Jiří Novotný SEZNAM PUBLIKACÍ 1) Vědecké monografie 2) Kapitoly v monografiích 3) Původní vědecké práce v mezinárodních časopisech s recenzním řízením 1. Nemcova I., Novotny J. and Horska V. (1986) Spectrophotometric study of phenothiazine derivatives and their cation radicals in micelar media. Microchem. J. 34:180-189. IF (2011) 3.048 2. Nemcova I., Masojidkova M. and Novotny J. (1988) An NMR study of the interaction of phenothiazine derivatives with cationic and anionic tensides. Microchem. J. 38:80-85. IF (2011) 3.048 3. Novotny J. (1990) Properties and use of mumps virus antigen for detection of specific IgG and IgM antibodies in enzyme-linked immunosorbent assay. Acta Virol. 34:574-577. IF (2011) 0.682 4. Novotny J., Arenberger P. and Matouskova E. (1991) Cultivation of psoriatic keratinocytes and an attempt to isolate and detect a specific psoriatic antigen. Folia Biol. 37:52-54. IF (2011) 1.151 5. Fedova D., Novotny J. and Kubinova I. (1992) Serological diagnosis of parainfluenza virus infections. Verification of the sensitivity and specifity of the hemagglutination-inhibition (HI), complement-fixation (CF), immunofluorescence (IFA) tests and enzyme immunoassay (ELISA). Acta Virol. 36:304-312. IF (2011) 0.682 6. Novotny J., Svobodova J., Ransnäs L.A. and Kubistova K. (1992) A method for the preparation of purified antigens of coxsackievirus B3 from a large volume of cell culture supernatant. Acta Virol. 36:483-487. IF (2011) 0.682 7. Kvapil P., Novotny J. and Ransnäs L.A. (1994) Activated G s but not G i prevents the thermal inactivation of adenylyl cyclase in plasma membranes derived from S49 lymphoma cells. FEBS Lett. 343:208-212. IF (2011) 3.538 8. Novotny J., Gustafson B., Kvapil P. and Ransnäs L.A. (1994) Adenovirus infection of myocardial cells induces an enhanced sensitivity to -adrenergic agonists by increasing the concentration of the stimulatory G-protein. Biochem. Mol. Biol. Int. 34:993-1001. IF (2001) 0.925 9. Novotny J., Kvapil P., Svobodova J., Cello J. and Ransnäs L.A. (1994) Coxsackie B3 virus penetration into rat cardiocytes alters the adenylyl cyclase signalling system. Biomed. Res. 15:114-122. IF (2011) 1.231 10. Novotny, J., Kvapil, P., Cello, J. and Ransnäs, L.A. (1994) Coxsackievirus B3 entry into the host cell interferes with G-protein-mediated transmembrane signalling. Bioscience Rep. 14:205-214. IF (2011) 2.379 11. Kvapil P., Novotny J., Svoboda P. and Ransnäs L.A. (1994) The short and long forms of the subunit of the stimulatory guanine nucleotide-binding protein are unequally redistributed during (-)-isoproterenol mediated desensitization of intact S49 lymphoma cells. Eur. J. Biochem. 226:193-199. IF (2011) 3.790 12. Novotny J., Kvapil P., Bokoch G.M. and Ransnäs L.A. (1995) Isoproterenol-induced subcellular redistribution of G-protein subunits in S49 lymphoma cells demonstrated by a novel competitive ELISA. Arch. Physiol. Biochem. 103:202-210. IF (2000) 0.841 1
13. Kvapil P., Novotny J. and Ransnäs L.A. (1995) Prolonged exposure of hamsters to cold changes the levels of G proteins in brown adipose tissue plasma membranes. Life Sci. 57:311-318. IF (2011) 2.527 14. Novotny J., Kvapil P., Jelinek F. and Ransnäs L.A. (1995) Alterations in G-protein-regulated transmembrane signalling induced in murine myocardium by coxsackievirus B3 infection.cardiovasc. Res. 30:602-610. IF (2011) 6.064 15. Novotny J., Gustafson G. and Ransnäs L.A. (1996) Inhibition of -adrenergic receptor-mediated signals by a synthetic peptide derived from the subunit of the stimulatory G-protein. Biochem. Biophys. Res. Commun. 