, particularly in 1999. Results of dietary fibre content in this work ranged from 5.75 g/kg to 7.59 g/kg; they are several times lower than the values (approximately 33 g/kg) mentioned by opec (1998). The results of dietary fibre content were significantly influenced by growing term and year. The highest content of dry matter was shown by the Precoce di Bologna cultivar in 1998 (67 g/kg) and the Zefa Fino cultivar in the autumn term of 1999 (76 g/kg). A significant influence of growing term and cultivar on dry matter content in sweet fennel was not confirmed, while a significant influence of year was found (Tables 1 to 5). The highest content of nitrates was found in the Precoce di Bologna in the autumn growing term of 1998 (3,767 mg/kg) and in the Rudy F1 cultivar in the autumn growing term of 1999 (3,173 mg/kg). Table 6. Pseudobulb weight (g) Cultivar Summer Autumn 1998 1999 average 1998 1999 average Precoce di Bologna 353 300 327 218 240 229 Rudy F1 337 199 268 283 251 267 Zefa Fino 383 278 331 219 222 221 Spacing 0.5 0.2 m for sweet fennel (10 pieces/m 2 )

Significantly higher values of nitrate contents were found in autumn when compared with summer growing terms 1998 and 1999. There was a significant influence of year on nitrate content in autumn and summer growing terms (Tables 1, 2, 4 and 5). The observed values ranging from 650 mg/kg to 3,767 mg/kg were higher in both growing terms of 1998 and in the autumn growing term of 1999 than the maximum limit stipulated by the tional decree 53/2002 Coll. (1,000 mg/kg for leafy vegetable). Lower values were reached only in summer 1999. An intensive negative influence of low light intensity on nitrate accumulation was noted. This corresponds with rezel and oota (2003) and other authors. Lipska-Szpunar (1992) mentioned the nitrate level in sweet fennel from 4,261 to 4,300 mg/kg. These relatively high values are due to the conditions in high plastic tunnels where the higher content of nitrates is caused by lower light intensity when compared with field conditions., yield of s (kg/100 m 2 ) The highest value of single weight was found in the Zefa Fino cultivar in the summer growing term of 1998 (383 g) and in the Precoce di Bologna cultivar in the summer growing term of 1999 (300 g). Pseudobulbs weights ranged from 199 g to 383 g. Values of s weights were significantly higher in 1998 summer growing term in comparison with autumn. A significant influence of year on weight was observed in summer growing terms (Tables 4 6). The Precoce di Bologna cultivar showed the highest average yield from all growing terms (278 kg for 100 m 2 ). Differences among average yield were minimal. The ascertained values of sweet fennel yield correspond with values 200 300 kg/100 m 2 listed by Petříková et al. (2006). CONCLUSION Levels of vitamin C, chlorophyll,,,,, dietary fibre, dry matter and nitrates were measured in sweet fennel (Foeniculum vulgare Mill. ssp. vulgare var. azoricum [Mill.] Thell). The following values of monitored parameters were recorded: vitamin C 87 347 mg/kg, 4,241 to 5,851 mg/kg, 77 512 mg/kg, 56 363 mg/kg, 82 389 mg/kg, dietary fibre 5.75 7.59 g/kg, dry matter 61.0 75.8 g/kg, nitrates 650 3,767 mg/kg, weight 199 383 g. Cultivar significantly influenced only the content of. Factors that influenced the monitored parameters (nutritional compounds and weight) of sweet fennel more significantly were growing term (spring and summer) and year. R e f e r e n c e s ANONYMOUS, 1994. Assortment list of vegetable seeds 1994 1995. Barendrecht, Nickerson Zwaan. ANONYMOUS, 2000. Gartenkatalog 2000. Marbach, Hild samen GmbH: 60. ANONYMOUS, 2007. Available at: http://www.semo.cz/english/main.php?section=1&id= a21 ČSN 46 3173, 1998. Fenykl sladký. Praha, Český normalizační