Aplikace elektricky zvlákněných materiálů Electrospun materials Utilization - Applications. Eva Kuželová Košťáková KNT, FT, TUL

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1 Aplikace elektricky zvlákněných materiálů Electrospun materials Utilization - Applications Eva Kuželová Košťáková KNT, FT, TUL

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3 2005 Ramakrishna S., An introduction to Electrospinning and Nanofibers

4 Statistics on the literature published on the advanced applications of electrospun fibers in the period (search made through Scifinder: Keywords Electrospinning + area of application).

5 FILTRATION Air

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7 WOUND DRESSING Fabrics of nanofibers can be used to cover wounds, where they provide oxygen to penetrate, drainage of exudate while preventing bacteria from entering the wound. At the same time they can be connected some supporting materials, such as antimicrobial and hemostatic drug or medicament for accelerating the healing process;

8 Sound absorbtion Basic fibrous material with the nanofibrous layers of different area densities (colored curves) vs. Basic mat erial without t he nanofibers (black curve) 1 0,8 0,6 0,4 0, f [Hz] basic material nano-1 g.m-2 nano-0,5 g.m-2 nano-0,1 g.m-2

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10 Agriculture The dosage of fertilizers

11 Membranes for clothing industry

12 Membranes for clothing industry Permeability Breathability (vapor permeability) hydrostatic pressure (the water column) Wind resistance

13 Membranes for clothing industry A nanofiber membrane made by NANOPROTEX can boast of extremely high steam permeability, which is given by the unique nanofiber structure, and a nanofiber diameter which is only 150 nm (those are the finest fibres ever used in the textile industry). The research team found additional inspiration for nanofiber membrane development in human skin, which enables the so-called insensible perspiration. Thanks to this structure and nanofiber diameter the NANOPROTEX nanomembranes have more pores per given area compared to microporous membranes. This results in the steam permeability of the 2-layer laminate (face fabric + nanofiber membrane) Ret below 1.5 Pa.m2.W-1. The steam permeability of the nanofiber membrane itself is Ret 0.0 Pa.m2.W-1.

14 Membranes for clothing industry

15 Membranes for clothing industry

16 Membranes for clothing industry NANODRY EASY Nanocity - obuv s touto membránou je nepromokavá a prodyšná. Doporučujeme pro uživatele, kteří ocení lehkou obuv do města i do přírody. Zajistí příjemné klima za mírných a teplých podmínek a to i při intenzivnějších nebo extrémních aktivitách. Nohy budou stále suché, teplé a v pohodlí při použití ve vnitřních prostorách i venku. NANODRY ARCTIC Nanoforest Winter - vysoce tepelně izolační čtyřvrstvý laminát předurčuje jeho použití v obuvi v chladných zimních podmínkách. Je trvale nepromokavá a prodyšná, navržena pro pohyb v mokru, v dešti, na sněhu a ledu.

17 Litvínov Nanovia AntiAllergy textilie bariéra proti alergenům. Inovativní textilie zabraňující průniku prachových roztočů a jimi produkovaných alergenů na základě principu jejich mechanického záchytu. Přednosti antialergických bariérových textilií s nanovlákennou vrstvou oproti tkaným bariérovým textiliím Materiály Nanovia AntiAllergy jsou jediné 100% účinné textilní bariéry proti prostupu alergenů z lůžka a lůžkovin. Poskytují výborný fyziologický komfort při svém použítí díky výborné prodyšnosti a paropropustnosti. Jedná se o materiály vyrobené nejmodernějšími textilními technologiemi, které využívá unikátní vlastnosti nanovláken.

18 Cosmetics - Nafigate

19 O NAFIGATE Cosmetics Jsme česká společnost, která propojuje vědu s kosmetikou. Díky výzkumu nanotechnologií jsme vyvinuli jedinečné přípravky, na nanovlákennou podoční masku Nano Eye Lift jsme dokonce získali patent. Při výrobě našich produktů využíváme různě velké molekuly kyseliny hyaluronové. Ladislav Mareš zakladatel

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21 541-ceske-nanotechnologie-jsouvyhlasene-firmy-presto-tezce-shanejiinvestory

22 Drug delivery systems

23 Tissue Engineering

24 Tissue Engineering The temporary structure built around a building for the "convenience" of work of employees What is the scaffold? The construction for the cultivation of cells and tissues.