219:619-624. IF (2011) 2.484 16. Drmota T., Novotny J., Kim G.-D., Eidne K.A., Milligan G. and Svoboda P. (1998) Agonistinduced internalization of the G protein G 11 and thyrotropin-releasing hormone receptors proceed on different time scales. J. Biol. Chem. 273:21699-21707. IF (2011) 4.773 17. Schonning K., Bolmstedt A., Novotny J., Lund O.S., Olofsson S. and Hansen, J.-E.S. (1998) Induction of antibodies against epitopes inaccessible on the HIV type 1 envelope oligomer by immunization with recombinant monomeric glycoprotein 120. AIDS Res. Hum. Retrov. 14:1451-1456. IF (2011) 2.246 18. Bourova L, Novotny J. and Svoboda P. (1999) The decrease in the short variant of G s protein is associated with an increase in [ 3 H]CGP 12177 binding, [ 3 H]ouabain binding and Na,K-ATPase activity in brown adipose tissue plasma membranes of cold- acclimated hamsters. J. Mol. Endocrinol. 22:55-64. IF (2011) 3.485 19. Novotny J., Bourova L., Malkova O., Svoboda P. and Kolar F. (1999) G proteins, - adrenoceptors and -adrenergic responsiveness in immature and adult rat ventricular myocardium: influence of neonatal hypo- and hyperthyroidism. J. Mol. Cell. Cardiol. 31:761-772. IF (2011) 5.166 20. Novotny J., Krusek J., Drmota T. and Svoboda P. (1999) Overexpression of the G protein G 11 prevents desensitization of Ca 2+ response to thyrotropin-releasing hormone. Life Sci. 65:889-900. IF (2011) 2.527 21. Drmota T., Novotny J., Gould G.W., Svoboda P. and Milligan G. (1999) Visualization of distinct patterns of subcellular redistribution of the thyrotropin-releasing hormone receptor-1 and G q /G 11 induced by agonist stimulation. Biochem. J. 340:529-538. IF (2011) 4.897 22. Bourova L., Novotny J. and Svoboda P. (1999) Resolution and identification of G q /G 11 and G i /G o proteins in brown adipose tissue: effect of cold acclimation. J. Mol. Endocrinol. 23:223-229. IF (2011) 3.485 23. Pesanova Z., Novotny J., Cerny J., Milligan G. and Svoboda P. (1999) Thyrotropin-releasing hormone-induced depletion of G q /G 11 proteins from detergent-insensitive membrane domains. FEBS Lett. 464:35-40. IF (2011) 3.538 24. Kalmusova J., Novotny J., Hulinska D., Musilek M. and Kriz P. (2000) Interactions of invasive and noninvasive strains of Neisseria meningitidis with monkey epithelial cells, mouse monocytes and human macrophages. Microbiologica 23:185-200. IF (2011) 0.529 2
25. Bourova L., Pesanova Z., Novotny J., Bengtsson T. and Svoboda P. (2000) Differentiation of cultured brown adipocytes is associated with a selective increase in the short variant of G s protein. Evidence for higher functional activity of G s S. Mol. Cell. Endocrinol. 167:23-31. IF (2011) 2.786 26. Novotny J., Bourova, L., Kolar, F and Svoboda P. (2001) Membrane-bound and cytosolic forms of heterotrimeric G proteins in young and adult rat myocardium: influence of neonatal hypo- and hyperthyroidism. J. Cell. Biochem. 82:215-224. IF (2011) 2.868 27. Ihnatovych I., Hejnova L., Kostrnova A., Mares P., Svoboda P. and Novotny J. (2001) Maturation of rat brain is accompanied by differential expression of the long and short splice variants of G s protein. Identification of cytosolic forms of G s. J. Neurochem. 