Program of the XVIII. Workshop of Biophysical Chemists and Electrochemists

Program of the XVIII. Workshop of Biophysical Chemists and Electrochemists 12 th and 13 th September, 2018 under the auspices of the rector of Masaryk University, Assoc. Prof. PhDr. Mikuláš Bek, Ph.D. and the dean of Faculty of Science MU, Assoc. Prof. Mgr. Tomáš Kašparovský, Ph.D. Motto: I am among those who think that science has great beauty. A scientist in his laboratory is not only a technician: he is also a child placed before natural phenomena which impress him like a fairy tale. Marie Curie - Sklodowska

SPONSORS The main sponsor 2

Dear Colleagues, On behalf of the Organizing Committee I am honored to welcome you in Brno, from Wednesday 12 September (afternoon) to Thursday 13 September (whole day), for the 18 th Workshop of (Bio)physical Chemists and Electrochemists. The conference is held at Masaryk University (MU) in its new Campus (Kamenice 5, Bohunice, building A12). It is worth mentioning that the University Campus Bohunice (UCB) is beginning to enjoy the status of a prestigious undertaking within the development of the city of Brno as well as attracting the ever increasing interest of the commercial sector. An entirely new district with complete urban infrastructure has emerged in the immediate vicinity; the Campus Square a shopping center opened in 2008. Although the UCB naturally serves all of Masaryk University, it is primarily designed to accommodate three faculties: Faculty of Science, Faculty of Medicine and Faculty of Sports Studies. The university research and education parts of these faculties were primarily financed from the European Union Structural Funds. The three significant projects (CEITEC, CESEB, CETOCOEN) within the campus area provided background for a range of scientific disciplines focused e.g. on biomedical technologies. The CEITEC (i.e. Central European Institute of Technology) project was granted by the European Commission 6 June 2011. It emerged from a common project of six most significant Brno universities and research institutions, with the support of the South Moravian Region and the City of Brno. The major scope of the project is to establish an internationally acclaimed center of research and education. Modern laboratories were erected in the university campus complex in 2014. State-of-the-art technologies installed in them enable synergetic studies of both biotic and abiotic components of the environment on all currently available levels of complexity, ranging from single atoms, through molecules, molecular ensembles or cells, up to whole organisms including humans. Owing to the CESEB (Centre of Experimental, Systematic and Ecological Biology) project, students, teachers and scientists take advantage of four brand new pavilions allowing parallel activities in both instruction and scientific research of two departments of the Faculty of Science: Department of Experimental Biology and Department of Botany and Zoology. The project is beneficial to all fields of the Biology programs, especially by increasing the number and capacity of premises designed for practical tuition. The Centre for the Study of Toxic Substances (CETOCOEN) project covers the development of chemical and toxic tools monitoring the quality of environment. The Centre is a result of an ambitious project aimed at establishing a unique platform interconnecting scientific research and commercial sphere in the areas of environmental and sustainability issues. This year s conference is dedicated to application of biophysical chemistry and electrochemistry in proteomics, genomics, bioanalysis and biotechnology. According to the attached program you can see the schedule of plenary lectures, lectures, presentations of young scientists, presentations of posters and other events. The both conference days are opened with the two (Wednesday) or three (Thursday) plenary lectures, followed by the invited lectures. All participants are invited to the Poster Session followed by the gala dinner on Wednesday early in the evening. The Thursday s afternoon (13 th September) is devoted to competitive lectures in the Young Scientists Session. The conference will be concluded with the announcement of three winners of the Young Scientists Session and one winner of the Poster Session for the most interesting research results and their presentation. We are confident that our Workshop will be memorable for its scientific quality, also thanks to your contributions. We do hope that you will welcome the opportunity to present and discuss your scientific and educational results. Welcome to Brno and enjoy the conference! Libuše Trnková 3