institut. DOBROMILSA R., 1996. The influence of the cultivar and seed spacing on the yield and quality of fennel. In: Nowe rośliny i technologie w ogrodnictwie. Poznań, Akademia Rolnicza w Poznaniu: 103 106. (In Polish) opec., 1998. Tabulky nutričních hodnot ovoce a zeleniny. Praha, ÚZPI: 72. REZEL J., OOTA E., 2003. Yield evaluation of some Chinese cabbage cultivars in spring and autumn cultivation. Folia Horticulturae, 15: 11 18. LIPSA-SZPUNAR M., 1992. Possibilities of growing Florence fennel (Foeniculum vulgare Mill. var. azoricum Thell) in high plastics tunnels. Biuletyn Warzywniczy, 11: 51 59. (In Polish) MALÝ I., 2003. valita produkce vybraných odrůd pekingského a čínského zelí. In: Zborník vedeckých prác Udržateľné poľnohospodárstvo a rozvoj vidieka. Nitra, SPU: 324 326. Malý I. et al., 1998. Polní zelinářství. Praha, Agrospoj: 196. PETŘÍOVÁ., POLUDA R., 2003. Vliv odrůdy a doby pěstování na nutriční hodnoty hlávkového salátu. In: Sborník referátů z česko-slovenské konference valita rostlinné produkce: současnost a perspektivy směrem k EU. Praha, VÚRV: 123 126. PETŘÍOVÁ., MALÝ I., POLUDA R., PACÍ V., 2004. Integrované pěstování listové zeleniny. Praha, ÚZPI, Zemědělské informace č. 4: 42. PETŘÍOVÁ., JÁNSÝ J., MALÝ I., PEZA Z., POLÁČOVÁ J., ROD J., 2006. Zelenina (pěstování ekonomika prodej). Praha, Profi Press: 161 162. SHUMAILA., NAZISH J., SOFIA N., HALIL R., 2005. Mineral profile of Foeniculum vulgare (Fennel) and Eugenia caryophyllus (clove). Indus Journal of Biological Sciences, 2: 246 249. No. 53/2002 Coll. Decree of the Ministry of Health of the Czech Republic stipulating chemical requirements for health safety of individual kinds of foods and food sources, requirements for using additives and food supplements. April 10, 2004. Received for publication April 19, 2007 Accepted after corrections July 27, 2007

Nutriční hodnota a výnos u fenyklu sladkého Foeniculum vulgare Mill. ssp. vulgare var. azoricum (Mill.) Thell ABSTRAT: Ve dvouletých pokusech, které probíhaly na Zahradnické fakultě v Lednici (Mendelova zemědělská a lesnická univerzita v Brně), byly hodnoceny tři odrůdy fenyklu sladkého hlíznatého (Foeniculum vulgare Mill. ssp. vulgare var. azoricum [Mill.] Thell) Precoce di Bologna, Rudy F1 a Zefa Fino. Pokusy byly uskutečněny ve dvou termínech pro každý pokusný rok (letní a podzimní termín). V době sklizně byla hodnocena nutriční hodnota: obsah vitaminu C, minerálních látek (,,, ), vlákniny, sušiny a dusičnanů. Byla také hodnocena hmotnost nepravých cibulí a výnos. Obsah sledovaných látek se pohyboval v následujících intervalech: vitamin C 87 až 347 mg/kg, 4 241 5 851 mg/kg, 77 512 mg/kg, 56 363 mg/kg, 82 389 mg/kg, vláknina 5,75 až 7,59 g/kg, sušina 61 75,8 g/kg, dusičnany 650 3 767 mg/kg, hmotnost nepravých cibulí 199 383 g. Byl zjištěn průkazný vliv odrůdy na obsah. Termín pěstování významně ovlivnil obsah vitaminu C,,,,, vlákniny, dusičnanů a hmotnost nepravé hlízy. Rovněž byl zaznamenán průkazný vliv roku na obsah vitaminu C,,, vlákniny, sušiny, dusičnanů a hmotnost nepravé hlízy. Ze tří testovaných odrůd vykazovala nejvyšší průměrný výnos v letních termínech odrůda Zefa Fino (331 kg/100 m 2 ), zatímco v podzimních termínech pěstování vykazovala nejvyšší výnos odrůda Rudy F1 (267 kg/100 m 2 ). líčová slova: fenykl sladký hlíznatý (Foeniculum vulgare Mill. ssp. vulgare var. azoricum [Mill.] Thell); Precoce di Bologna; Rudy F1; Zefa Fino; vitamin C (kyselina askorbová); minerální látky (,,, ); vláknina; sušina; dusičnany; hmotnost nepravé cibule Corresponding author: Ing. Martin oudela, Ph.D., Česká zemědělská univerzita v Praze, Fakulta agrobiologie, potravinových a přírodních zdrojů, 165 21 Praha 6-Suchdol, Česká republika tel.: + 420 224 382 553, fax: + 420 224 382 557, e-mail: koudela@af.czu.cz