79:88-97. IF (2011) 4.604 28. Ihnatovych I., Novotny J., Haugvicova R., Bourova L., Mares P. and Svoboda P. (2002) Opposing changes of trimeric G proteins levels during ontogenetic development of rat brain. Dev. Brain Res. 133:57-67. IF (2007) 1.783 29. Ihnatovych I., Novotny J., Haugvicova R., Bourova L., Mares P. and Svoboda P. (2002) Ontogenetic development of the G protein-mediated adenylyl cyclase signalling in rat brain. Dev. Brain Res. 133:69-75. IF (2007) 1.783 30. Flachs P., Novotny J., Baumruk F., Bardova K., Bourova L., Miksik I., Svoboda P., Sponarova J. and Kopecky J. (2002) Impaired norepinephrine-induced lipolysis in white fat of a P2-Ucp1 transgenic mice is associated with changes in G-protein levels. Biochem. J. 364:369-376. IF (2011) 4.897 31. Hejnova L., Ihnatovych I., Novotny J., Kubova H., Mares P. and Svoboda P. (2002) Modulation of adenylyl cyclase activity by baclofen in the developing rat brain: difference between cortex, thalamus and hippocampus. Neurosci. Lett. 330:9-12. IF (2011) 2.105 32. Novotny J., Hrbasova M., Kolar F. and Svoboda P. (2003) Cardiomegaly induced by pressure overload in newborn rats is accompanied by altered expression of the long isoform of G s protein and deranged signaling of adenylyl cyclase. Mol. Cell. Biochem. 245:157-166. IF (2011) 2.057 33. Bourova L., Kostrnova A., Hejnova L., Moravcova Z., Moon H.E., Novotny J., Milligan G. and Svoboda P. (2003) -Opioid receptors exhibit high efficiency when activating trimeric G proteins in membrane domains. J. Neurochem. 85:34-49. IF (2011) 4.604 34. Hrbasova M., Novotny J., Hejnova L., Kolar F., Neckar J. and Svoboda P. (2003) Altered myocardial G s protein and adenylyl cyclase signaling in rats exposed to chronic hypoxia and normoxic recovery. J. Appl. Physiol. 94:2423-2432. IF (2011) 3.753 35. Matousek P., Novotny J. and Svoboda P. (2004) Resolution of G s and G q /G 11 proteins in membrane domains by two-dimensional electrophoresis. The effect of long-term agonist stimulation. Physiol. Res. 53:295-303. 36. Moravcova Z., Rudajev V., Stöhr J., Novotny J., Cerny J., Patrenti M., Milligan G. and Svoboda P. (2004) Long-term agonist stimulation of IP prostanoid receptor depletes the cognate G s protein in membrane domains but does not change the receptor level. Biochim. Biophys. Acta 1691:51-65. IF (2011) 2.379 37. Stöhr J., Bourova L., Hejnova L., Ihnatovych I., Novotny J. and Svoboda P. (2004) Increased baclofen-stimulated G protein coupling and deactivation in rat brain cortex during development. Dev. Brain Res. 151:67-73. IF (2071) 1.783 3
38. Matousek P., Novotny J., Rudajev, V. and Svoboda P. (2005) Prolonged agonist stimulation does not alter the protein composition of membrane domains in spite of dramatic changes induced in a specific signaling cascade. Cell Biochem. Biophys. 42:21-40. IF (2011) 3.743 39. Stöhr J., Novotny J., Bourova L. and Svoboda P. (2005) Modulation of adenylyl cyclase activity in young and adult rat brain cortex. Identification of suramin as a direct inhibitor of adenylyl cyclase. J. Cell. Mol. Med. 9:940-952. IF (2011) 4.125 40. Stöhr J., Novotny J. and Svoboda P. (2005) Characterization of [ 3 H]-forskolin binding sites in young and adult rat brain cortex. Identification of suramin as a competitive inhibitor of [ 3 H]- forskolin binding. Can. J. Physiol. Pharmacol. 83:573-581. IF (2011) 1.953 41. Rudajev V., Novotny J., Hejnova L., Milligan G. and Svoboda P. (2005) Dominant portion of thyrotropin-releasing hormone receptor is excluded from lipid domains. Detergent-resistant and detergent-sensitive pools of TRH receptor and G q /G 11 protein. J. Biochem. 138:111-125. IF (2011) 2.371 42. Matousek P., Durchankova D., Svandova I., Novotny J. and Svoboda P. (2005) Agonist-induced tyrosin phosphorylation of G q /G 11 requires the intact structure of membrane domains. Biochem. Biophys. Res. Commun. 