12:00 12:50 REGISTRATION 13:00 13:10 OPENING CEREMONY Libuše Trnková, Jan Hrbáč Wednesday 12 th September, 2018 PLENARY LECTURES Chairman Prof. RNDr. Libuše Trnková, CSc. 13:10 13:45 Zbyněk Prokop MICROFLUIDICS IN PROTEIN CHEMISTRY 13:45 14:20 Miroslav Macka ELECTROCHEMICAL DETECTION IN MINIATURISED CAPILLARY SEPARATION PLATFORMS: GAP CELL FOR AMPEROMETRIC DETECTION FOR PORTABLE LIQUID CHROMATOGRAPHY 14:20 14:40 COFFEE BREAK LECTURES Chairman Assoc. Prof. RNDr. Jan Hrbáč, Ph.D. 14:40 15:00 Veronika Ostatná CHRONOPOTENTIOMETRIC ANALYSIS OF METHYLATED AND ACETYLATED BOVINE SERUM ALBUMIN 15:00 15:20 Aleš Daňhel SILVER AMALGAM PARTICLES IN SPECTRO-ELECTROCHEMICAL APPLICATIONS 15:20 15:40 Rastislav Ošťádal THERMO FISCHER SCIENTIFIC. AQUILOS FIRST FIB/SEM FOR CRYO- LAMELLA PREPARATION IN LIFE SCIENCE 15:40 16:15 Traditional photo and coffee break 16:15 17:15 PRESENTATION OF COMPANIES 17:15-18:15 POSTER SESSION Chairman Prof. RNDr. Libuše Trnková, CSc. Presentations of posters THE AUTHORS OF POSTERS REGISTRED IN COMPETITION WILL PREPARE A SHORT (max. 3 MINUTES) PRESENTATION AT THEIR POSTERS. THE AUTHORS OF THE OTHER POSTERS WILL PRESENT THEIR POSTERS INDIVIDUALLY. 18:15 WELCOME PARTY 4

Thursday 13 th September, 2018 PLENARY LECTURES Chairman Assoc. Prof. RNDr. Jan Hrbáč, Ph.D. 9:00 9:35 Martin Pumera ELECTROCHEMISTRY OF 2D NANOMATERIALS 9:35 10:10 Cina Foroutannejad SINGLE-MOLECULAR MEMRISTORS 10:10 10:45 František Foret DISPLACEMENT ELECTROPHORESIS FOR LARGE VOLUME DNA CONCENTRATION 10:45 11:05 COFFEE BREAK LECTURES Chairman Assoc. Prof. RNDr. Petr Skládal, CSc. 11:05 11:25 Jan Hrbáč PRODUCTION OF NANOMATERIALS BY SPARK DISCHARGE AND ITS USE FOR THE PREPARATION OF ELECTROCHEMICAL SENSORS 11:25 11:45 Zdeněk Farka NANOPARTICLE-BASED IMMUNOCHEMICAL ASSAYS: NANO-UPDATE OF CLASSIC ANAYLTICAL TOOLS 11:45 12:00 Karel Lacina ENHANCEMENT OF THE OUTPUT VOLTAGE OF A GALVANIC CELL USING GALVANIC SHORT CIRCUIT 12:00 12:20 Peter Barath METROHM GET TO KNOW METROHM 12:20 13:20 LUNCH YOUNG SCIENTISTS SESSION Chairman RNDr. Alan Liška, Ph. D. 13:30 13:45 Zuzana Mikušová DEVELOPMENT OF AMPEROMETRIC IMMUNOSENSOR FOR DIAGNOSIS OF ALBUMINURIA BASED ON SCREEN - PRINTED ELECTRODES 13:45 14:00 Martin Konhefr SYNTHESIS, STRUCTURAL CHARACTERISATION AND ELECTROCHEMISTRY OF DIFERROCENYLBORONIC ACID 5