25 Tissue Engineering

26 PES nanofibers and stem cells Tissue Engineering

27 Nonwoven material Textile scaffold In vitro testing Electrospun material - PCL

28 PCL electrospun nanofibers (3D structure + hydroxyapatite) with fibroblasts after 10 days cultivation in-vitro

29 Aligned and non-uniform nanofibrous structure with cells a b c d Zhong, S., Teo, W., E., Zhu, X., Beuerman, R., W., Ramakrishna, S., Yung, L., Y., L.: An aligned nanofibrous collagen scaffold by electrospinning and its effects on in vitro fibroblast culture, Journal of Biomedical Materials Research Part A, pg , 2006,

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31 PLA polylactid acid E. Košťáková, KNT, FT, TUL Nejpoužívanější materiály pro výrobu elektrostaticky zvlákněných materiálů užívaných v tkáňovém inženýrství: PGA polyglycolic acid PLGA copolymer of lactid and glycolic acid PCL poly- -caprolactone Chitosan Gelatine Collagen Etc.

32 Nanofibers Production, Properties and Functional Applications 290 Fig. 3. The cross-sectional images of a double-layered small diameter PU vascular graft before (a) and after (b) cell culturing (Blue, nuclei; green, F-action; red,scaffold) (Soletti et al., 2010).

33 A scaffold production In vitro testing Implantation in vivo testing

34 Normal and new cartilage Textile scaffold In vivo testing 80x

35 Textile scaffold In vivo testing Mag. 40x New cartilage Old cartilage Chondrocytes has to penetrated to the scaffold, created structures typical for cartilage (collage type II), has to treat osteochondral defects..

36 Materiály pro tkáňové inženýrství vyvíjené na KNT, FT, TUL Představení současného výzkumu Jana Horáková, Věra Jenčová, Petr Mikeš, Jakub Erben, Kateřina Strnadová, Jiří Chvojka, Jiří Havlíček, Ondřej Novák, Eva Kuželová Košťáková, Filip Sanetrník, David Lukáš,

37 Department of Nonwovens and Nanofibrous Materials Small diameter vascular grafts Maloprůměrové cévní náhrady

38 Department of Nonwovens and Nanofibrous Materials SMALL DIAMETER VACULAR GRAFT Double-layered vascular graft nanofibers in the inner layer oriented microfibers in the outer layer Cross-sections of doublelayered vascular graft made from polycaprolactone

39 Department of Nonwovens and Nanofibrous Materials SMALL DIAMETER VACULAR GRAFT Vascular graft made from polylactide-polycaprolactone copolymer Cross-section Morphology of fibers Excellent mechanical properties: ultimate tensile strength up to 10 MPa elongation at break up to 500 %

40 Department of Nonwovens and Nanofibrous Materials SMALL DIAMETER VACULAR GRAFT In vivo tests I Rat animal model replacement of abdominal aorta, 6 months implantation PCL nanofibrous vascular graft PCL nanofibrous vascular graft modified by NO releasing compound

41 Department of Nonwovens and Nanofibrous Materials SMALL DIAMETER VACULAR GRAFT In vivo tests II Rabbit animal model - carotid artery bypass PLC vascular graft

42 Department of Nonwovens and Nanofibrous Materials Nanofibrous wound dressings Kryty ran

43 Department of Nonwovens and Nanofibrous Materials NANOFIBROUS WOUND DRESSINGS Nanofibrous material high surface area porous structure structure stimulate cell attachement and proliferation biodegradable biocompatible (NHDF)

44 absorbance (570nm) Department of Nonwovens and Nanofibrous Materials NANOFIBROUS WOUND DRESSINGS In vitro tests - cytotoxicyty, biocompatibility Cells Tests normal human dermal fibroblasts (NHDF) mouse fibroblasts (3T3) cell viability - MTT cell adhession, proliferation microscopy (fluorescence, SEM) 1D Results (scaffold): 3D Fluorescence microscopy: NHDF cells on the material, PI staining of the cell nuclei. non cytotoxic biocompatible (with used fibroblasts) capable for the cell adhession promote cell proliferation 1 0,9 0,8 0,7 0,6 0,5 0,4 0,3 0,2 0, čas (dny) PCL - 3T3 PCL-PRP-3T

45 Clinical studies NANOFIBROUS WOUND DRESSINGS chronic and accute wounds first patiens promising results clinical study in process (in 2015) before After 3 months 1D 3D

46 Department of Nonwovens and Nanofibrous Materials 3D micro-nano fibrous scaffolds prepared by meltblwon and electrospinning combination for the bone tissue engineering KOMBINACE MIKRO A NANOVLÁKEN PRO TKÁŇOVÉ INŽENÝRSTVÍ KOSTÍ