328:1526-532. IF (2011) 2.484 43. Novotny J., Durchankova D., Ward R.J., Carrillo, J.J., Svoboda P. and Milligan G. (2006) Functional interactions between the 1b -adrenoceptor and G 11 are compromised by depalmitoylation of the G protein but not of the receptor. Cell. Signal. 18:1244-1251. IF (2011) 4.058 44. Ostasov P., Bourova L., Hejnova L, Novotny J. and Svoboda P. (2007) Disruption of the plasma membrane integrity by cholesterol depletion impairs effectiveness of TRH receptor-mediated signal transduction via G q /G 11 proteins. J. Recept. Signal Transduction 27:335-352. IF (2011) 1.588 45. Ostasov P., Krusek J., Durchankova D., Svoboda P. and Novotny J. (2008) Ca 2+ responses to thyrotropin-releasing hormone and angiotensin II. The role of plasma membrane integrity and effect of G 11 protein overexpression in homologous and heterologous desensitization. Cell. Biochem. Funct. 26:264-274. IF (2011) 1.771 46. Durchankova D., Novotny J. and Svoboda P. (2008) The time-course of agonist-induced solubilization of trimeric G q /G 11 proteins resolved by two-dimensional electrophoresis. Physiol. Res.57:195-203. 47. Bourova L., Stöhr J., Lisy V., Rudajev V., Novotny J. and Svoboda P. (2009) G-protein activity in percoll-purified plasma membranes, bulk plasma membranes, and low-density plasma membranes isolated from rat cerebral cortex. Med. Sci. Monit.15:111-122. IF (2009) 1.699 48. Drastichova Z., Bourova L., Hejnova L., Jedelsky P., Svoboda P. and Novotny J. (2010) Protein alterations induced by long-term agonist treatment of HEK293 cells expressing thyrotropinreleasing hormone receptor and G 11 α protein. J. Cell. Biochem. 109:255-264. IF (2011) 2.868 49. Bourova L., Vosahlikova M.,Kagan D., Dlouha K., Novotny J. and Svoboda P. (2010) Longterm adaptation to high doses of morphine causes desensitization of mu-or- and delta-orstimulated G-protein response in forebrain cortex but does not decrease the amount of G-protein alpha subunits. Med. Sci. Monit.16:BR260-270. IF (2009) 1.699 4
50. Drastichova Z., Skrabalova J., Neckar J., Kolar F., Novotny J. (2011) Prolonged morphine administration alters protein expression in the rat myocardium. J. Biomed. Sci. 18:89. doi: 10.1186/1423-0127-18-89 IF (2011) 1.980 51. Drastichova Z, Novotny J. (2012) Identification of a preassembled TRH receptor-g q/11 protein complex in HEK293 cells. Cell Struct. Funct. 37:1-12. IF (2011) 2.292 52. Skrabalova J., Neckar J., Hejnova L., Bartonova I., Kolar F. and Novotny J. (2012) Antiarrhytmic effect of prolonged morphine exposure is accompanied by alterded myocardial adenylyl cyclase signaling in rats. Pharmacol. Rep. 64:351-359. IF (2011) 2.445 53. Drastichova Z, Novotny J. (2012) Identification and subcellular localization of molecular complexes of G q/11 α protein in HEK293 cells. Acta Biochim. Biophys. Sin. 44:641-649. IF (2011) 1.376 4) Učebnice a učební texty 5) Různé závažné práce 1. Novotný J., Plevka A. (1990) Detekce a kvantifikace antiparotitických IgG protilátek v lidských sérech využití soupravy Sevatest ELISA anti-pav IgG Micro I. Zprávy SEVAC 3:92-96. 2. Novotný J., Brynychová D. (1991) Detekce a kvantifikace antiparotitických IgM protilátek v lidských sérech využití soupravy Sevatest ELISA anti-pav IgM Micro I. Zprávy SEVAC 1:15-20. 6) Přednášky typu invited speakers 7) Přehledy a souborné referáty 1. Novotny J. and Svoboda, P. (1998) The long (G s -L ) and short (G s -S ) variants of the stimulatory guanine nucleotide-binding protein. Do they behave in an identical way? J. Mol. Endocrinol. 