14:00 14:15 Matěj Pastucha PATHOGENIC BACTERIA DETECTION USING ELECTROCHEMICAL IMMUNOSENSORS 14:15 14:30 Jakub Sopoušek BIOSENSING BASED ON ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY: INFLUENCE OF THE OFTEN-IGNORED MOLECULAR CHARGE 14:30 14:45 Jakub Věžník DIFFERENCES OF REDOX BEHAVIOUR OF FERROCENE DERIVATIVES IN BUFFERED AND UNBUFFERED AQUEOUS SOLUTIONS 14:45 15:00 Filip Květoň APPLICATION OF GLYCAN BIOSENSORS IN DIAGNOSTICS 15:00 15:15 COFFEE BREAK YOUNG SCIENTISTS SESSION Chairman Mgr. Martin Konhefr 15:15 15:30 Alan Liška STEREOCHEMICAL INVESTIGATION OF CALIXARENES WITH VARIOUS BRIDGING UNITS 15:30 15:45 Petra Itterheimová USE OF DODECABENZYLBAMBUS[6]URIL IN PREPARATION OF PERCHLORATE ION-SELECTIVE ELECTRODES 15:45 16:00 Erika Gedeonová TOWARDS THE UNDERSTANDING OF CELL DEATH DURING ALZHEIMER'S DISEASE: INVESTIGATION OF ERAB AND CYPD INTERACTION 16:00 16:15 Jan Slavík ELECTROCHEMICAL DETERMINATIV OF METHALLOTHIONEIN IN PRESENCE OF SODIUM SELENITE BY BRDICKA REACTION 16:15 16:30 Michael Gargulák THERANOSTIC APPROACH TO COPING WITH SERIOUS BACTERIAL INFECTIONS: USE OF SILVER NANOPARTICLES 16:30 16:50 COFFEE BREAK 16:50 ANNOUNCEMENT OF THE COMPETITION WINNERS Libuše Trnková, Peter Barath, Iveta Třísková, Jan Hrbáč ANNOUNCEMENT OF THE COMPETITION RESULTS FOR THE BEST POSTERS ANNOUNCEMENT OF THE COMPETITION RESULTS OF THE YOUNG SCIENTISTS SESSION The sponsor of the book prizes: Czech Chemical Society, subdivision Brno. 6

List of posters 1. INTERACTION OF HUMAN GUT MICROBIOTA WITH SELECTED FLAVONOIDS Petr HODEK, Pavlína HUCKOVÁ, Jakub MRÁZEK, Jiří HUDEČEK, Marie STIBOROVÁ 2. ENTRANCE OF APOFERRITIN WITH ENCAPSULATED ELLIPTICINE INTO CELLS AND EFFECT OF THIS NANOPARTICLE ON THESE CELLS Radek INDRA, Tereza ČERNÁ, Jan HRABĚTA, Simona DOSTÁLOVÁ, Zbyněk HEGER, Vojtěch ADAM, Tomáš ECKSCHLAGER, Marie STIBOROVÁ 3. CONSTRUCTION OF APOFERRITIN - A NANOTRANSPORTER WITH AN ENCAPSULATED INHIBITOR OF TYROSINE KINASE VANDETANIBE Radek INDRA, Kateřina JÁKLOVÁ, Simona DOSTÁLOVÁ, Zbyněk HEGER, Vojtěch ADAM, Marie STIBOROVÁ 4. ELECTROCHEMICAL ANALYSIS OF CdTe QUANTUM DOTS FOR UV RADIATION DETECTION Bozena HOSNEDLOVA, Michaela DOCEKALOVA, Martina STANKOVA, Dagmar UHLIROVA, Branislav RUTTKAY-NEDECKY, Marta KEPINSKA, Halina MILNEROWICZ, Nguyen Xuan HAI, Duong Ngoc BACH, Hoai Viet NGUYEN, Jiri SOCHOR, Josef RUZICKA and Rene KIZEK 5. ANTICANCER DRUGS VANDETANIB, LENVATINIB AND CABOZANTINIB MODULATE OXIDATION OF AN ELLIPTICINE ANTITUMOR AGENT CATALYZED BY CYTOCHROMES P450 AND PEROXIDASES Matúš KOLÁRIK, Radek INDRA, Vojtěch ADAM, Zbyněk HEGER, Marie STIBOROVÁ 6. DESIALYLATION OF AIRWAY EPITHELIUM INCREASES BINDING OF PSEUDOMONAS AERUGINOSA LECTINS PAIL AND PAIIL Božena KUBÍČKOVÁ, Jana RYCHNOVÁ, Kateřina DOSTÁLOVÁ, Kateřina VYHNALOVÁ, Jana MRÁZKOVÁ, Václav MANDYS, Michaela WIMMEROVÁ, Marie STIBOROVÁ, Petr HODEK 7. DEPENDENCE OF ANTIBACTERIAL PROPERTIES OF SILVER NANOPARTICLES ON THEIR SURFACE MODIFICATION Branislav RUTTKAY-NEDECKÝ, Božena HOSNEDLOVÁ, Michaela DOČEKALOVÁ, Dagmar UHLÍŘOVÁ, Martina STAŇKOVÁ, Marta KEPINSKA, Josef RŮŽIČKA, Carlos FERNANDEZ, Halina MILNEROWICZ, René KIZEK 8. THE BIOPHYSICAL STUDY OF NEWLY DESIGNED FULLERENE- DOXORUBICIN NANOTRANSPORTER CONJUGATES FOR TARGETED BREAST CANCER THERAPY Sylvie SKALIČKOVÁ, Marta KEPINSKA, Michael GARGULÁK, Michaela DOČEKALOVÁ, Božena HOSNEDLOVÁ, Branislav RUTTKAY-NEDECKÝ, Halina MILNEROWICZ, Rene KIZEK 9. FORMATION OF DNA ADDUCTS BY PLANT ALKALOID ARISTOLOCHIC ACID AS A CASUAL REASON EXPLAINING INITIATION OF UPPER UROTHELIAL CANCER Marie STIBOROVA, Volker M. ARLT, Heinz H. SCHMEISER 7