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48 Department of Nonwovens and Nanofibrous Materials BONE REGENERATION MELTBLOWN+ELSPIN A novel scaffold produced by combining meltblown and electrospinning technology. It is possible to fill in in-situ particle in-between fibers was developed. The scaffold has sufficient surface properties and porous structure. This scaffold is beneficial for cell growth, adhesion and proliferation and may be served as bone substitutes in tissue engineering application. Further studies are focused on its implantation into animal models for the investigation of its behavior in vivo. 1D 3D

49 Department of Nonwovens and Nanofibrous Materials BONE REGENERATION MELTBLOWN+ELSPIN Patented technology. Administration Utility Model: Device for manufacturing a composite textile material comprising polymer nanofibres 1D 3D

50 Department of Nonwovens and Nanofibrous Materials BONE REGENERATION MELTBLOWN+ELSPIN Analysis of the impact of technological parameters combination of electrospinning and meltblown technology. 1D 3D

51 Department of Nonwovens and Nanofibrous Materials BONE REGENERATION MELTBLOWN+ELSPIN Scaffold fibrous material for the preparation of bone substitutes combining micro- and nanofibers. 1D 3D

52 absorbance at 570 nm Department of Nonwovens and Nanofibrous Materials BONE REGENERATION MELTBLOWN+ELSPIN Were completed in-vitro tests with positive results. 12 1D 3D 10 8 M 6 4 MS ME MES time [days]

53 Department of Nonwovens and Nanofibrous Materials BONE REGENERATION MELTBLOWN+ELSPIN Were launched in-vivo tests and we have positive results 1D 3D

54 Department of Nonwovens and Nanofibrous Materials BONE REGENERATION MELTBLOWN+ELSPIN 1D 3D Materials Letters, March , Vol.143, pg , doi: /j.matlet , (IF 2.269)

55 Department of Nonwovens and Nanofibrous Materials KOMBINACE 3D TISKU A VZOROVANÝCH NANOVLÁKEN - CHRUPAVKY 1D 3D

56 Department of Nonwovens and Nanofibrous Materials Patented combination of technologies 3D PRINTING PATTERNED ELECTROSPUN LAYER CARTILAGE REGENERATION 3D PRINT+ELSPIN FINAL PRODUCT 1D 3D

57 Department of Nonwovens and Nanofibrous Materials In-vitro testing Migration of mesenchymal stem cells throught smooth (classical) and patterned nanofibrous electrospun layer Result: Cell can migrate through the patterned electrospun layer CARTILAGE REGENERATION 3D PRINT+ELSPIN a) b) c) d) Confocal microscopic observation of cells penetrating through the nanofibrous layers of the 2D (c) and patterned (d) layer on day 7; cells were stained using DiOC6 (green) and propidium iodide (red). Colour-coded projection of cells adhered to the 2D (a) and 3D (b) scaffolds on day 7. Scale bar 200 micrometers. 09:

58 Day 28 Day 28 Day 28 Day 7 Day 7 Day 7 Day 1 Day 1 Day 1 Department of Nonwovens and Nanofibrous Materials CARTILAGE REGENERATION 3D PRINT+ELSPIN In-vitro testing - very good cells viability. - Structure allows cells penetration into the scaffold. - structure stimulates chondrocytes to synthesize procollagen II. - The 3D printed grid consists of a sparse structure which facilitates the penetration of the cell and supplying the scaffold mechanical resistance (strength) and provides sufficient space for the formation of new tissue Cell visualization Color coded Production of collagen II Blue colour = cells onto electrospun layers

59 Department of Nonwovens and Nanofibrous Materials CARTILAGE REGENERATION 3D PRINT+ELSPIN In-vivo testing with positive results

60 Department of Nonwovens and Nanofibrous Materials CARTILAGE REGENERATION 3D PRINT+ELSPIN Changes of structure morphology 3D printing Patterned elspin layers Final composition The technology combination has potential also in the bone regeneration.

61 Department of Nonwovens and Nanofibrous Materials DRUG DELIVERY SYSTEMS SYSTÉMY PRO ŘÍZENÉ DODÁVÁNÍ LÉČIV ELECTROSPRAYING Coaxial electrospraying/controled degradation

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64 The electrospinning is capable to prepare novel materials with enhanced electrochemical properties of the primary functional components (electrolyte, anode and cathode) of lithium ion batteries. Principle of LiFePO 4 charge/discharge process in lithium ion battery # Schematic representation of charging and discharging processes of lithium ion battery with PE based on ESM.