20:163-173. IF (2011) 3.485 2. Svoboda P. and Novotny J. (2002) Hormone-induced subcellular redistribution of trimeric G proteins. Cell. Mol. Life Sci. 59:501-512. IF (2011) 6.570 3. Svoboda P., Teisinger J., Novotny J., Bourova L., Drmota T., Hejnova L., Moravcova Z., Lisy V., Rudajev V., Stöhr J., Vokurkova A., Svandova I. and Durchankova D. (2004) Biochemistry of transmembrane signaling mediated by trimeric G proteins. Physiol. Res. 53:S141-S152. 4. Drastichová Z., Bourova L., Lisy V., Hejnova L., Rudajev V., Stöhr J., Durchanková D., Ostasov P., Teisinger J., Soukup T., Novotny J., Svoboda P. (2008) Subcellular redistribution of trimeric G- proteins potential mechanism of desensitization of hormone response: internalization, solubilization, down-regulation. Physiol Res. 57:S1-S10. 8) Patenty národní a zahraniční, objevy 9) Disertační, rigorosní, habilitační (u profesorského řízení) práce Kandidátská disertační práce: Studies on the G-protein-mediated adenylyl cyclase signalling during hormonal stimulation and viral infection, Akedemie věd České republiky, Praha 1996. Doktorská disertační práce: Fyziologické regulace transmembránových signálních systémů řízených trimenrími G-proteiny, Akademie věd České republiky, Praha 2005. 5
10) Abstrakta ze sjezdů a sympozií a odborných setkání (obě formy odlišit) 11) Udělené granty s uvedením názvu a grantové agentury Řešitel: Fyziologický význam dlouhé a krátké varianty G s proteinu, GAČR - 305/01/1660 (2000-2002) Molekulární mechanismy nepříznivého a protektivního působení morfínu a methadonu na srdce potkana, GA AV ČR - IAA501110901 (2009-2013) Úloha adrenergní signalizace a oxidativního stresu v molekulárních mechanismech kardioprotektivního působení chronické hypoxie, GA AV ČR - IAAX01110901 (2009-2013) Člen řešitelského týmu: Centrum molekulární fyziologie a imunologie, MŠMT - VS97099 (1997-2000), řešitel doc. Petr Svoboda The role of membrane domains in heterotrimeric G-protein-mediated hormone action and desensitisation, The Welcome Trust - Collaborative Research Initiative (1999-2004), řešitel doc. Petr Svoboda Centrum molekulární a buněčné imunologie, MŠMT - LN00A026 (2000-2004), řešitel prof. Václav Hořejší Regulace a signalizace v živých systémech, MŠMT - MSM113100003 (2000-2004), řešitel prof. Stanislav Zadražil Úloha trimerních GTP vazebných proteinů v mechanismu hormonální akce, GAČR - 309/01/0255 (2001-2003), řešitel doc. Petr Svoboda Subcellulární distribuce μ- a δ-opiátových receptorů a funkční aktivita trimerních G proteinů po dlouhodobé stimulaci morfiem, GA ČR - 309/06/0121 (2006-2008), řešitel doc. Petr Svoboda Analýza lokalizace delta-opioidního receptoru v membránových doménách úloha palmitoylace, GA AV ČR - IAA500110606 (2006-2008), řešitel doc. Petr Svoboda Buněčná a molekulární fyziologie kardiomyocytů, MŠMT - MEB0810151 (2010-2011), řešitel dr. Žurmanová Signalizace a molekulární mechanismy buněčné odpovědi, MŠMT - MSM0021620858 (2007-2013), řešitel prof. Jan Tachezy Úloha proteinů STAT v cirkadiánním systému potkana GAČR - P303/12/1137 (2012-2014), řešitel dr. Zdeňka Bendová Interakce mezi dlouhodobými formami kardioprotekce vyvolané adaptací na chronickou hypoxii a pravidelnou fyzickou zátěží. GAČR - P303/12/1162 (2012-2015), řešitel prof. František Kolář 12) Ostatní publikace 1. Novotný J. (2006) Stručný přehled transmembránové signalizace řízené trimérními G-proteiny. Česk. Fyziol. 55:128-137. V Praze 10.9.2012 RNDr. Jiří Novotný, DSc. 6