10. OXIDATION OF TYROSINE KINASE INHIBITOR VANDETANIB BY HUMAN CYTOCHROMES P450 AND FLAVIN MONOOXYGENASES AND ITS EFFECT ON GENERATION OF DNA ADDUCTS BY AN ANTICANCER DRUG ELLIPTICINE Radek INDRA, Petr POMPACH, Paulína TAKÁCSOVÁ, Katarina VAVROVÁ, Zbyněk HEGER, Vojtěch ADAM, Tomáš ECKSCHLAGER, Kateřina KOPEČKOVÁ, Václav MARTÍNEK, Marie STIBOROVÁ 11. ELECTROCHEMICAL ANALYSIS OF DNA INTERACTION WITH GOLD NANOPARTICLES Andre FERREIRA DA SILVA, Zuzana TOTHOVA, Dagmar UHLIROVA, Christina KOLLIOPOULOU, Amastasia SEIMENI, Michael GARGULAK, Michaela DOČEKALOVÁ, Branislav RUTTKAY-NEDECKÝ, Rene KIZEK 12. REDOX PROCESSES OF DNA FRAGMENT dg3 Iveta TŘÍSKOVÁ, Libuše TRNKOVÁ, Alan LIŠKA 13. A RAPID METHOD FOR THE SARCOSINE DETECTION USING PSEUDO- PEROXIDASE ACTIVITY OF SUPERPARAMAGNETIC IRON OXIDE NANOPARTICLES (SPION) Dagmar UHLÍŘOVÁ, Martina STAŇKOVÁ, Michaela DOČEKALOVÁ, Božena HOSNEDLOVÁ, Marta KEPINSKA, Branislav RUTTKAY-NEDECKÝ, Josef RŮŽIČKA, Carlos FERNANDEZ, Halina MILNEROWICZ, René KIZEK 14. OXIDATION OF TYROSIN KINASE INHIBITOR LENVATINIB BY HUMAN CYTOCHROMES P450 IN VITRO Katarina VAVROVÁ, Radek INDRA, Petr POMPACH, Zbyněk HEGER, Vojtěch ADAM, Tomáš ECKSCHLAGER, Kateřina KOPEČKOVÁ, Marie STIBOROVÁ 15. PREPARATION OF AN APOFERRITIN NANOTRANSPORTER BEARING ANTICANCER DRUG ELLIPTICINE Radek INDRA, Marek WILHELM, Zbyněk HEGER, Simona DOSTÁLOVÁ, Vojtěch ADAM, Marie STIBOROVÁ 16. STUDY OF RETENTION BEHAVIOR OF ANIONS BY ION CHROMATOGRAPHY Lukáš ZIMA, Marta FARKOVÁ, Zdeněk FARKA, Přemysl LUBAL 8

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